Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Artículo Open Access |
| Published: |
Wiley
2024
|
| Subjects: | |
| Online Access: | https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.70021 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1867017765755289601 |
|---|---|
| author | Peiling Zhang Jinyang Li Xiuhong Gou Lin Zhu Yang Yang Yilin Li Yingqi Zhang Liwen Ding Assiya Ansabayeva Yuling Meng Weixing Shan |
| author_facet | Peiling Zhang Jinyang Li Xiuhong Gou Lin Zhu Yang Yang Yilin Li Yingqi Zhang Liwen Ding Assiya Ansabayeva Yuling Meng Weixing Shan Peiling Zhang Jinyang Li Xiuhong Gou Lin Zhu Yang Yang Yilin Li Yingqi Zhang Liwen Ding Assiya Ansabayeva Yuling Meng Weixing Shan |
| collection | Wiley Open Access |
| contents | The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility Peiling Zhang Jinyang Li Xiuhong Gou Lin Zhu Yang Yang Yilin Li Yingqi Zhang Liwen Ding Assiya Ansabayeva Yuling Meng Weixing Shan Molecular Plant Pathology Abstract Phytophthora infestans is a notorious oomycete pathogen that causes potato late blight. It secretes numerous effector proteins to manipulate host immunity. Understanding mechanisms underlying their host cell manipulation is crucial for developing disease resistance strategies. Here, we report that the conserved RXLR effector Pi05910 of P. infestans is a genotype‐specific avirulence elicitor on potato variety Longshu 12 and contributes virulence by suppressing and destabilizing host glycolate oxidase StGOX4. By performing co‐immunoprecipitation, yeast‐two‐hybrid assays, luciferase complementation imaging, bimolecular fluorescence complementation and isothermal titration calorimetry assays, we identified and confirmed potato StGOX4 as a target of Pi05910. Further analysis revealed that StGOX4 and its homologue NbGOX4 are positive immune regulators against P. infestans , as indicated by infection assays on potato and Nicotiana benthamiana overexpressing StGOX4 and TRV‐ NbGOX4 plants. StGOX4 ‐mediated disease resistance involves enhanced reactive oxygen species accumulation and activated the salicylic acid signalling pathway. Pi05910 binding inhibited enzymatic activity and destabilized StGOX4. Furthermore, mutagenesis analyses indicated that the 25th residue (tyrosine, Y25) of StGOX4 mediates Pi05910 binding and is required for its immune function. Our results revealed that the core RXLR effector of P. infestans Pi05910 suppresses plant immunity by targeting StGOX4, which results in decreased enzymatic activity and protein accumulation, leading to enhanced plant susceptibility. 10.1111/mpp.70021 http://creativecommons.org/licenses/by-nc/4.0/ |
| doi_str_mv | 10.1111/mpp.70021 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_mpp_70021 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by-nc/4.0/ |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility Peiling Zhang Jinyang Li Xiuhong Gou Lin Zhu Yang Yang Yilin Li Yingqi Zhang Liwen Ding Assiya Ansabayeva Yuling Meng Weixing Shan Molecular Plant Pathology The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility Peiling Zhang Jinyang Li Xiuhong Gou Lin Zhu Yang Yang Yilin Li Yingqi Zhang Liwen Ding Assiya Ansabayeva Yuling Meng Weixing Shan Molecular Plant Pathology Abstract Phytophthora infestans is a notorious oomycete pathogen that causes potato late blight. It secretes numerous effector proteins to manipulate host immunity. Understanding mechanisms underlying their host cell manipulation is crucial for developing disease resistance strategies. Here, we report that the conserved RXLR effector Pi05910 of P. infestans is a genotype‐specific avirulence elicitor on potato variety Longshu 12 and contributes virulence by suppressing and destabilizing host glycolate oxidase StGOX4. By performing co‐immunoprecipitation, yeast‐two‐hybrid assays, luciferase complementation imaging, bimolecular fluorescence complementation and isothermal titration calorimetry assays, we identified and confirmed potato StGOX4 as a target of Pi05910. Further analysis revealed that StGOX4 and its homologue NbGOX4 are positive immune regulators against P. infestans , as indicated by infection assays on potato and Nicotiana benthamiana overexpressing StGOX4 and TRV‐ NbGOX4 plants. StGOX4 ‐mediated disease resistance involves enhanced reactive oxygen species accumulation and activated the salicylic acid signalling pathway. Pi05910 binding inhibited enzymatic activity and destabilized StGOX4. Furthermore, mutagenesis analyses indicated that the 25th residue (tyrosine, Y25) of StGOX4 mediates Pi05910 binding and is required for its immune function. Our results revealed that the core RXLR effector of P. infestans Pi05910 suppresses plant immunity by targeting StGOX4, which results in decreased enzymatic activity and protein accumulation, leading to enhanced plant susceptibility. 10.1111/mpp.70021 http://creativecommons.org/licenses/by-nc/4.0/ |
| title | The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility |
| topic | Molecular Plant Pathology |
| url | https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.70021 |