LbSakA‐mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development
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2024
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| author | Liang Shi Zi Wang Ju Hong Chen Hao Qiu Wei Dong Liu Xiao Yan Zhang Francis M. Martin Ming Wen Zhao |
| author_facet | Liang Shi Zi Wang Ju Hong Chen Hao Qiu Wei Dong Liu Xiao Yan Zhang Francis M. Martin Ming Wen Zhao Liang Shi Zi Wang Ju Hong Chen Hao Qiu Wei Dong Liu Xiao Yan Zhang Francis M. Martin Ming Wen Zhao |
| collection | Wiley Open Access |
| contents | LbSakA‐mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development Liang Shi Zi Wang Ju Hong Chen Hao Qiu Wei Dong Liu Xiao Yan Zhang Francis M. Martin Ming Wen Zhao New Phytologist Summary Ectomycorrhizal symbiosis, which involves mutually beneficial interactions between soil fungi and tree roots, is essential for promoting tree growth. To establish this symbiotic relationship, fungal symbionts must initiate and sustain mutualistic interactions with host plants while avoiding host defense responses. This study investigated the role of reactive oxygen species (ROS) generated by fungal NADPH oxidase (Nox) in the development of Laccaria bicolor/Populus tremula × alba symbiosis. Our findings revealed that L. bicolor LbNox expression was significantly higher in ectomycorrhizal roots than in free‐living mycelia. RNAi was used to silence LbNox, which resulted in decreased ROS signaling, limited formation of the Hartig net, and a lower mycorrhizal formation rate. Using Y2H library screening, BiFC and Co‐IP, we demonstrated an interaction between the mitogen‐activated protein kinase LbSakA and LbNoxR. LbSakA‐mediated phosphorylation of LbNoxR at T409, T477 and T480 positively modulates LbNox activity, ROS accumulation and upregulation of symbiosis‐related genes involved in dampening host defense reactions. These results demonstrate that regulation of fungal ROS metabolism is critical for maintaining the mutualistic interaction between L. bicolor and P. tremula × alba. Our findings also highlight a novel and complex regulatory mechanism governing the development of symbiosis, involving both transcriptional and posttranslational regulation of gene networks. 10.1111/nph.19813 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/nph.19813 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_nph_19813 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | LbSakA‐mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development Liang Shi Zi Wang Ju Hong Chen Hao Qiu Wei Dong Liu Xiao Yan Zhang Francis M. Martin Ming Wen Zhao New Phytologist LbSakA‐mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development Liang Shi Zi Wang Ju Hong Chen Hao Qiu Wei Dong Liu Xiao Yan Zhang Francis M. Martin Ming Wen Zhao New Phytologist Summary Ectomycorrhizal symbiosis, which involves mutually beneficial interactions between soil fungi and tree roots, is essential for promoting tree growth. To establish this symbiotic relationship, fungal symbionts must initiate and sustain mutualistic interactions with host plants while avoiding host defense responses. This study investigated the role of reactive oxygen species (ROS) generated by fungal NADPH oxidase (Nox) in the development of Laccaria bicolor/Populus tremula × alba symbiosis. Our findings revealed that L. bicolor LbNox expression was significantly higher in ectomycorrhizal roots than in free‐living mycelia. RNAi was used to silence LbNox, which resulted in decreased ROS signaling, limited formation of the Hartig net, and a lower mycorrhizal formation rate. Using Y2H library screening, BiFC and Co‐IP, we demonstrated an interaction between the mitogen‐activated protein kinase LbSakA and LbNoxR. LbSakA‐mediated phosphorylation of LbNoxR at T409, T477 and T480 positively modulates LbNox activity, ROS accumulation and upregulation of symbiosis‐related genes involved in dampening host defense reactions. These results demonstrate that regulation of fungal ROS metabolism is critical for maintaining the mutualistic interaction between L. bicolor and P. tremula × alba. Our findings also highlight a novel and complex regulatory mechanism governing the development of symbiosis, involving both transcriptional and posttranslational regulation of gene networks. 10.1111/nph.19813 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | LbSakA‐mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development |
| topic | New Phytologist |
| url | https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19813 |