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Autori principali: Zhonglai Luo, Spencer C. H. Barrett, Tieyao Tu, Zhongtao Zhao, Shanshan Jia, Shiran Gu, Tingting Duan, Yu Zhang, Bingqiang Xu, Lei Gu, Xiaofang Deng, Libo Jiang, Miaomiao Shi, Dianxiang Zhang
Natura: Artículo Open Access
Pubblicazione: Wiley 2025
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Accesso online:https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70521
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author Zhonglai Luo
Spencer C. H. Barrett
Tieyao Tu
Zhongtao Zhao
Shanshan Jia
Shiran Gu
Tingting Duan
Yu Zhang
Bingqiang Xu
Lei Gu
Xiaofang Deng
Libo Jiang
Miaomiao Shi
Dianxiang Zhang
author_facet Zhonglai Luo
Spencer C. H. Barrett
Tieyao Tu
Zhongtao Zhao
Shanshan Jia
Shiran Gu
Tingting Duan
Yu Zhang
Bingqiang Xu
Lei Gu
Xiaofang Deng
Libo Jiang
Miaomiao Shi
Dianxiang Zhang
Zhonglai Luo
Spencer C. H. Barrett
Tieyao Tu
Zhongtao Zhao
Shanshan Jia
Shiran Gu
Tingting Duan
Yu Zhang
Bingqiang Xu
Lei Gu
Xiaofang Deng
Libo Jiang
Miaomiao Shi
Dianxiang Zhang
collection Wiley Open Access
contents Genetic architecture of the S ‐locus supergene revealed in a tetraploid distylous species Zhonglai Luo Spencer C. H. Barrett Tieyao Tu Zhongtao Zhao Shanshan Jia Shiran Gu Tingting Duan Yu Zhang Bingqiang Xu Lei Gu Xiaofang Deng Libo Jiang Miaomiao Shi Dianxiang Zhang New Phytologist Summary Heterostyly is a polymorphic floral adaptation controlled by supergenes. The molecular basis of distyly has been investigated in diploid species from several unrelated families, but information is lacking for polyploid systems. Here, we address this knowledge gap in Schizomussaenda henryi , a tetraploid distylous species of Rubiaceae, the family with the greatest number of heterostylous species. Using chromosome‐level genome assemblies and transcriptome profiling, we characterized its tetraploid genome, identified the S ‐locus region, and performed evolutionary analysis. The S ‐locus contains four hemizygous genes in the S‐morph. SchzAUX22 emerged as a candidate gene potentially regulating both style length and filament growth via auxin signaling. Phylogenetic and k ‐mer‐based analysis suggested a hybrid allopolyploid origin for S. henryi , while no subgenome dominance was detected. Results from the comparison of K s values indicated that S ‐locus formation likely occurred through stepwise duplications. This study provides the first comprehensive genomic analysis of distyly in a polyploid species and demonstrates that the S ‐locus remains intact despite allopolyploidization resulting from hybridization. Our results indicated that polyploidization does not necessitate the breakdown of distyly, which occurs in several other heterostylous lineages. 10.1111/nph.70521 http://onlinelibrary.wiley.com/termsAndConditions#vor
doi_str_mv 10.1111/nph.70521
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institution Wiley Open Access
license_str_mv http://onlinelibrary.wiley.com/termsAndConditions#vor
publishDate 2025
publisher Wiley
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spellingShingle Genetic architecture of the S ‐locus supergene revealed in a tetraploid distylous species
Zhonglai Luo
Spencer C. H. Barrett
Tieyao Tu
Zhongtao Zhao
Shanshan Jia
Shiran Gu
Tingting Duan
Yu Zhang
Bingqiang Xu
Lei Gu
Xiaofang Deng
Libo Jiang
Miaomiao Shi
Dianxiang Zhang
New Phytologist
Genetic architecture of the S ‐locus supergene revealed in a tetraploid distylous species Zhonglai Luo Spencer C. H. Barrett Tieyao Tu Zhongtao Zhao Shanshan Jia Shiran Gu Tingting Duan Yu Zhang Bingqiang Xu Lei Gu Xiaofang Deng Libo Jiang Miaomiao Shi Dianxiang Zhang New Phytologist Summary Heterostyly is a polymorphic floral adaptation controlled by supergenes. The molecular basis of distyly has been investigated in diploid species from several unrelated families, but information is lacking for polyploid systems. Here, we address this knowledge gap in Schizomussaenda henryi , a tetraploid distylous species of Rubiaceae, the family with the greatest number of heterostylous species. Using chromosome‐level genome assemblies and transcriptome profiling, we characterized its tetraploid genome, identified the S ‐locus region, and performed evolutionary analysis. The S ‐locus contains four hemizygous genes in the S‐morph. SchzAUX22 emerged as a candidate gene potentially regulating both style length and filament growth via auxin signaling. Phylogenetic and k ‐mer‐based analysis suggested a hybrid allopolyploid origin for S. henryi , while no subgenome dominance was detected. Results from the comparison of K s values indicated that S ‐locus formation likely occurred through stepwise duplications. This study provides the first comprehensive genomic analysis of distyly in a polyploid species and demonstrates that the S ‐locus remains intact despite allopolyploidization resulting from hybridization. Our results indicated that polyploidization does not necessitate the breakdown of distyly, which occurs in several other heterostylous lineages. 10.1111/nph.70521 http://onlinelibrary.wiley.com/termsAndConditions#vor
title Genetic architecture of the S ‐locus supergene revealed in a tetraploid distylous species
topic New Phytologist
url https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70521