Arabidopsis phospholipid modifications mediate cellulase‐induced resistance to a fungal peptide antibiotic by imposing cell polarity
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| Formato: | Artículo Open Access |
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Wiley
2025
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| _version_ | 1867010132818264064 |
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| author | Saritha Panthapulakkal Narayanan Bradley R. Dotson Lise Noack Sanjana Holla Shichao Ren Peter Dörmann Susanne Widell Staffan Persson Ida Lager Allan G. Rasmusson |
| author_facet | Saritha Panthapulakkal Narayanan Bradley R. Dotson Lise Noack Sanjana Holla Shichao Ren Peter Dörmann Susanne Widell Staffan Persson Ida Lager Allan G. Rasmusson Saritha Panthapulakkal Narayanan Bradley R. Dotson Lise Noack Sanjana Holla Shichao Ren Peter Dörmann Susanne Widell Staffan Persson Ida Lager Allan G. Rasmusson |
| collection | Wiley Open Access |
| contents | Arabidopsis phospholipid modifications mediate cellulase‐induced resistance to a fungal peptide antibiotic by imposing cell polarity Saritha Panthapulakkal Narayanan Bradley R. Dotson Lise Noack Sanjana Holla Shichao Ren Peter Dörmann Susanne Widell Staffan Persson Ida Lager Allan G. Rasmusson New Phytologist Summary Plant‐symbiotic Trichoderma fungi attack microorganisms by secreting antibiotic membrane‐permeabilising peptaibols such as alamethicin. These peptaibols also permeabilise plant root epidermis plasma membranes (PMs), but mild pretreatment with Trichoderma cellulase activates a unique cellulase‐induced resistance to alamethicin (CIRA), via an unknown mechanism. We identify two Arabidopsis genes that are essential for the CIRA process: CIRA12 encodes a phosphatidylserine (PS) decarboxylase and CIRA13 , a phospholipase Dζ, implying that specific changes in anionic membrane lipids mediate alamethicin resistance. Fluorescent sensors revealed that cellulase induced a laterally asymmetric decrease in PS and surface charge to outer periclinal root epidermal PMs. Consistently, the CIRA response was reversed by addition of lysoPS. CIRA13 is essential for vesicle trafficking, which in turn is crucial for CIRA induction. Overall, cellulase induces a cellular polarity with respect to phospholipids, not previously observed in plants, that is leading to increased lipid packing and preventing peptaibol permeabilization of the outer periclinal membrane. 10.1111/nph.70721 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1111/nph.70721 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_nph_70721 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Arabidopsis phospholipid modifications mediate cellulase‐induced resistance to a fungal peptide antibiotic by imposing cell polarity Saritha Panthapulakkal Narayanan Bradley R. Dotson Lise Noack Sanjana Holla Shichao Ren Peter Dörmann Susanne Widell Staffan Persson Ida Lager Allan G. Rasmusson New Phytologist Arabidopsis phospholipid modifications mediate cellulase‐induced resistance to a fungal peptide antibiotic by imposing cell polarity Saritha Panthapulakkal Narayanan Bradley R. Dotson Lise Noack Sanjana Holla Shichao Ren Peter Dörmann Susanne Widell Staffan Persson Ida Lager Allan G. Rasmusson New Phytologist Summary Plant‐symbiotic Trichoderma fungi attack microorganisms by secreting antibiotic membrane‐permeabilising peptaibols such as alamethicin. These peptaibols also permeabilise plant root epidermis plasma membranes (PMs), but mild pretreatment with Trichoderma cellulase activates a unique cellulase‐induced resistance to alamethicin (CIRA), via an unknown mechanism. We identify two Arabidopsis genes that are essential for the CIRA process: CIRA12 encodes a phosphatidylserine (PS) decarboxylase and CIRA13 , a phospholipase Dζ, implying that specific changes in anionic membrane lipids mediate alamethicin resistance. Fluorescent sensors revealed that cellulase induced a laterally asymmetric decrease in PS and surface charge to outer periclinal root epidermal PMs. Consistently, the CIRA response was reversed by addition of lysoPS. CIRA13 is essential for vesicle trafficking, which in turn is crucial for CIRA induction. Overall, cellulase induces a cellular polarity with respect to phospholipids, not previously observed in plants, that is leading to increased lipid packing and preventing peptaibol permeabilization of the outer periclinal membrane. 10.1111/nph.70721 http://creativecommons.org/licenses/by/4.0/ |
| title | Arabidopsis phospholipid modifications mediate cellulase‐induced resistance to a fungal peptide antibiotic by imposing cell polarity |
| topic | New Phytologist |
| url | https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70721 |