HOXC 6 Activates BCAT 1 Expression to Promote Oral Cancer Development
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| Formato: | Artículo Open Access |
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Wiley
2025
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| _version_ | 1867016714717233152 |
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| author | Meiheriban Tuerhong Zaynure Wubulihasimu Bo Xu Ling Zhang |
| author_facet | Meiheriban Tuerhong Zaynure Wubulihasimu Bo Xu Ling Zhang Meiheriban Tuerhong Zaynure Wubulihasimu Bo Xu Ling Zhang |
| collection | Wiley Open Access |
| contents | HOXC 6 Activates BCAT 1 Expression to Promote Oral Cancer Development Meiheriban Tuerhong Zaynure Wubulihasimu Bo Xu Ling Zhang Oral Diseases ABSTRACT Objective This paper aims to reveal the impact of HOXC6 on oral cancer development through the regulation of BCAT1 and the molecular mechanism. Methods BCAT1 expression in oral cancer tissues and adjacent tissues was verified by western blot analysis and RT‐qPCR. After knocking down BCAT1, the HN6 and HN30 cell proliferation, migration, invasion, and apoptosis were detected. The upstream mechanism of BCAT1 elevation was investigated by bioinformatics analysis. HOXC6 expression was detected by immunohistochemistry in oral cancer and adjacent tissues. ChIP and dual‐luciferase assay were used to detect the interaction between HOXC6 and the BCAT1 promoter. Rescue experiments were carried out to substantiate the malignant phenotype and epithelial‐to‐mesenchymal transition (EMT) of oral cancer cells. An in vivo xenograft tumor model was constructed. Results BCAT1 and HOXC6 were abnormally high in oral cancer tissues. Inhibition of BCAT1 suppressed the proliferation, migration, invasion, and EMT, and promoted apoptosis of oral cancer cells. HOXC6 downregulation curbed the malignant behavior and EMT program of oral cancer cells. HOXC6 was enriched in the BCAT1 promoter in oral cancer cells. HOXC6 silencing downregulated BCAT1 expression. Transcriptional activation of BCAT1 by HOXC6 promoted oral cancer progression. Conclusion HOXC6 knockdown inhibits BCAT1 expression to suppress oral cancer development. 10.1111/odi.70049 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/odi.70049 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_odi_70049 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | HOXC 6 Activates BCAT 1 Expression to Promote Oral Cancer Development Meiheriban Tuerhong Zaynure Wubulihasimu Bo Xu Ling Zhang Oral Diseases HOXC 6 Activates BCAT 1 Expression to Promote Oral Cancer Development Meiheriban Tuerhong Zaynure Wubulihasimu Bo Xu Ling Zhang Oral Diseases ABSTRACT Objective This paper aims to reveal the impact of HOXC6 on oral cancer development through the regulation of BCAT1 and the molecular mechanism. Methods BCAT1 expression in oral cancer tissues and adjacent tissues was verified by western blot analysis and RT‐qPCR. After knocking down BCAT1, the HN6 and HN30 cell proliferation, migration, invasion, and apoptosis were detected. The upstream mechanism of BCAT1 elevation was investigated by bioinformatics analysis. HOXC6 expression was detected by immunohistochemistry in oral cancer and adjacent tissues. ChIP and dual‐luciferase assay were used to detect the interaction between HOXC6 and the BCAT1 promoter. Rescue experiments were carried out to substantiate the malignant phenotype and epithelial‐to‐mesenchymal transition (EMT) of oral cancer cells. An in vivo xenograft tumor model was constructed. Results BCAT1 and HOXC6 were abnormally high in oral cancer tissues. Inhibition of BCAT1 suppressed the proliferation, migration, invasion, and EMT, and promoted apoptosis of oral cancer cells. HOXC6 downregulation curbed the malignant behavior and EMT program of oral cancer cells. HOXC6 was enriched in the BCAT1 promoter in oral cancer cells. HOXC6 silencing downregulated BCAT1 expression. Transcriptional activation of BCAT1 by HOXC6 promoted oral cancer progression. Conclusion HOXC6 knockdown inhibits BCAT1 expression to suppress oral cancer development. 10.1111/odi.70049 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | HOXC 6 Activates BCAT 1 Expression to Promote Oral Cancer Development |
| topic | Oral Diseases |
| url | https://onlinelibrary.wiley.com/doi/10.1111/odi.70049 |