Inhibitor of cardiolipin biosynthesis‐related enzyme MoGep4 confers broad‐spectrum anti‐fungal activity
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| Format: | Artículo Open Access |
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Wiley
2024
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| author | Peng Sun Juan Zhao Gan Sha Yaru Zhou Mengfei Zhao Renjian Li Xiaojing Kong Qiping Sun Yun Li Ke Li Ruiqing Bi Lei Yang Ziting Qin Wenzheng Huang Yin Wang Jie Gao Guang Chen Haifeng Zhang Muhammad Adnan Long Yang Lu Zheng Xiao‐Lin Chen Guanghui Wang Toshiki Ishikawa Qiang Li Jin‐Rong Xu Guotian Li |
| author_facet | Peng Sun Juan Zhao Gan Sha Yaru Zhou Mengfei Zhao Renjian Li Xiaojing Kong Qiping Sun Yun Li Ke Li Ruiqing Bi Lei Yang Ziting Qin Wenzheng Huang Yin Wang Jie Gao Guang Chen Haifeng Zhang Muhammad Adnan Long Yang Lu Zheng Xiao‐Lin Chen Guanghui Wang Toshiki Ishikawa Qiang Li Jin‐Rong Xu Guotian Li Peng Sun Juan Zhao Gan Sha Yaru Zhou Mengfei Zhao Renjian Li Xiaojing Kong Qiping Sun Yun Li Ke Li Ruiqing Bi Lei Yang Ziting Qin Wenzheng Huang Yin Wang Jie Gao Guang Chen Haifeng Zhang Muhammad Adnan Long Yang Lu Zheng Xiao‐Lin Chen Guanghui Wang Toshiki Ishikawa Qiang Li Jin‐Rong Xu Guotian Li |
| collection | Wiley Open Access |
| contents | Inhibitor of cardiolipin biosynthesis‐related enzyme MoGep4 confers broad‐spectrum anti‐fungal activity Peng Sun Juan Zhao Gan Sha Yaru Zhou Mengfei Zhao Renjian Li Xiaojing Kong Qiping Sun Yun Li Ke Li Ruiqing Bi Lei Yang Ziting Qin Wenzheng Huang Yin Wang Jie Gao Guang Chen Haifeng Zhang Muhammad Adnan Long Yang Lu Zheng Xiao‐Lin Chen Guanghui Wang Toshiki Ishikawa Qiang Li Jin‐Rong Xu Guotian Li Plant, Cell & Environment Abstract Plant pathogens cause devastating diseases, leading to serious losses to agriculture. Mechanistic understanding of pathogenesis of plant pathogens lays the foundation for the development of fungicides for disease control. Mitophagy, a specific form of autophagy, is important for fungal virulence. The role of cardiolipin, mitochondrial signature phospholipid, in mitophagy and pathogenesis is largely unknown in plant pathogenic fungi. The functions of enzymes involved in cardiolipin biosynthesis and relevant inhibitors were assessed using a set of assays, including genetic deletion, plant infection, lipidomics, chemical‐protein interaction, chemical inhibition, and field trials. Our results showed that the cardiolipin biosynthesis‐related gene MoGEP4 of the rice blast fungus Magnaporthe oryzae regulates growth, conidiation, cardiolipin biosynthesis, and virulence. Mechanistically, MoGep4 regulated mitophagy and Mps1‐MAPK phosphorylation, which are required for virulence. Chemical alexidine dihydrochloride (AXD) inhibited the enzyme activity of MoGep4, cardiolipin biosynthesis and mitophagy. Importantly, AXD efficiently inhibited the growth of 10 plant pathogens and controlled rice blast and Fusarium head blight in the field. Our study demonstrated that MoGep4 regulates mitophagy, Mps1 phosphorylation and pathogenesis in M. oryzae . In addition, we found that the MoGep4 inhibitor, AXD, displays broad‐spectrum antifungal activity and is a promising candidate for fungicide development. 10.1111/pce.15021 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/pce.15021 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_pce_15021 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Inhibitor of cardiolipin biosynthesis‐related enzyme MoGep4 confers broad‐spectrum anti‐fungal activity Peng Sun Juan Zhao Gan Sha Yaru Zhou Mengfei Zhao Renjian Li Xiaojing Kong Qiping Sun Yun Li Ke Li Ruiqing Bi Lei Yang Ziting Qin Wenzheng Huang Yin Wang Jie Gao Guang Chen Haifeng Zhang Muhammad Adnan Long Yang Lu Zheng Xiao‐Lin Chen Guanghui Wang Toshiki Ishikawa Qiang Li Jin‐Rong Xu Guotian Li Plant, Cell & Environment Inhibitor of cardiolipin biosynthesis‐related enzyme MoGep4 confers broad‐spectrum anti‐fungal activity Peng Sun Juan Zhao Gan Sha Yaru Zhou Mengfei Zhao Renjian Li Xiaojing Kong Qiping Sun Yun Li Ke Li Ruiqing Bi Lei Yang Ziting Qin Wenzheng Huang Yin Wang Jie Gao Guang Chen Haifeng Zhang Muhammad Adnan Long Yang Lu Zheng Xiao‐Lin Chen Guanghui Wang Toshiki Ishikawa Qiang Li Jin‐Rong Xu Guotian Li Plant, Cell & Environment Abstract Plant pathogens cause devastating diseases, leading to serious losses to agriculture. Mechanistic understanding of pathogenesis of plant pathogens lays the foundation for the development of fungicides for disease control. Mitophagy, a specific form of autophagy, is important for fungal virulence. The role of cardiolipin, mitochondrial signature phospholipid, in mitophagy and pathogenesis is largely unknown in plant pathogenic fungi. The functions of enzymes involved in cardiolipin biosynthesis and relevant inhibitors were assessed using a set of assays, including genetic deletion, plant infection, lipidomics, chemical‐protein interaction, chemical inhibition, and field trials. Our results showed that the cardiolipin biosynthesis‐related gene MoGEP4 of the rice blast fungus Magnaporthe oryzae regulates growth, conidiation, cardiolipin biosynthesis, and virulence. Mechanistically, MoGep4 regulated mitophagy and Mps1‐MAPK phosphorylation, which are required for virulence. Chemical alexidine dihydrochloride (AXD) inhibited the enzyme activity of MoGep4, cardiolipin biosynthesis and mitophagy. Importantly, AXD efficiently inhibited the growth of 10 plant pathogens and controlled rice blast and Fusarium head blight in the field. Our study demonstrated that MoGep4 regulates mitophagy, Mps1 phosphorylation and pathogenesis in M. oryzae . In addition, we found that the MoGep4 inhibitor, AXD, displays broad‐spectrum antifungal activity and is a promising candidate for fungicide development. 10.1111/pce.15021 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Inhibitor of cardiolipin biosynthesis‐related enzyme MoGep4 confers broad‐spectrum anti‐fungal activity |
| topic | Plant, Cell & Environment |
| url | https://onlinelibrary.wiley.com/doi/10.1111/pce.15021 |