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| Format: | Artículo Open Access |
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Wiley
2024
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| Accès en ligne: | https://onlinelibrary.wiley.com/doi/10.1111/php.13905 |
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| author | Amir Hossein Safari Saeed Sadat Mansouri Babak Iranpour Mahshid Hodjat Neda Hakimiha |
| author_facet | Amir Hossein Safari Saeed Sadat Mansouri Babak Iranpour Mahshid Hodjat Neda Hakimiha Amir Hossein Safari Saeed Sadat Mansouri Babak Iranpour Mahshid Hodjat Neda Hakimiha |
| collection | Wiley Open Access |
| contents | An in vitro study on the effects of photobiomodulation by diode lasers (red, infrared, and red–infrared combination) on periodontal ligament mesenchymal stem cells treated with bisphosphonates Amir Hossein Safari Saeed Sadat Mansouri Babak Iranpour Mahshid Hodjat Neda Hakimiha Photochemistry and Photobiology AbstractThis study evaluated the effect of photobiomodulation therapy (PBMT) using 660 and 808 nm diode lasers (individual and in combination) on periodontal ligament mesenchymal stem cells (PDLSCs) in the presence of zoledronic acid (ZA). PDLSCs were cultured for 48 h in DMEM complete medium containing 5 μM ZA. PBMT was done three times with a 24‐h interval in groups 1 (660 nm, 5 J/cm2), 2 (880 nm, 3 J/cm2), and 3 (660 + 808 nm) either in normal or ZA‐treated culture medium. Control groups did not receive PBMT. Twenty‐four hours post‐irradiation, cell proliferation and expression of RANKL and OPG were assessed using MTT and real‐time PCR tests, respectively. The results showed a significant decrease in cell viability in ZA‐treated cells (p < 0.001). Additionally, ZA induced the expression of OPG (p = 0.03) while reducing RANKL (p < 0.001). Cell proliferation was significantly increased in 808 and 660 + 808 nm groups. Moreover, all PBMT groups could significantly increase and decrease the RANKL and OPG, respectively, in the presence of ZA (all p < 0.001). A combination of 660 + 808 nm showed the highest effects on both genes. In conclusion, it seems that PBMT can modulate the effects of ZA by inducing PDLSC proliferation and increasing RANKL‐to‐OPG gene expression ratio. 10.1111/php.13905 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/php.13905 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_php_13905 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | An in vitro study on the effects of photobiomodulation by diode lasers (red, infrared, and red–infrared combination) on periodontal ligament mesenchymal stem cells treated with bisphosphonates Amir Hossein Safari Saeed Sadat Mansouri Babak Iranpour Mahshid Hodjat Neda Hakimiha Photochemistry and Photobiology An in vitro study on the effects of photobiomodulation by diode lasers (red, infrared, and red–infrared combination) on periodontal ligament mesenchymal stem cells treated with bisphosphonates Amir Hossein Safari Saeed Sadat Mansouri Babak Iranpour Mahshid Hodjat Neda Hakimiha Photochemistry and Photobiology AbstractThis study evaluated the effect of photobiomodulation therapy (PBMT) using 660 and 808 nm diode lasers (individual and in combination) on periodontal ligament mesenchymal stem cells (PDLSCs) in the presence of zoledronic acid (ZA). PDLSCs were cultured for 48 h in DMEM complete medium containing 5 μM ZA. PBMT was done three times with a 24‐h interval in groups 1 (660 nm, 5 J/cm2), 2 (880 nm, 3 J/cm2), and 3 (660 + 808 nm) either in normal or ZA‐treated culture medium. Control groups did not receive PBMT. Twenty‐four hours post‐irradiation, cell proliferation and expression of RANKL and OPG were assessed using MTT and real‐time PCR tests, respectively. The results showed a significant decrease in cell viability in ZA‐treated cells (p < 0.001). Additionally, ZA induced the expression of OPG (p = 0.03) while reducing RANKL (p < 0.001). Cell proliferation was significantly increased in 808 and 660 + 808 nm groups. Moreover, all PBMT groups could significantly increase and decrease the RANKL and OPG, respectively, in the presence of ZA (all p < 0.001). A combination of 660 + 808 nm showed the highest effects on both genes. In conclusion, it seems that PBMT can modulate the effects of ZA by inducing PDLSC proliferation and increasing RANKL‐to‐OPG gene expression ratio. 10.1111/php.13905 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | An in vitro study on the effects of photobiomodulation by diode lasers (red, infrared, and red–infrared combination) on periodontal ligament mesenchymal stem cells treated with bisphosphonates |
| topic | Photochemistry and Photobiology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/php.13905 |