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| Main Authors: | , , , , , , , , |
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| Format: | Artículo Open Access |
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Wiley
2024
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| Online Access: | https://onlinelibrary.wiley.com/doi/10.1111/php.13944 |
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| author | João Vitor de Oliveira Silva Jean Eduardo Meneguello Maíra Dante Formagio Camila Fabiano de Freitas Luis Carlos Malacarne Rogério Marchiosi Patrícia de Souza Bonfim de Mendonça Paula Aline Zanetti Campanerut‐Sá Jane Martha Graton Mikcha |
| author_facet | João Vitor de Oliveira Silva Jean Eduardo Meneguello Maíra Dante Formagio Camila Fabiano de Freitas Luis Carlos Malacarne Rogério Marchiosi Patrícia de Souza Bonfim de Mendonça Paula Aline Zanetti Campanerut‐Sá Jane Martha Graton Mikcha João Vitor de Oliveira Silva Jean Eduardo Meneguello Maíra Dante Formagio Camila Fabiano de Freitas Luis Carlos Malacarne Rogério Marchiosi Patrícia de Souza Bonfim de Mendonça Paula Aline Zanetti Campanerut‐Sá Jane Martha Graton Mikcha |
| collection | Wiley Open Access |
| contents | Multi‐targets of antimicrobial photodynamic therapy mediated by erythrosine against Staphylococcus aureus identified by proteomic approach João Vitor de Oliveira Silva Jean Eduardo Meneguello Maíra Dante Formagio Camila Fabiano de Freitas Luis Carlos Malacarne Rogério Marchiosi Patrícia de Souza Bonfim de Mendonça Paula Aline Zanetti Campanerut‐Sá Jane Martha Graton Mikcha Photochemistry and Photobiology AbstractStaphylococcus aureus is a global challenge to the clinical field and food industry. Therefore, the development of antimicrobial photodynamic therapy (aPDT) has become one of the valuable methods to control this pathogen. The antibacterial activity of photoinactivation by erythrosine (Ery) against S. aureus has been reported, but its modes of action are unclear. This study aimed to employ a proteomic approach to analyze modes of action of Ery‐aPDT against S. aureus. We determined the antibacterial effect by Ery‐aPDT assays, quantified reactive oxygen species (ROS) and injury to the cell membrane, and determined protein expression using a proteomic approach combined with bioinformatic tools. Ery‐aPDT was effective in reducing S. aureus to undetectable levels. In addition, the increment of ROS accompanied the increase in the reduction of cell viability, and damage to cellular membranes was shown by sublethal injury. In proteomic analysis, we found 17 differentially expressed proteins. These proteins revealed changes mainly associated with defense to oxidative stress, energy metabolism, translation, and protein biosynthesis. Thus, these results suggest that the effectiveness of Ery‐aPDT is due to multi‐targets in the bacterial cell that cause the death of S. aureus. 10.1111/php.13944 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/php.13944 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_php_13944 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Multi‐targets of antimicrobial photodynamic therapy mediated by erythrosine against Staphylococcus aureus identified by proteomic approach João Vitor de Oliveira Silva Jean Eduardo Meneguello Maíra Dante Formagio Camila Fabiano de Freitas Luis Carlos Malacarne Rogério Marchiosi Patrícia de Souza Bonfim de Mendonça Paula Aline Zanetti Campanerut‐Sá Jane Martha Graton Mikcha Photochemistry and Photobiology Multi‐targets of antimicrobial photodynamic therapy mediated by erythrosine against Staphylococcus aureus identified by proteomic approach João Vitor de Oliveira Silva Jean Eduardo Meneguello Maíra Dante Formagio Camila Fabiano de Freitas Luis Carlos Malacarne Rogério Marchiosi Patrícia de Souza Bonfim de Mendonça Paula Aline Zanetti Campanerut‐Sá Jane Martha Graton Mikcha Photochemistry and Photobiology AbstractStaphylococcus aureus is a global challenge to the clinical field and food industry. Therefore, the development of antimicrobial photodynamic therapy (aPDT) has become one of the valuable methods to control this pathogen. The antibacterial activity of photoinactivation by erythrosine (Ery) against S. aureus has been reported, but its modes of action are unclear. This study aimed to employ a proteomic approach to analyze modes of action of Ery‐aPDT against S. aureus. We determined the antibacterial effect by Ery‐aPDT assays, quantified reactive oxygen species (ROS) and injury to the cell membrane, and determined protein expression using a proteomic approach combined with bioinformatic tools. Ery‐aPDT was effective in reducing S. aureus to undetectable levels. In addition, the increment of ROS accompanied the increase in the reduction of cell viability, and damage to cellular membranes was shown by sublethal injury. In proteomic analysis, we found 17 differentially expressed proteins. These proteins revealed changes mainly associated with defense to oxidative stress, energy metabolism, translation, and protein biosynthesis. Thus, these results suggest that the effectiveness of Ery‐aPDT is due to multi‐targets in the bacterial cell that cause the death of S. aureus. 10.1111/php.13944 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Multi‐targets of antimicrobial photodynamic therapy mediated by erythrosine against Staphylococcus aureus identified by proteomic approach |
| topic | Photochemistry and Photobiology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/php.13944 |