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| Main Authors: | , , , , , , |
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| Format: | Artículo Open Access |
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Wiley
2024
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| Online Access: | https://onlinelibrary.wiley.com/doi/10.1111/php.13995 |
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| _version_ | 1867015194269450240 |
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| author | Shin‐ichi Sasaki Yuto Morioka Kohta Maegawa Yuya Katsuragi Takashi Nakamura Kazuo Kamemura Hitoshi Tamiaki |
| author_facet | Shin‐ichi Sasaki Yuto Morioka Kohta Maegawa Yuya Katsuragi Takashi Nakamura Kazuo Kamemura Hitoshi Tamiaki Shin‐ichi Sasaki Yuto Morioka Kohta Maegawa Yuya Katsuragi Takashi Nakamura Kazuo Kamemura Hitoshi Tamiaki |
| collection | Wiley Open Access |
| contents | Pyrobacteriopheophorbide‐a derivatives possessing various hydrophilic esterifying groups at the C17‐propionate residues for photodynamic therapy Shin‐ichi Sasaki Yuto Morioka Kohta Maegawa Yuya Katsuragi Takashi Nakamura Kazuo Kamemura Hitoshi Tamiaki Photochemistry and Photobiology AbstractAiming at the application to photodynamic therapy, natural bacteriochlorophyll‐a was converted to chemically stable free‐base derivatives possessing different kinds of hydrophilic C17‐propionate residues. These semi‐synthetic bacteriochlorins were found to have self‐assembling ability in an aqueous environment and formed stable J‐type aggregates in a cell culture medium containing 0.2% DMSO. The electronic absorption spectra of all the sensitizers showed Qy absorption maxima at 754 nm in DMSO as their monomeric states, while a drastic shift of the red‐most bands to ca. 880 nm was observed in the aqueous medium. The circular dichroism spectra in the medium showed much intense signals compared to those measured in DMSO, supporting the formation of well‐ordered supramolecular structures. By introducing hydrophilic side chains, the bacteriochlorin sensitizers could be dispersed in the aqueous medium as their J‐aggregates without the use of any surfactants. Cellular uptake efficiencies as well as photodynamic activities were evaluated using human cervical adenocarcinoma HeLa cells. Among the 11 photosensitizers investigated, the best result was obtained for a charged derivative possessing trimethylammonium terminal (17‐CH2CH2COOCH2CH2N+(CH3)3I−) and photocytotoxicity of EC50 = 0.09 μM was achieved by far‐red light illumination of 35 J/cm2 from an LED panel (730 nm). 10.1111/php.13995 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/php.13995 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_php_13995 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Pyrobacteriopheophorbide‐a derivatives possessing various hydrophilic esterifying groups at the C17‐propionate residues for photodynamic therapy Shin‐ichi Sasaki Yuto Morioka Kohta Maegawa Yuya Katsuragi Takashi Nakamura Kazuo Kamemura Hitoshi Tamiaki Photochemistry and Photobiology Pyrobacteriopheophorbide‐a derivatives possessing various hydrophilic esterifying groups at the C17‐propionate residues for photodynamic therapy Shin‐ichi Sasaki Yuto Morioka Kohta Maegawa Yuya Katsuragi Takashi Nakamura Kazuo Kamemura Hitoshi Tamiaki Photochemistry and Photobiology AbstractAiming at the application to photodynamic therapy, natural bacteriochlorophyll‐a was converted to chemically stable free‐base derivatives possessing different kinds of hydrophilic C17‐propionate residues. These semi‐synthetic bacteriochlorins were found to have self‐assembling ability in an aqueous environment and formed stable J‐type aggregates in a cell culture medium containing 0.2% DMSO. The electronic absorption spectra of all the sensitizers showed Qy absorption maxima at 754 nm in DMSO as their monomeric states, while a drastic shift of the red‐most bands to ca. 880 nm was observed in the aqueous medium. The circular dichroism spectra in the medium showed much intense signals compared to those measured in DMSO, supporting the formation of well‐ordered supramolecular structures. By introducing hydrophilic side chains, the bacteriochlorin sensitizers could be dispersed in the aqueous medium as their J‐aggregates without the use of any surfactants. Cellular uptake efficiencies as well as photodynamic activities were evaluated using human cervical adenocarcinoma HeLa cells. Among the 11 photosensitizers investigated, the best result was obtained for a charged derivative possessing trimethylammonium terminal (17‐CH2CH2COOCH2CH2N+(CH3)3I−) and photocytotoxicity of EC50 = 0.09 μM was achieved by far‐red light illumination of 35 J/cm2 from an LED panel (730 nm). 10.1111/php.13995 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Pyrobacteriopheophorbide‐a derivatives possessing various hydrophilic esterifying groups at the C17‐propionate residues for photodynamic therapy |
| topic | Photochemistry and Photobiology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/php.13995 |