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| Main Authors: | , , , , , |
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| Format: | Artículo Open Access |
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Wiley
2024
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| Online Access: | https://onlinelibrary.wiley.com/doi/10.1111/php.14017 |
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| _version_ | 1867018397350363136 |
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| author | Luana B. Trentin Altevir R. Viana Sophia Iwersen Bernardo A. Iglesias Otávio A. Chaves André P. Schuch |
| author_facet | Luana B. Trentin Altevir R. Viana Sophia Iwersen Bernardo A. Iglesias Otávio A. Chaves André P. Schuch Luana B. Trentin Altevir R. Viana Sophia Iwersen Bernardo A. Iglesias Otávio A. Chaves André P. Schuch |
| collection | Wiley Open Access |
| contents | Light exposure of tetra‐cationic porphyrins containing peripheral Pd(II)‐bipyridyl complexes and the induced effects on purified DNA molecule, fibroblast and melanoma cell lines Luana B. Trentin Altevir R. Viana Sophia Iwersen Bernardo A. Iglesias Otávio A. Chaves André P. Schuch Photochemistry and Photobiology AbstractPhotodynamic therapy (PDT) combines a light source, oxygen, and a photosensitizer (PS) to generate reactive oxygen species (ROS) for treating diseases. In this study, we evaluated two meso‐tetra‐pyridyl porphyrins with [Pd(bpy)Cl]+, namely 3‐PdTPyP and 4‐PdTPyP, as PS for PDT application. DNA interaction was assessed by spectroscopic measurements (UV–Vis and fluorescence emission), viscosity analysis, and molecular docking simulations. The results indicate that Pd(II)‐porphyrins do not intercalate into DNA, suggesting that the minor groove is the primary interaction site, mainly through van der Waals forces. These metalloporphyrins effectively induced nitrogenous bases oxidation, particularly in purines, after white light irradiation. The induced DNA lesions were able to inactivate plasmid DNA metabolism (DNA replication and transcription) in a bacterial model. 3‐PdTPyP and 4‐PdTPyP significantly decreased the viability of treated melanoma cell lines (A375 and B16‐F10), demonstrating that melanoma cell lines were more sensitive to these Pd(II)‐porphyrins than the fibroblast cell line (L929). Moreover, 3‐PdTPyP was more photototoxic to A375 cells (IC50 = 0.43 μM), whereas 4‐PdTPyP was more photototoxic to B16‐F10 cells (IC50 = 0.51 μM). These findings suggest that these porphyrins are promising PS for future PDT research focused on skin cancer. 10.1111/php.14017 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/php.14017 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_php_14017 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Light exposure of tetra‐cationic porphyrins containing peripheral Pd(II)‐bipyridyl complexes and the induced effects on purified DNA molecule, fibroblast and melanoma cell lines Luana B. Trentin Altevir R. Viana Sophia Iwersen Bernardo A. Iglesias Otávio A. Chaves André P. Schuch Photochemistry and Photobiology Light exposure of tetra‐cationic porphyrins containing peripheral Pd(II)‐bipyridyl complexes and the induced effects on purified DNA molecule, fibroblast and melanoma cell lines Luana B. Trentin Altevir R. Viana Sophia Iwersen Bernardo A. Iglesias Otávio A. Chaves André P. Schuch Photochemistry and Photobiology AbstractPhotodynamic therapy (PDT) combines a light source, oxygen, and a photosensitizer (PS) to generate reactive oxygen species (ROS) for treating diseases. In this study, we evaluated two meso‐tetra‐pyridyl porphyrins with [Pd(bpy)Cl]+, namely 3‐PdTPyP and 4‐PdTPyP, as PS for PDT application. DNA interaction was assessed by spectroscopic measurements (UV–Vis and fluorescence emission), viscosity analysis, and molecular docking simulations. The results indicate that Pd(II)‐porphyrins do not intercalate into DNA, suggesting that the minor groove is the primary interaction site, mainly through van der Waals forces. These metalloporphyrins effectively induced nitrogenous bases oxidation, particularly in purines, after white light irradiation. The induced DNA lesions were able to inactivate plasmid DNA metabolism (DNA replication and transcription) in a bacterial model. 3‐PdTPyP and 4‐PdTPyP significantly decreased the viability of treated melanoma cell lines (A375 and B16‐F10), demonstrating that melanoma cell lines were more sensitive to these Pd(II)‐porphyrins than the fibroblast cell line (L929). Moreover, 3‐PdTPyP was more photototoxic to A375 cells (IC50 = 0.43 μM), whereas 4‐PdTPyP was more photototoxic to B16‐F10 cells (IC50 = 0.51 μM). These findings suggest that these porphyrins are promising PS for future PDT research focused on skin cancer. 10.1111/php.14017 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Light exposure of tetra‐cationic porphyrins containing peripheral Pd(II)‐bipyridyl complexes and the induced effects on purified DNA molecule, fibroblast and melanoma cell lines |
| topic | Photochemistry and Photobiology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/php.14017 |