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| Format: | Artículo Open Access |
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Wiley
2024
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| Online Access: | https://onlinelibrary.wiley.com/doi/10.1111/php.14019 |
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| author | Keyvan Khosh Abady Negar Karpourazar Arjun Krishnamoorthi Runze Li Peter M. Rentzepis |
| author_facet | Keyvan Khosh Abady Negar Karpourazar Arjun Krishnamoorthi Runze Li Peter M. Rentzepis Keyvan Khosh Abady Negar Karpourazar Arjun Krishnamoorthi Runze Li Peter M. Rentzepis |
| collection | Wiley Open Access |
| contents | Spectroscopic analysis of bacterial photoreactivation Keyvan Khosh Abady Negar Karpourazar Arjun Krishnamoorthi Runze Li Peter M. Rentzepis Photochemistry and Photobiology AbstractWith the rise of bacterial infections and antibiotic resistance, spectroscopic devices originally developed for bacterial detection have shown promise to rapidly identify bacterial strains and determine the ratio of live to dead bacteria. However, the detection of the photoreactivated pathogens remains a critical concern. This study utilizes fluorescence and Raman spectroscopy to analyze bacterial responses to UV irradiation and subsequent photoreactivation. Our experimental results reveal limitations in fluorescence spectroscopy for detecting photoreactivated bacteria, as the intense fluorescence of tryptophan and tyrosine amino acids masks the fluorescence emitted by thymine molecules. Conversely, Raman spectroscopy proves more effective, showing a detectable decrease in band intensities of E. coli bacteria at 1248 and 1665 cm−1 after exposure to UVC radiation. Subsequent UVA irradiation results in the partial restoration of these band intensities, indicating DNA repair and bacterial photoreactivation. This enhanced understanding aims to improve the accuracy and effectiveness of these spectroscopic tools in clinical and environmental settings. 10.1111/php.14019 http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| doi_str_mv | 10.1111/php.14019 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_php_14019 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Spectroscopic analysis of bacterial photoreactivation Keyvan Khosh Abady Negar Karpourazar Arjun Krishnamoorthi Runze Li Peter M. Rentzepis Photochemistry and Photobiology Spectroscopic analysis of bacterial photoreactivation Keyvan Khosh Abady Negar Karpourazar Arjun Krishnamoorthi Runze Li Peter M. Rentzepis Photochemistry and Photobiology AbstractWith the rise of bacterial infections and antibiotic resistance, spectroscopic devices originally developed for bacterial detection have shown promise to rapidly identify bacterial strains and determine the ratio of live to dead bacteria. However, the detection of the photoreactivated pathogens remains a critical concern. This study utilizes fluorescence and Raman spectroscopy to analyze bacterial responses to UV irradiation and subsequent photoreactivation. Our experimental results reveal limitations in fluorescence spectroscopy for detecting photoreactivated bacteria, as the intense fluorescence of tryptophan and tyrosine amino acids masks the fluorescence emitted by thymine molecules. Conversely, Raman spectroscopy proves more effective, showing a detectable decrease in band intensities of E. coli bacteria at 1248 and 1665 cm−1 after exposure to UVC radiation. Subsequent UVA irradiation results in the partial restoration of these band intensities, indicating DNA repair and bacterial photoreactivation. This enhanced understanding aims to improve the accuracy and effectiveness of these spectroscopic tools in clinical and environmental settings. 10.1111/php.14019 http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| title | Spectroscopic analysis of bacterial photoreactivation |
| topic | Photochemistry and Photobiology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/php.14019 |