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| Autores principales: | , , , , , , |
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| Formato: | Artículo Open Access |
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Wiley
2025
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| Acceso en línea: | https://onlinelibrary.wiley.com/doi/10.1111/php.70051 |
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| _version_ | 1867007179840552960 |
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| author | Maria Sinetova Nina Spohrer David Gabrielyan Luke Lehmann Julian Breinich Dmitry Los Tilman Lamparter |
| author_facet | Maria Sinetova Nina Spohrer David Gabrielyan Luke Lehmann Julian Breinich Dmitry Los Tilman Lamparter Maria Sinetova Nina Spohrer David Gabrielyan Luke Lehmann Julian Breinich Dmitry Los Tilman Lamparter |
| collection | Wiley Open Access |
| contents | Photophobotaxis of single‐celled and filamentous cyanobacteria Maria Sinetova Nina Spohrer David Gabrielyan Luke Lehmann Julian Breinich Dmitry Los Tilman Lamparter Photochemistry and Photobiology Abstract Phototaxis refers to an organism's movement toward a light source, while photophobotaxis involves movement into illuminated regions. Although phototaxis in cyanobacteria has been widely studied, photophobotaxis has been investigated in only a few species. In this study, we examined photophobotaxis of 7 single‐celled and 11 filamentous cyanobacterial species, among them 3 Nostocales (filaments with heterocysts) and 5 Oscillatoriales and 1 Desertifiliales member. All single‐celled species and all Oscillatoriales/Desertifiliales exhibited photophobotaxis, whereas no evidence of photophobotaxis was found for the Nostocales and two other species. A pilus‐free mutant of Synechocystis sp. PCC 6803 did not display this behavior. The photosystem II inhibitor DCMU disrupted photophobotaxis in single‐celled and filamentous cyanobacteria at a concentration of 10 μM; only the filamentous Phormidium lacuna ( P. lacuna ) required 100 μM DCMU for inhibition. This points to PS II as a sensor of photophobotaxis. The widespread occurrence of photophobotaxis aligns with the universality of photosystems. Previous studies on spectral sensitivity and the cyanobacteriochrome PixJ in P. lacuna identified PixJ as a negative regulator of photophobotaxis. In pixJ mutants, light sensitivity was increased compared with the wild‐type. Dual‐wavelength experiments confirmed that yellow light induces PixJ to downregulate photophobotaxis. Our experiments also show that P. lacuna moves faster in darkness than in light and that a temporal change of light intensity from light to dark can induce a change of movement direction. Both findings support the light trap model which is based on random movement and a change of movement direction at the light–dark border. 10.1111/php.70051 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1111/php.70051 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_php_70051 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Photophobotaxis of single‐celled and filamentous cyanobacteria Maria Sinetova Nina Spohrer David Gabrielyan Luke Lehmann Julian Breinich Dmitry Los Tilman Lamparter Photochemistry and Photobiology Photophobotaxis of single‐celled and filamentous cyanobacteria Maria Sinetova Nina Spohrer David Gabrielyan Luke Lehmann Julian Breinich Dmitry Los Tilman Lamparter Photochemistry and Photobiology Abstract Phototaxis refers to an organism's movement toward a light source, while photophobotaxis involves movement into illuminated regions. Although phototaxis in cyanobacteria has been widely studied, photophobotaxis has been investigated in only a few species. In this study, we examined photophobotaxis of 7 single‐celled and 11 filamentous cyanobacterial species, among them 3 Nostocales (filaments with heterocysts) and 5 Oscillatoriales and 1 Desertifiliales member. All single‐celled species and all Oscillatoriales/Desertifiliales exhibited photophobotaxis, whereas no evidence of photophobotaxis was found for the Nostocales and two other species. A pilus‐free mutant of Synechocystis sp. PCC 6803 did not display this behavior. The photosystem II inhibitor DCMU disrupted photophobotaxis in single‐celled and filamentous cyanobacteria at a concentration of 10 μM; only the filamentous Phormidium lacuna ( P. lacuna ) required 100 μM DCMU for inhibition. This points to PS II as a sensor of photophobotaxis. The widespread occurrence of photophobotaxis aligns with the universality of photosystems. Previous studies on spectral sensitivity and the cyanobacteriochrome PixJ in P. lacuna identified PixJ as a negative regulator of photophobotaxis. In pixJ mutants, light sensitivity was increased compared with the wild‐type. Dual‐wavelength experiments confirmed that yellow light induces PixJ to downregulate photophobotaxis. Our experiments also show that P. lacuna moves faster in darkness than in light and that a temporal change of light intensity from light to dark can induce a change of movement direction. Both findings support the light trap model which is based on random movement and a change of movement direction at the light–dark border. 10.1111/php.70051 http://creativecommons.org/licenses/by/4.0/ |
| title | Photophobotaxis of single‐celled and filamentous cyanobacteria |
| topic | Photochemistry and Photobiology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/php.70051 |