Noninvasive evaluation of the skin barrier in reconstructed human epidermis using speckle analysis: Correlation with Raman microspectroscopy
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Wiley
2024
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| author | Léa Habib Léa Abi Nassif Marie Abboud Rime Michael‐Jubeli Ali Tfayli Roger Lteif |
| author_facet | Léa Habib Léa Abi Nassif Marie Abboud Rime Michael‐Jubeli Ali Tfayli Roger Lteif Léa Habib Léa Abi Nassif Marie Abboud Rime Michael‐Jubeli Ali Tfayli Roger Lteif |
| collection | Wiley Open Access |
| contents | Noninvasive evaluation of the skin barrier in reconstructed human epidermis using speckle analysis: Correlation with Raman microspectroscopy Léa Habib Léa Abi Nassif Marie Abboud Rime Michael‐Jubeli Ali Tfayli Roger Lteif Skin Research and Technology Abstract Background Reconstructed epidermis models, obtained from 3D keratinocytes culture, have gained significant prominence as prototypes for safety and efficacy testing in skin research. To effectively evaluate these models, it is essential to perform molecular and functional characterization. The skin's barrier function is one of the essential aspects of the epidermis that needs to be assessed. A noninvasive method is thus required for the evaluation of the skin barrier in these models. With this perspective, the aim of this feasibility study is to apply the speckle technique for the assessment of the skin barrier in the Reconstructed Human Epidermis (RHE). Materials and methods Speckle analysis as well as Raman microspectroscopy were performed on RHE samples at two maturation days, D17 and D20. Results Between D17 and D20, our study showed an increase in various Raman parameters, including stratum corneum percentage, lateral lipid packing, lipid‐to‐protein ratio, and protein secondary structure. Furthermore, the degree of light polarization and the speckle grain size also increased over this period. Conclusion The speckle technique proved to be effective for evaluating the skin barrier in Reconstructed Human Epidermis (RHE) models. Comparison with Raman validates this approach and provides comprehensive molecular and functional characterization of reconstructive skin models. 10.1111/srt.13708 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1111/srt.13708 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_srt_13708 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Noninvasive evaluation of the skin barrier in reconstructed human epidermis using speckle analysis: Correlation with Raman microspectroscopy Léa Habib Léa Abi Nassif Marie Abboud Rime Michael‐Jubeli Ali Tfayli Roger Lteif Skin Research and Technology Noninvasive evaluation of the skin barrier in reconstructed human epidermis using speckle analysis: Correlation with Raman microspectroscopy Léa Habib Léa Abi Nassif Marie Abboud Rime Michael‐Jubeli Ali Tfayli Roger Lteif Skin Research and Technology Abstract Background Reconstructed epidermis models, obtained from 3D keratinocytes culture, have gained significant prominence as prototypes for safety and efficacy testing in skin research. To effectively evaluate these models, it is essential to perform molecular and functional characterization. The skin's barrier function is one of the essential aspects of the epidermis that needs to be assessed. A noninvasive method is thus required for the evaluation of the skin barrier in these models. With this perspective, the aim of this feasibility study is to apply the speckle technique for the assessment of the skin barrier in the Reconstructed Human Epidermis (RHE). Materials and methods Speckle analysis as well as Raman microspectroscopy were performed on RHE samples at two maturation days, D17 and D20. Results Between D17 and D20, our study showed an increase in various Raman parameters, including stratum corneum percentage, lateral lipid packing, lipid‐to‐protein ratio, and protein secondary structure. Furthermore, the degree of light polarization and the speckle grain size also increased over this period. Conclusion The speckle technique proved to be effective for evaluating the skin barrier in Reconstructed Human Epidermis (RHE) models. Comparison with Raman validates this approach and provides comprehensive molecular and functional characterization of reconstructive skin models. 10.1111/srt.13708 http://creativecommons.org/licenses/by/4.0/ |
| title | Noninvasive evaluation of the skin barrier in reconstructed human epidermis using speckle analysis: Correlation with Raman microspectroscopy |
| topic | Skin Research and Technology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/srt.13708 |