Trehalose along with ABA promotes the salt tolerance of Avicennia marina by regulating Na+ transport
Fuente:
Wiley Open Access
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo Open Access |
| Published: |
Wiley
2024
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1867012802743369728 |
|---|---|
| author | Ling‐Yu Song Chao‐Qun Xu Lu‐Dan Zhang Jing Li Li‐Wei Jiang Dong‐Na Ma Ze‐Jun Guo Qian Wang Xiu‐Xiu Wang Hai‐Lei Zheng |
| author_facet | Ling‐Yu Song Chao‐Qun Xu Lu‐Dan Zhang Jing Li Li‐Wei Jiang Dong‐Na Ma Ze‐Jun Guo Qian Wang Xiu‐Xiu Wang Hai‐Lei Zheng Ling‐Yu Song Chao‐Qun Xu Lu‐Dan Zhang Jing Li Li‐Wei Jiang Dong‐Na Ma Ze‐Jun Guo Qian Wang Xiu‐Xiu Wang Hai‐Lei Zheng |
| collection | Wiley Open Access |
| contents | Trehalose along with ABA promotes the salt tolerance of Avicennia marina by regulating Na+ transport Ling‐Yu Song Chao‐Qun Xu Lu‐Dan Zhang Jing Li Li‐Wei Jiang Dong‐Na Ma Ze‐Jun Guo Qian Wang Xiu‐Xiu Wang Hai‐Lei Zheng The Plant Journal SUMMARYMangroves grow in tropical/subtropical intertidal habitats with extremely high salt tolerance. Trehalose and trehalose‐6‐phosphate (T6P) have an alleviating function against abiotic stress. However, the roles of trehalose in the salt tolerance of salt‐secreting mangrove Avicennia marina is not documented. Here, we found that trehalose was significantly accumulated in A. marina under salt treatment. Furthermore, exogenous trehalose can enhance salt tolerance by promoting the Na+ efflux from leaf salt gland and root to reduce the Na+ content in root and leaf. Subsequently, eighteen trehalose‐6‐phosphate synthase (AmTPS) and 11 trehalose‐6‐phosphate phosphatase (AmTPP) genes were identified from A. marina genome. Abscisic acid (ABA) responsive elements were predicted in AmTPS and AmTPP promoters by cis‐acting elements analysis. We further identified AmTPS9A, as an important positive regulator, that increased the salt tolerance of AmTPS9A‐overexpressing Arabidopsis thaliana by altering the expressions of ion transport genes and mediating Na+ efflux from the roots of transgenic A. thaliana under NaCl treatments. In addition, we also found that ABA could promote the accumulation of trehalose, and the application of exogenous trehalose significantly promoted the biosynthesis of ABA in both roots and leaves of A. marina. Ultimately, we confirmed that AmABF2 directly binds to the AmTPS9A promoter in vitro and in vivo. Taken together, we speculated that there was a positive feedback loop between trehalose and ABA in regulating the salt tolerance of A. marina. These findings provide new understanding to the salt tolerance of A. marina in adapting to high saline environment at trehalose and ABA aspects. 10.1111/tpj.16921 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1111/tpj.16921 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_tpj_16921 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Trehalose along with ABA promotes the salt tolerance of Avicennia marina by regulating Na+ transport Ling‐Yu Song Chao‐Qun Xu Lu‐Dan Zhang Jing Li Li‐Wei Jiang Dong‐Na Ma Ze‐Jun Guo Qian Wang Xiu‐Xiu Wang Hai‐Lei Zheng The Plant Journal Trehalose along with ABA promotes the salt tolerance of Avicennia marina by regulating Na+ transport Ling‐Yu Song Chao‐Qun Xu Lu‐Dan Zhang Jing Li Li‐Wei Jiang Dong‐Na Ma Ze‐Jun Guo Qian Wang Xiu‐Xiu Wang Hai‐Lei Zheng The Plant Journal SUMMARYMangroves grow in tropical/subtropical intertidal habitats with extremely high salt tolerance. Trehalose and trehalose‐6‐phosphate (T6P) have an alleviating function against abiotic stress. However, the roles of trehalose in the salt tolerance of salt‐secreting mangrove Avicennia marina is not documented. Here, we found that trehalose was significantly accumulated in A. marina under salt treatment. Furthermore, exogenous trehalose can enhance salt tolerance by promoting the Na+ efflux from leaf salt gland and root to reduce the Na+ content in root and leaf. Subsequently, eighteen trehalose‐6‐phosphate synthase (AmTPS) and 11 trehalose‐6‐phosphate phosphatase (AmTPP) genes were identified from A. marina genome. Abscisic acid (ABA) responsive elements were predicted in AmTPS and AmTPP promoters by cis‐acting elements analysis. We further identified AmTPS9A, as an important positive regulator, that increased the salt tolerance of AmTPS9A‐overexpressing Arabidopsis thaliana by altering the expressions of ion transport genes and mediating Na+ efflux from the roots of transgenic A. thaliana under NaCl treatments. In addition, we also found that ABA could promote the accumulation of trehalose, and the application of exogenous trehalose significantly promoted the biosynthesis of ABA in both roots and leaves of A. marina. Ultimately, we confirmed that AmABF2 directly binds to the AmTPS9A promoter in vitro and in vivo. Taken together, we speculated that there was a positive feedback loop between trehalose and ABA in regulating the salt tolerance of A. marina. These findings provide new understanding to the salt tolerance of A. marina in adapting to high saline environment at trehalose and ABA aspects. 10.1111/tpj.16921 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Trehalose along with ABA promotes the salt tolerance of Avicennia marina by regulating Na+ transport |
| topic | The Plant Journal |
| url | https://onlinelibrary.wiley.com/doi/10.1111/tpj.16921 |