Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Artículo Open Access |
| Published: |
Wiley
2024
|
| Subjects: | |
| Online Access: | https://physoc.onlinelibrary.wiley.com/doi/10.14814/phy2.15921 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1867008499874004992 |
|---|---|
| author | Stephanie A. Brocke Adam M. Speen Syed Masood Cameron P. Worden Ilona Jaspers |
| author_facet | Stephanie A. Brocke Adam M. Speen Syed Masood Cameron P. Worden Ilona Jaspers Stephanie A. Brocke Adam M. Speen Syed Masood Cameron P. Worden Ilona Jaspers |
| collection | Wiley Open Access |
| contents | Comparison of permeable cell culture inserts for use in culture of a human in vitro air–liquid interface model system Stephanie A. Brocke Adam M. Speen Syed Masood Cameron P. Worden Ilona Jaspers Physiological Reports AbstractIn this study, we compared 12 mm cell culture inserts with permeable polyester membranes (0.4 μm pores) from two different manufacturers: CELLTREAT® and Corning®. Physical dimensions and masses of the inserts were found to be very similar between the two brands, with CELLTREAT® inserts having a slightly smaller diameter and growth area (11.91 mm; 1.11 cm2) compared to Corning® Transwells® (12 mm; 1.13 cm2). We compared cell differentiation outcomes of human nasal epithelial cells (HNECs) at air–liquid interface grown on inserts from the two different manufacturers, including trans‐epithelial electrical resistance, ciliary beat frequency, ciliated area, and gene expression. HNECs from three male donors were used for all endpoints. No statistically significant differences were observed between paired cultures grown on different brands of insert. In conclusion, these inserts are comparable for use with airway epithelial cell model systems and likely do not impact cellular differentiation or cell culture quality. 10.14814/phy2.15921 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.14814/phy2.15921 |
| format | Artículo Open Access |
| id | wiley_oa_10_14814_phy2_15921 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Comparison of permeable cell culture inserts for use in culture of a human in vitro air–liquid interface model system Stephanie A. Brocke Adam M. Speen Syed Masood Cameron P. Worden Ilona Jaspers Physiological Reports Comparison of permeable cell culture inserts for use in culture of a human in vitro air–liquid interface model system Stephanie A. Brocke Adam M. Speen Syed Masood Cameron P. Worden Ilona Jaspers Physiological Reports AbstractIn this study, we compared 12 mm cell culture inserts with permeable polyester membranes (0.4 μm pores) from two different manufacturers: CELLTREAT® and Corning®. Physical dimensions and masses of the inserts were found to be very similar between the two brands, with CELLTREAT® inserts having a slightly smaller diameter and growth area (11.91 mm; 1.11 cm2) compared to Corning® Transwells® (12 mm; 1.13 cm2). We compared cell differentiation outcomes of human nasal epithelial cells (HNECs) at air–liquid interface grown on inserts from the two different manufacturers, including trans‐epithelial electrical resistance, ciliary beat frequency, ciliated area, and gene expression. HNECs from three male donors were used for all endpoints. No statistically significant differences were observed between paired cultures grown on different brands of insert. In conclusion, these inserts are comparable for use with airway epithelial cell model systems and likely do not impact cellular differentiation or cell culture quality. 10.14814/phy2.15921 http://creativecommons.org/licenses/by/4.0/ |
| title | Comparison of permeable cell culture inserts for use in culture of a human in vitro air–liquid interface model system |
| topic | Physiological Reports |
| url | https://physoc.onlinelibrary.wiley.com/doi/10.14814/phy2.15921 |