Discovery of the Alternaria mycotoxins alterperylenol and altertoxin I as novel immunosuppressive and antiestrogenic compounds in vitro
Fuente:
Zenodo
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Recurso digital |
| Lenguaje: | inglés |
| Publicado: |
Zenodo
2024
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1866901252722393088 |
|---|---|
| author | Crudo, Francesco Partsch, Vanessa Braga, Dennis Blažević, Ruzica Rollinger, Judith Varga, Elisabeth Marko, Doris |
| author_facet | Crudo, Francesco Partsch, Vanessa Braga, Dennis Blažević, Ruzica Rollinger, Judith Varga, Elisabeth Marko, Doris |
| contents | <p>Alternaria mycotoxins may pose signifcant challenges to food safety and public health due to the wide spectrum of reported<br>adverse efects. Despite this, critical information on the immunomodulatory and antiestrogenic properties of most of these<br>contaminants is still lacking. The present study aimed to identify the mycotoxins responsible for the immunosuppressive<br>and antiestrogenic efects of a complex extract of Alternaria mycotoxins (CE) obtained by growing an Alternaria alternata<br>strain on rice. Through a toxicity-guided fractionation procedure involving the production of CE-fractions by supercritical<br>fuid chromatography and mycotoxin quantifcation by LC–MS/MS, the mycotoxins alternariol (AOH), tenuazonic acid<br>(TeA), altertoxin I (ATX-I), and alterperylenol (ALTP) were identifed as potential toxicologically relevant constituents<br>contributing to the in vitro efects exerted by the extract. The assessment of the immunomodulatory efects, performed by<br>applying the NF-κB reporter gene assay in THP1-Lucia™ monocytes, revealed the scarce contribution of AOH to the efects<br>exerted by the CE. TeA showed no efect on the NF-κB pathway up to 250 µM, whereas ATX-I and ALTP suppressed the<br>LPS-mediated pathway activation at concentrations≥1 µM. The evaluation of antiestrogenic efects, performed in Ishikawa<br>cells by applying the alkaline phosphatase assay, revealed the ability of ALTP (≥0.4 µM) and ATX-I (≥2 µM) to suppress<br>the estrogen-dependent expression of enzyme activity. Given the risk of detrimental impacts stemming from alterations<br>in endocrine and systemic immune responses by the investigated mycotoxins, further studies are needed to elucidate their<br>underlying mechanisms of action and comprehensively evaluate the health risks posed by these toxins.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_1007_s00204-024-03877-1 |
| institution | Zenodo |
| language | eng |
| publishDate | 2024 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Discovery of the Alternaria mycotoxins alterperylenol and altertoxin I as novel immunosuppressive and antiestrogenic compounds in vitro Crudo, Francesco Partsch, Vanessa Braga, Dennis Blažević, Ruzica Rollinger, Judith Varga, Elisabeth Marko, Doris In vitro toxicity chemical mixtures food contaminants Alternaria mycotoxins <p>Alternaria mycotoxins may pose signifcant challenges to food safety and public health due to the wide spectrum of reported<br>adverse efects. Despite this, critical information on the immunomodulatory and antiestrogenic properties of most of these<br>contaminants is still lacking. The present study aimed to identify the mycotoxins responsible for the immunosuppressive<br>and antiestrogenic efects of a complex extract of Alternaria mycotoxins (CE) obtained by growing an Alternaria alternata<br>strain on rice. Through a toxicity-guided fractionation procedure involving the production of CE-fractions by supercritical<br>fuid chromatography and mycotoxin quantifcation by LC–MS/MS, the mycotoxins alternariol (AOH), tenuazonic acid<br>(TeA), altertoxin I (ATX-I), and alterperylenol (ALTP) were identifed as potential toxicologically relevant constituents<br>contributing to the in vitro efects exerted by the extract. The assessment of the immunomodulatory efects, performed by<br>applying the NF-κB reporter gene assay in THP1-Lucia™ monocytes, revealed the scarce contribution of AOH to the efects<br>exerted by the CE. TeA showed no efect on the NF-κB pathway up to 250 µM, whereas ATX-I and ALTP suppressed the<br>LPS-mediated pathway activation at concentrations≥1 µM. The evaluation of antiestrogenic efects, performed in Ishikawa<br>cells by applying the alkaline phosphatase assay, revealed the ability of ALTP (≥0.4 µM) and ATX-I (≥2 µM) to suppress<br>the estrogen-dependent expression of enzyme activity. Given the risk of detrimental impacts stemming from alterations<br>in endocrine and systemic immune responses by the investigated mycotoxins, further studies are needed to elucidate their<br>underlying mechanisms of action and comprehensively evaluate the health risks posed by these toxins.</p> |
| title | Discovery of the Alternaria mycotoxins alterperylenol and altertoxin I as novel immunosuppressive and antiestrogenic compounds in vitro |
| topic | In vitro toxicity chemical mixtures food contaminants Alternaria mycotoxins |
| url | https://doi.org/10.1007/s00204-024-03877-1 |