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2025
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| Online Access: | https://doi.org/10.1007/s00705-025-06322-5 |
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| author | Middelboe, Mathias Dogsa, Iztok Lazar, Jaka Jakin Šimunović, Katarina Dragoš, Anna Mulec, Ines Mandić |
| author_facet | Middelboe, Mathias Dogsa, Iztok Lazar, Jaka Jakin Šimunović, Katarina Dragoš, Anna Mulec, Ines Mandić |
| contents | <p>Phage-host interactions that occur in host-associated microbiomes are influenced by a plethora of environmental factors.<br>Mucins are glycoproteins that represent the main component of mucus, which is found in the animal digestive tract and<br>on the surface of certain organs, serving as the first line of defense against toxins and pathogens. Previous studies have<br>shown that lytic phages have an important influence on the microbial composition in mucosal areas. Our study expands this<br>knowledge to interactions between previously untested lytic phages targeting probiotic and pathogenic bacteria, as well as<br>temperate phages targeting probiotic bacteria. These interactions could be important in shaping microbial communities and<br>affecting the well-being of their host. This study demonstrates that mucins enhance the adherence of Vibrio anguillarum<br>lytic phages and Bacillus subtilis lytic and temperate phages, as well as B. subtilis and V. anguillarum cells, to solid surfaces.<br>Our results also show that mucins positively affect the attachment of B. subtilis cells even in the presence of phages. This<br>positive effect was not observed in the case of V. anguillarum. This suggests that mucin may shield certain bacteria from<br>phage infections. We also found that mucin influenced the metabolic activity of the two tested bacterial species differently,<br>with strong positive effects on V. anguillarum but not on B. subtilis. This work supports previous findings that phages adhere<br>efficiently to mucus and extends these studies to include other beneficial and pathogenic bacterial species. It also reveals<br>that mucins have different effects on phage-host interactions in different phage-host systems, which may have implications<br>for phage therapies or probiotic treatment strategies.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_1007_s00705-025-06322-5 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Distinct effects of mucin on phage‑host interactions in model systems of beneficial and pathogenic bacteria Middelboe, Mathias Dogsa, Iztok Lazar, Jaka Jakin Šimunović, Katarina Dragoš, Anna Mulec, Ines Mandić <p>Phage-host interactions that occur in host-associated microbiomes are influenced by a plethora of environmental factors.<br>Mucins are glycoproteins that represent the main component of mucus, which is found in the animal digestive tract and<br>on the surface of certain organs, serving as the first line of defense against toxins and pathogens. Previous studies have<br>shown that lytic phages have an important influence on the microbial composition in mucosal areas. Our study expands this<br>knowledge to interactions between previously untested lytic phages targeting probiotic and pathogenic bacteria, as well as<br>temperate phages targeting probiotic bacteria. These interactions could be important in shaping microbial communities and<br>affecting the well-being of their host. This study demonstrates that mucins enhance the adherence of Vibrio anguillarum<br>lytic phages and Bacillus subtilis lytic and temperate phages, as well as B. subtilis and V. anguillarum cells, to solid surfaces.<br>Our results also show that mucins positively affect the attachment of B. subtilis cells even in the presence of phages. This<br>positive effect was not observed in the case of V. anguillarum. This suggests that mucin may shield certain bacteria from<br>phage infections. We also found that mucin influenced the metabolic activity of the two tested bacterial species differently,<br>with strong positive effects on V. anguillarum but not on B. subtilis. This work supports previous findings that phages adhere<br>efficiently to mucus and extends these studies to include other beneficial and pathogenic bacterial species. It also reveals<br>that mucins have different effects on phage-host interactions in different phage-host systems, which may have implications<br>for phage therapies or probiotic treatment strategies.</p> |
| title | Distinct effects of mucin on phage‑host interactions in model systems of beneficial and pathogenic bacteria |
| url | https://doi.org/10.1007/s00705-025-06322-5 |