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| Format: | Recurso digital |
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Zenodo
2025
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| Online Access: | https://doi.org/10.3390/cells15010018 |
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| _version_ | 1866901935106293760 |
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| author | Mousavi, Seyed Omid Reza Reshi, Qurat Ul Ain Godakumara, Kasun Muhandiram, Subhashini Midekessa, Getnet Andronowska, Aneta Kopanchuk, Sergei Lavogina, Darja Lavogina, Darja Rinken, Ago Kodithuwakku, Suranga Fazeli, Alireza |
| author_facet | Mousavi, Seyed Omid Reza Reshi, Qurat Ul Ain Godakumara, Kasun Muhandiram, Subhashini Midekessa, Getnet Andronowska, Aneta Kopanchuk, Sergei Lavogina, Darja Lavogina, Darja Rinken, Ago Kodithuwakku, Suranga Fazeli, Alireza |
| contents | <p>Extracellular vesicles (EVs) are promising therapeutic agents due to their role in intercellular communication. This study examined the protective effects of milk-derived EVs (mEVs) on bovine oviductal epithelial cells (BOECs) under cobalt chloride (CoCl<sub>2</sub>)-induced oxidative stress (OS), comparing EVs stored at −80 °C or lyophilized. mEVs and algae-derived EVs (aEVs; negative control) were isolated via tangential flow filtration and applied at 10<sup>7</sup>, 10<sup>9</sup>, and 10<sup>11</sup> particles/mL in three treatment strategies: pre-treatment, co-incubation, and post-treatment. mEVs specifically enhanced cell viability in all protocols except for post-treatment, where only 10<sup>7</sup> particles/mL was effective; meanwhile, storage method did not affect EV activity. Enzyme digestion suggested that internal EV cargos are potentially the dominant contributors to the protective response compared to surface-associated molecules. mEVs reduced the expression of the OS markers <span>DDIT4</span> and <span>HIF1A</span> while promoting cell migration more effectively than aEVs. Pathway enrichment analysis of previously reported mEV miRNAs indicated regulation of cytokine production and glucocorticoid responses, potentially contributing to OS defense. mEV protein cargo analysis showed pathways primarily linked to peptidase and vesicle-related functions, suggesting that protein cargo may also contribute to the observed protective effects. Overall, mEVs protect BOECs against CoCl<sub>2</sub>-induced OS and maintain bioactivity after lyophilization.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_3390_cells15010018 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Milk-Derived Extracellular Vesicles Protect Bovine Oviduct Epithelial Cells from Oxidative Stress Mousavi, Seyed Omid Reza Reshi, Qurat Ul Ain Godakumara, Kasun Muhandiram, Subhashini Midekessa, Getnet Andronowska, Aneta Kopanchuk, Sergei Lavogina, Darja Lavogina, Darja Rinken, Ago Kodithuwakku, Suranga Fazeli, Alireza <p>Extracellular vesicles (EVs) are promising therapeutic agents due to their role in intercellular communication. This study examined the protective effects of milk-derived EVs (mEVs) on bovine oviductal epithelial cells (BOECs) under cobalt chloride (CoCl<sub>2</sub>)-induced oxidative stress (OS), comparing EVs stored at −80 °C or lyophilized. mEVs and algae-derived EVs (aEVs; negative control) were isolated via tangential flow filtration and applied at 10<sup>7</sup>, 10<sup>9</sup>, and 10<sup>11</sup> particles/mL in three treatment strategies: pre-treatment, co-incubation, and post-treatment. mEVs specifically enhanced cell viability in all protocols except for post-treatment, where only 10<sup>7</sup> particles/mL was effective; meanwhile, storage method did not affect EV activity. Enzyme digestion suggested that internal EV cargos are potentially the dominant contributors to the protective response compared to surface-associated molecules. mEVs reduced the expression of the OS markers <span>DDIT4</span> and <span>HIF1A</span> while promoting cell migration more effectively than aEVs. Pathway enrichment analysis of previously reported mEV miRNAs indicated regulation of cytokine production and glucocorticoid responses, potentially contributing to OS defense. mEV protein cargo analysis showed pathways primarily linked to peptidase and vesicle-related functions, suggesting that protein cargo may also contribute to the observed protective effects. Overall, mEVs protect BOECs against CoCl<sub>2</sub>-induced OS and maintain bioactivity after lyophilization.</p> |
| title | Milk-Derived Extracellular Vesicles Protect Bovine Oviduct Epithelial Cells from Oxidative Stress |
| url | https://doi.org/10.3390/cells15010018 |