Expression profiling by high throughput sequencing
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| Formato: | Recurso digital |
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2024
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| _version_ | 1866902280331067392 |
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| author | Xiaofeng, Zhang |
| author_facet | Xiaofeng, Zhang |
| contents | <h3><span>Single-cell RNA-seq libraries of HCC tumor tissues from PDX were generated using the 10x Genomics Chromium Single Cell 3' Reagent Kit (v3) following the manufacturer's protocol ("ChromiumNextGEMSingleCell3_v3.1_Rev_D"). The libraries were sequenced on an Illumina NovaSeq 6000 platform (Illumina) with paired-end 150bp reads. <span>Raw sequencing reads were preprocessed using Cell Ranger (version 6.1.2) software provided by 10x Genomics with reference data “refdata-gex-GRCh38-and-mm10-2020-A”. Cells and genes identified as mouse-oriented were filtered. </span></span></h3> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_11044640 |
| institution | Zenodo |
| language | |
| publishDate | 2024 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Expression profiling by high throughput sequencing Xiaofeng, Zhang <h3><span>Single-cell RNA-seq libraries of HCC tumor tissues from PDX were generated using the 10x Genomics Chromium Single Cell 3' Reagent Kit (v3) following the manufacturer's protocol ("ChromiumNextGEMSingleCell3_v3.1_Rev_D"). The libraries were sequenced on an Illumina NovaSeq 6000 platform (Illumina) with paired-end 150bp reads. <span>Raw sequencing reads were preprocessed using Cell Ranger (version 6.1.2) software provided by 10x Genomics with reference data “refdata-gex-GRCh38-and-mm10-2020-A”. Cells and genes identified as mouse-oriented were filtered. </span></span></h3> |
| title | Expression profiling by high throughput sequencing |
| url | https://doi.org/10.5281/zenodo.11044640 |