Nanopores with an Engineered Selective Entropic Gate DetectProteins at Nanomolar Concentration in Complex Biological Sample
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| Format: | Recurso digital |
| Langue: | anglais |
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2025
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| _version_ | 1866901056276922368 |
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| author | Straathof, Sabine Di Muccio, Giovanni |
| author_facet | Straathof, Sabine Di Muccio, Giovanni |
| contents | <p>This dataset belongs to the article: "Nanopores with an Engineered Selective Entropic Gate DetectProteins at Nanomolar Concentration in Complex Biological Sample" published in JACS (DOI: 10.1021/jacs.4c17147) and contains the raw electrophysiology data of protein capture by engineered YaxAB, analysis MATLAB scripts and analysis Excel files. </p> <p>For YaxAB_JACS_2025.zip:</p> <p>- Each electrophysiology file contains measurement info as follows:<br>[Date of measurement]_[Buffer conditions]_[Pore type]_[added analyte(s)]_[operator initials]</p> <p>- Each electrophys trace is accompanied by Excel file with Clampfit analysis in Sheet1 (named [voltage], with the columns corresponding to: Trace; Search; Level; State; Event Start Time (ms); Event End Time (ms); Amplitude (pA); Amp S.D. (pA); Dwell Time (ms); Inst. Freq. (Hz); Interevent Interval (ms); [empy]; open pore current (pA)</p> <p>- This Excel sheet was analyzed with inhouse MATLAB script; and event data (Iex, dwell time, Amp S.D.) is placed in second sheet of Excel file.</p> <p>- A summary of all the event data (mean +/- S.D.) for all replicates is in a separate Excel file in the corresponding folder. </p> <p> </p> <p>This dataset contains one replicate per experiment. Raw data for all replicates can be shared upon reasonable request. </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_13132666 |
| institution | Zenodo |
| language | eng |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Nanopores with an Engineered Selective Entropic Gate DetectProteins at Nanomolar Concentration in Complex Biological Sample Straathof, Sabine Di Muccio, Giovanni electrophysiology nanopores nuclear pore comples entropic gate biosensing <p>This dataset belongs to the article: "Nanopores with an Engineered Selective Entropic Gate DetectProteins at Nanomolar Concentration in Complex Biological Sample" published in JACS (DOI: 10.1021/jacs.4c17147) and contains the raw electrophysiology data of protein capture by engineered YaxAB, analysis MATLAB scripts and analysis Excel files. </p> <p>For YaxAB_JACS_2025.zip:</p> <p>- Each electrophysiology file contains measurement info as follows:<br>[Date of measurement]_[Buffer conditions]_[Pore type]_[added analyte(s)]_[operator initials]</p> <p>- Each electrophys trace is accompanied by Excel file with Clampfit analysis in Sheet1 (named [voltage], with the columns corresponding to: Trace; Search; Level; State; Event Start Time (ms); Event End Time (ms); Amplitude (pA); Amp S.D. (pA); Dwell Time (ms); Inst. Freq. (Hz); Interevent Interval (ms); [empy]; open pore current (pA)</p> <p>- This Excel sheet was analyzed with inhouse MATLAB script; and event data (Iex, dwell time, Amp S.D.) is placed in second sheet of Excel file.</p> <p>- A summary of all the event data (mean +/- S.D.) for all replicates is in a separate Excel file in the corresponding folder. </p> <p> </p> <p>This dataset contains one replicate per experiment. Raw data for all replicates can be shared upon reasonable request. </p> |
| title | Nanopores with an Engineered Selective Entropic Gate DetectProteins at Nanomolar Concentration in Complex Biological Sample |
| topic | electrophysiology nanopores nuclear pore comples entropic gate biosensing |
| url | https://doi.org/10.5281/zenodo.13132666 |