Polymerase Chain Reaction Assay and Conventional Isolation of Salmonella spp. from Philippine Bats
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Zenodo
2011
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| _version_ | 1866901446822199296 |
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| author | Reyes, Alisha Wehdnesday Bernardo Rovira, Hope Guerrero Masangkay, Joseph Saavedra Juan, Teresita Yoshikawa, Yasuhiro Baticados, Waren Navarra Ramirez, Teresita Juan |
| author_facet | Reyes, Alisha Wehdnesday Bernardo Rovira, Hope Guerrero Masangkay, Joseph Saavedra Juan, Teresita Yoshikawa, Yasuhiro Baticados, Waren Navarra Ramirez, Teresita Juan |
| contents | (Uploaded by Plazi for the Bat Literature Project) Background: Salmonellae are important food and waterborne pathogens and the leading causes of the most widespread acute gastrointestinal illnesses around the globe. The organism has been detected in a wide range of host species such as mites, insects, crustaceans, mussels, fish, amphibians, reptiles, birds and mammals including wildlife animals. Salmonellae have been isolated in many species of bats in other countries. In the Philippines, there are 70 species of Philippine bats reported of which nine are considered as endemic. Although human salmonellosis (typhoid, paratyphoid and other Salmonella-associated infections) was the primary cause of illnesses and death from the 60 reported foodborne outbreaks (1995 to 2004), no case was ever reported involving Philippine bats. Since transmission of Salmonella from wildlife to humans is possible, as advocated by previous reports, the present study endeavored to isolate and molecularly detect Salmonella spp. from Philippine bats captured from Aklan, Laguna and Quezon City using conventional isolation method and polymerase chain reaction assay respectively. Materials, Methods & Results: A total of 96 apparently healthy bats were used in the study. Bats were captured using nylon mobile mist nets of 3 m long and 1.5 m high with 35 mm mesh size. Eleven species of bats were collected and identified following the reported key to the identification of Philippine bats. Majority of the collected species were insectivores under family Vespertilionidae while the largest population of the Philippine bats were frugivores belonging to family Pteropodidae. Necropsy was performed and intestines were collected and subjected to conventional culture method and PCR detection for Salmonella spp. Two samples (2.08%) were molecularly detected as positive for Salmonella spp. bacterial pathogen. The positive samples were obtained from the intestines of the adult female insectivorous bat species, Miniopterus australis and M. schreibersi, originating from Pangihan cave of Barangay Pablacion, Malay in Aklan. No Salmonella spp. was isolated using the conventional method. |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_13433374 |
| institution | Zenodo |
| language | |
| publishDate | 2011 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Polymerase Chain Reaction Assay and Conventional Isolation of Salmonella spp. from Philippine Bats Reyes, Alisha Wehdnesday Bernardo Rovira, Hope Guerrero Masangkay, Joseph Saavedra Juan, Teresita Yoshikawa, Yasuhiro Baticados, Waren Navarra Ramirez, Teresita Juan Biodiversity Mammalia Chiroptera Chordata Animalia bats bat (Uploaded by Plazi for the Bat Literature Project) Background: Salmonellae are important food and waterborne pathogens and the leading causes of the most widespread acute gastrointestinal illnesses around the globe. The organism has been detected in a wide range of host species such as mites, insects, crustaceans, mussels, fish, amphibians, reptiles, birds and mammals including wildlife animals. Salmonellae have been isolated in many species of bats in other countries. In the Philippines, there are 70 species of Philippine bats reported of which nine are considered as endemic. Although human salmonellosis (typhoid, paratyphoid and other Salmonella-associated infections) was the primary cause of illnesses and death from the 60 reported foodborne outbreaks (1995 to 2004), no case was ever reported involving Philippine bats. Since transmission of Salmonella from wildlife to humans is possible, as advocated by previous reports, the present study endeavored to isolate and molecularly detect Salmonella spp. from Philippine bats captured from Aklan, Laguna and Quezon City using conventional isolation method and polymerase chain reaction assay respectively. Materials, Methods & Results: A total of 96 apparently healthy bats were used in the study. Bats were captured using nylon mobile mist nets of 3 m long and 1.5 m high with 35 mm mesh size. Eleven species of bats were collected and identified following the reported key to the identification of Philippine bats. Majority of the collected species were insectivores under family Vespertilionidae while the largest population of the Philippine bats were frugivores belonging to family Pteropodidae. Necropsy was performed and intestines were collected and subjected to conventional culture method and PCR detection for Salmonella spp. Two samples (2.08%) were molecularly detected as positive for Salmonella spp. bacterial pathogen. The positive samples were obtained from the intestines of the adult female insectivorous bat species, Miniopterus australis and M. schreibersi, originating from Pangihan cave of Barangay Pablacion, Malay in Aklan. No Salmonella spp. was isolated using the conventional method. |
| title | Polymerase Chain Reaction Assay and Conventional Isolation of Salmonella spp. from Philippine Bats |
| topic | Biodiversity Mammalia Chiroptera Chordata Animalia bats bat |
| url | https://doi.org/10.5281/zenodo.13433374 |