Bats as a reservoir of resistant Escherichia coli: A methodical view. Can we fully estimate the scale of resistance in the reservoirs of free-living animals?
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2020
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| author | Nowakiewicz, Aneta Zięba, Przemysław Gnat, Sebastian Trościańczyk, Aleksandra Osińska, Marcelina Łagowski, Dominik Kosior-Korzecka, Urszula Puzio, Iwona |
| author_facet | Nowakiewicz, Aneta Zięba, Przemysław Gnat, Sebastian Trościańczyk, Aleksandra Osińska, Marcelina Łagowski, Dominik Kosior-Korzecka, Urszula Puzio, Iwona |
| contents | (Uploaded by Plazi for the Bat Literature Project) Bats are a poorly understood reservoir of pathogenic and multi-drug resistant microorganisms; therefore, the aim of the study was to analyze the presence of drug resistance among E. coli isolated from the species of bats occurring naturally in Poland. The strategy of isolation and identification of resistant strains from pooled and single-animal samples was based on selective media with cefotaxime, chloramphenicol, kanamycin and tetracycline, the use of the ADSRRS-fingerprinting method for genomic differentiation of isolates, and the classical methods of evaluation of phenotypic and genotypic resistance. Of the 78 isolated isolates confirmed as E. coli, there were 38 genetically distinct strains resistant at least to one antimicrobial. 71% of these strains met the multi-drug resistance criterion. Moreover, two different multidrug resistant strains were isolated from three single samples. The highest resistance was observed in the case of ampicillin (66%), kanamycin (84%), sulfamethoxazole/trimetoprim (61%/55% respectively), and streptomycin (50%), which in most cases was confirmed by the presence of an adequate gene. Two isolates from single hosts produced extended-spectrum beta-lactamases (bla CTX-M-3, bla CTX-M-15, bla TEM-1). With the exception of tetracycline resistance, which was dominant among isolates from single animals, no significant differences in the resistance of the strains from both groups of samples were observed. |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_13440557 |
| institution | Zenodo |
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| publishDate | 2020 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Bats as a reservoir of resistant Escherichia coli: A methodical view. Can we fully estimate the scale of resistance in the reservoirs of free-living animals? Nowakiewicz, Aneta Zięba, Przemysław Gnat, Sebastian Trościańczyk, Aleksandra Osińska, Marcelina Łagowski, Dominik Kosior-Korzecka, Urszula Puzio, Iwona Biodiversity Mammalia Chiroptera Chordata Animalia bats bat (Uploaded by Plazi for the Bat Literature Project) Bats are a poorly understood reservoir of pathogenic and multi-drug resistant microorganisms; therefore, the aim of the study was to analyze the presence of drug resistance among E. coli isolated from the species of bats occurring naturally in Poland. The strategy of isolation and identification of resistant strains from pooled and single-animal samples was based on selective media with cefotaxime, chloramphenicol, kanamycin and tetracycline, the use of the ADSRRS-fingerprinting method for genomic differentiation of isolates, and the classical methods of evaluation of phenotypic and genotypic resistance. Of the 78 isolated isolates confirmed as E. coli, there were 38 genetically distinct strains resistant at least to one antimicrobial. 71% of these strains met the multi-drug resistance criterion. Moreover, two different multidrug resistant strains were isolated from three single samples. The highest resistance was observed in the case of ampicillin (66%), kanamycin (84%), sulfamethoxazole/trimetoprim (61%/55% respectively), and streptomycin (50%), which in most cases was confirmed by the presence of an adequate gene. Two isolates from single hosts produced extended-spectrum beta-lactamases (bla CTX-M-3, bla CTX-M-15, bla TEM-1). With the exception of tetracycline resistance, which was dominant among isolates from single animals, no significant differences in the resistance of the strains from both groups of samples were observed. |
| title | Bats as a reservoir of resistant Escherichia coli: A methodical view. Can we fully estimate the scale of resistance in the reservoirs of free-living animals? |
| topic | Biodiversity Mammalia Chiroptera Chordata Animalia bats bat |
| url | https://doi.org/10.5281/zenodo.13440557 |