Antimicrobial resistance in Escherichia coli isolates from frugivorous (Eidolon helvum) and insectivorous (Nycteris hispida) bats in Southeast Nigeria, with detection of CTX-M-15 producing isolates

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Main Authors: Obodoechi, Lynda O., Carvalho, Isabel, Chenouf, Nadia Safia, Martínez-Álvarez, Sandra, Sadi, Madjid, Nwanta, John A., Chah, Kennedy F., Torres, Carmen
Format: Recurso digital
Published: Zenodo 2021
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author Obodoechi, Lynda O.
Carvalho, Isabel
Chenouf, Nadia Safia
Martínez-Álvarez, Sandra
Sadi, Madjid
Nwanta, John A.
Chah, Kennedy F.
Torres, Carmen
author_facet Obodoechi, Lynda O.
Carvalho, Isabel
Chenouf, Nadia Safia
Martínez-Álvarez, Sandra
Sadi, Madjid
Nwanta, John A.
Chah, Kennedy F.
Torres, Carmen
contents (Uploaded by Plazi for the Bat Literature Project) Thirty-five Escherichia coli isolates obtained from the liver, spleen and intestines of 180 frugivorous and insec­ tivorous bats were investigated for antimicrobial resistance phenotypes/genotypes, prevalence of ExtendedSpectrum beta-lactamase (ESBL) production, virulence gene detection and molecular typing. Eight (22.9 %) of the isolates were multidrug resistant (MDR). Two isolates were cefotaxime-resistant, ESBL-producers and harbored the blaCTX-M-15 gene; they belonged to ST10184-D and ST2178-B1 lineages. tet(A) gene was detected in all tetracycline-resistant isolates while int1 (n = 8) and blaTEM (n = 7) genes were also found. Thirty-three of the E. coli isolates were assigned to seven phylogenetic groups, with B1 (45.7 %) being predominant. Three isolates were enteropathogenic E. coli (EPEC) pathovars, containing the eae gene (with the variants gamma and iota), and lacking stx1/stx2 genes. Bats in Nigeria are possible reservoirs of potentially pathogenic MDR E. coli isolates which may be important in the ecology of antimicrobial resistance at the human-livestock-wildlife-environment interfaces. The study reinforces the importance of including wildlife in national antimicrobial resistance moni­ toring programmes.
format Recurso digital
id zenodo_https___doi_org_10_5281_zenodo_13466534
institution Zenodo
language
publishDate 2021
publisher Zenodo
record_format zenodo
spellingShingle Antimicrobial resistance in Escherichia coli isolates from frugivorous (Eidolon helvum) and insectivorous (Nycteris hispida) bats in Southeast Nigeria, with detection of CTX-M-15 producing isolates
Obodoechi, Lynda O.
Carvalho, Isabel
Chenouf, Nadia Safia
Martínez-Álvarez, Sandra
Sadi, Madjid
Nwanta, John A.
Chah, Kennedy F.
Torres, Carmen
Biodiversity
Mammalia
Chiroptera
Chordata
Animalia
bats
bat
(Uploaded by Plazi for the Bat Literature Project) Thirty-five Escherichia coli isolates obtained from the liver, spleen and intestines of 180 frugivorous and insec­ tivorous bats were investigated for antimicrobial resistance phenotypes/genotypes, prevalence of ExtendedSpectrum beta-lactamase (ESBL) production, virulence gene detection and molecular typing. Eight (22.9 %) of the isolates were multidrug resistant (MDR). Two isolates were cefotaxime-resistant, ESBL-producers and harbored the blaCTX-M-15 gene; they belonged to ST10184-D and ST2178-B1 lineages. tet(A) gene was detected in all tetracycline-resistant isolates while int1 (n = 8) and blaTEM (n = 7) genes were also found. Thirty-three of the E. coli isolates were assigned to seven phylogenetic groups, with B1 (45.7 %) being predominant. Three isolates were enteropathogenic E. coli (EPEC) pathovars, containing the eae gene (with the variants gamma and iota), and lacking stx1/stx2 genes. Bats in Nigeria are possible reservoirs of potentially pathogenic MDR E. coli isolates which may be important in the ecology of antimicrobial resistance at the human-livestock-wildlife-environment interfaces. The study reinforces the importance of including wildlife in national antimicrobial resistance moni­ toring programmes.
title Antimicrobial resistance in Escherichia coli isolates from frugivorous (Eidolon helvum) and insectivorous (Nycteris hispida) bats in Southeast Nigeria, with detection of CTX-M-15 producing isolates
topic Biodiversity
Mammalia
Chiroptera
Chordata
Animalia
bats
bat
url https://doi.org/10.5281/zenodo.13466534