Molecular characterisation of Toll-like receptors in the black flying fox Pteropus alecto

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Auteurs principaux: Cowled, Christopher, Baker, Michelle, Tachedjian, Mary, Zhou, Peng, Bulach, Dieter, Wang, Lin Fa
Format: Recurso digital
Publié: Zenodo 2011
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author Cowled, Christopher
Baker, Michelle
Tachedjian, Mary
Zhou, Peng
Bulach, Dieter
Wang, Lin Fa
author_facet Cowled, Christopher
Baker, Michelle
Tachedjian, Mary
Zhou, Peng
Bulach, Dieter
Wang, Lin Fa
contents (Uploaded by Plazi for the Bat Literature Project) Bats are believed to be reservoir hosts for a number of emerging and re-emerging viruses, many of which are responsible for illness and mortality in humans, livestock and other animals. In other vertebrates, early responses to viral infection involve engagement of Toll-like receptors (TLRs), which induce changes in gene expression collectively leading to an "antiviral state" In this study we report the cloning and bioinformatic analysis of a complete set of TLRs from the black flying fox Pteropus alecto, and perform quantitative tissue expression analysis of the nucleic acid-sensing TLRs 3, 7, 8 and 9. Full-length mRNA transcripts from TLRs homologous to human TLRs 1-10 were sequenced, as well as a nearly intact TLR13 pseudogene that was spliced and polyadenylated. This prototype data can now be used to design functional studies of the bat innate immune system. © 2010.
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publishDate 2011
publisher Zenodo
record_format zenodo
spellingShingle Molecular characterisation of Toll-like receptors in the black flying fox Pteropus alecto
Cowled, Christopher
Baker, Michelle
Tachedjian, Mary
Zhou, Peng
Bulach, Dieter
Wang, Lin Fa
Bat immunology
Chiroptera
Fruit bat
Innate immunity
Pteropus alecto
Toll-like receptor
Viral infection
Biodiversity
Mammalia
Chiroptera
Chordata
Animalia
bats
bat
(Uploaded by Plazi for the Bat Literature Project) Bats are believed to be reservoir hosts for a number of emerging and re-emerging viruses, many of which are responsible for illness and mortality in humans, livestock and other animals. In other vertebrates, early responses to viral infection involve engagement of Toll-like receptors (TLRs), which induce changes in gene expression collectively leading to an "antiviral state" In this study we report the cloning and bioinformatic analysis of a complete set of TLRs from the black flying fox Pteropus alecto, and perform quantitative tissue expression analysis of the nucleic acid-sensing TLRs 3, 7, 8 and 9. Full-length mRNA transcripts from TLRs homologous to human TLRs 1-10 were sequenced, as well as a nearly intact TLR13 pseudogene that was spliced and polyadenylated. This prototype data can now be used to design functional studies of the bat innate immune system. © 2010.
title Molecular characterisation of Toll-like receptors in the black flying fox Pteropus alecto
topic Bat immunology
Chiroptera
Fruit bat
Innate immunity
Pteropus alecto
Toll-like receptor
Viral infection
Biodiversity
Mammalia
Chiroptera
Chordata
Animalia
bats
bat
url https://doi.org/10.5281/zenodo.13527041