NODES_Inndiana_Z63_IN VITRO STUDY OF STORE-OPERATED CALCIUM ENTRY (SOCE) NEGATIVE MODULATORS ON VIABILITY, APOPTOSIS, AND CYTOKINE SECRETION OF HUMAN SYNOVIAL FIBROBLASTS
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| Natura: | Recurso digital |
| Lingua: | inglese |
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Zenodo
2024
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| _version_ | 1866901173630402560 |
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| author | Cappellano, Giuseppe |
| author_facet | Cappellano, Giuseppe |
| contents | <p>Osteoarthritis (OA) is a major cause of chronic pain and joint damage, involving low-grade synovitis that contributes to disease progression. Fibroblast-like synoviocytes (FLSs) are central to this process, producing inflammatory cytokines regulated by calcium (Ca2+) signaling. Targeting Ca2+ signaling through store-operated calcium entry (SOCE) could help reduce inflammation in OA.We tested two novel SOCE-negative modulators (CIC-39 and BIP-26) and two drugs (methotrexate and celecoxib) on inflamed OA-FLSs. CIC-39 and BIP-26 reduced Ca2+ influx without affecting cell morphology or viability. Methotrexate and the SOCE modulators showed trends toward reducing cytokine expression, though results were not statistically significant. Celecoxib induced cell death in a dose-dependent manner and caused cell cycle arrest. Overall, SOCE modulators show potential as a new treatment strategy for OA.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_13835778 |
| institution | Zenodo |
| language | eng |
| publishDate | 2024 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | NODES_Inndiana_Z63_IN VITRO STUDY OF STORE-OPERATED CALCIUM ENTRY (SOCE) NEGATIVE MODULATORS ON VIABILITY, APOPTOSIS, AND CYTOKINE SECRETION OF HUMAN SYNOVIAL FIBROBLASTS Cappellano, Giuseppe Spoke 5 - Industry for Health and Silver Economy INNDIANA Project Eco healthcare ECS00000036 <p>Osteoarthritis (OA) is a major cause of chronic pain and joint damage, involving low-grade synovitis that contributes to disease progression. Fibroblast-like synoviocytes (FLSs) are central to this process, producing inflammatory cytokines regulated by calcium (Ca2+) signaling. Targeting Ca2+ signaling through store-operated calcium entry (SOCE) could help reduce inflammation in OA.We tested two novel SOCE-negative modulators (CIC-39 and BIP-26) and two drugs (methotrexate and celecoxib) on inflamed OA-FLSs. CIC-39 and BIP-26 reduced Ca2+ influx without affecting cell morphology or viability. Methotrexate and the SOCE modulators showed trends toward reducing cytokine expression, though results were not statistically significant. Celecoxib induced cell death in a dose-dependent manner and caused cell cycle arrest. Overall, SOCE modulators show potential as a new treatment strategy for OA.</p> |
| title | NODES_Inndiana_Z63_IN VITRO STUDY OF STORE-OPERATED CALCIUM ENTRY (SOCE) NEGATIVE MODULATORS ON VIABILITY, APOPTOSIS, AND CYTOKINE SECRETION OF HUMAN SYNOVIAL FIBROBLASTS |
| topic | Spoke 5 - Industry for Health and Silver Economy INNDIANA Project Eco healthcare ECS00000036 |
| url | https://doi.org/10.5281/zenodo.13835778 |