Single-cell and in situ spatial analyses reveal the diversity of newly born hematopoietic stem cells and of their niches – Source data 6 relative to Fig. 6
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2024
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| author | Torcq, Léa Schmidt, Anne |
| author_facet | Torcq, Léa Schmidt, Anne |
| contents | <p>Raw image files (TIFF format) and segmented 3D images (.ims, Imaris proprietary files) relative to <strong>Fig.6C, G </strong>and <strong>Movie 5, 6</strong> (see <strong>Materials and Methods — </strong><strong>In situ gene expression analysis</strong>).</p> <p>The source data comprises z-stack acquisitions of 5dpf <em>Tg(runx1+23:eGFP)</em> or <em>Tg(kdrl:eGFP)</em> zebrafish larvae upper trunk regions, labelled with <em>gata2b</em> RNAscope <em>in situ</em> hybridization assay (n=6 individuals for <em>Tg(kdrl:eGFP)</em> and n=4 individuals for <em>Tg(runx1+23:eGFP)</em>). For all stacks, 2 fluorescence channels are displayed, corresponding to the cytoplasmic GFP expressed in hematopoietic/endothelial cells (green) and the <em>gata2b</em> RNAscope (magenta). Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.5 µm.</p> <p>The .ims files contain the 3D rendering of the z-stacks as well as the segmentations of <em>gata2b</em> RNAscope spots (spots 1: inside eGFP+ cells, spots all: all <em>gata2b</em> spots in and outside eGFP+ cells) and of hematopoietic/vascular cells (surface 1). For <em>Tg(kdrl:eGFP) </em> larvae, hematopoietic and vascular cells were classified depending on their proximity to major vascular structure (the aorta, the vein plexus or the SIA).</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_13884837 |
| institution | Zenodo |
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| publishDate | 2024 |
| publisher | Zenodo |
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| spellingShingle | Single-cell and in situ spatial analyses reveal the diversity of newly born hematopoietic stem cells and of their niches – Source data 6 relative to Fig. 6 Torcq, Léa Schmidt, Anne <p>Raw image files (TIFF format) and segmented 3D images (.ims, Imaris proprietary files) relative to <strong>Fig.6C, G </strong>and <strong>Movie 5, 6</strong> (see <strong>Materials and Methods — </strong><strong>In situ gene expression analysis</strong>).</p> <p>The source data comprises z-stack acquisitions of 5dpf <em>Tg(runx1+23:eGFP)</em> or <em>Tg(kdrl:eGFP)</em> zebrafish larvae upper trunk regions, labelled with <em>gata2b</em> RNAscope <em>in situ</em> hybridization assay (n=6 individuals for <em>Tg(kdrl:eGFP)</em> and n=4 individuals for <em>Tg(runx1+23:eGFP)</em>). For all stacks, 2 fluorescence channels are displayed, corresponding to the cytoplasmic GFP expressed in hematopoietic/endothelial cells (green) and the <em>gata2b</em> RNAscope (magenta). Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.5 µm.</p> <p>The .ims files contain the 3D rendering of the z-stacks as well as the segmentations of <em>gata2b</em> RNAscope spots (spots 1: inside eGFP+ cells, spots all: all <em>gata2b</em> spots in and outside eGFP+ cells) and of hematopoietic/vascular cells (surface 1). For <em>Tg(kdrl:eGFP) </em> larvae, hematopoietic and vascular cells were classified depending on their proximity to major vascular structure (the aorta, the vein plexus or the SIA).</p> |
| title | Single-cell and in situ spatial analyses reveal the diversity of newly born hematopoietic stem cells and of their niches – Source data 6 relative to Fig. 6 |
| url | https://doi.org/10.5281/zenodo.13884837 |