| _version_ | 1866901858927247360 |
|---|---|
| author | Pérez-Montaño, Francisco |
| author_facet | Pérez-Montaño, Francisco |
| contents | <p><em>Bromocresol Purple Lactose Broth is a special medium used to grow and detect fecal coliforms, </em><em>indicators of fecal contamination. Coliforms are the only bacteria that ferment lactose and </em><em>concomitantly produce high concentrations of acid and gas (mostly carbon dioxide and </em><em>hydrogen). To carry out the test, we need to have a nutrient medium that allows the bacteria to </em><em>grow, and a means of detecting acid and gas production. To detect gas production, the culture </em><em>tube contains a much smaller inverted tube – the Durham vial – which collects the gas, which </em><em>we can see as a bubble at the top of the tube. To detect the production of acid, the medium </em><em>contains a so-called acidity (pH) indictor, in our case bromocresol purple, which at normal pH </em><em>is purple, but at acid pH turns yellow. Thus, if fecal coliforms are present in the sample we take, </em><em>they will grow in the medium, change its color to yellow, and produce a gas bubble at the top of </em><em>the Durham vial.</em></p> <p> </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_14727469 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Bromocresol Purple Lactose Broth Pérez-Montaño, Francisco <p><em>Bromocresol Purple Lactose Broth is a special medium used to grow and detect fecal coliforms, </em><em>indicators of fecal contamination. Coliforms are the only bacteria that ferment lactose and </em><em>concomitantly produce high concentrations of acid and gas (mostly carbon dioxide and </em><em>hydrogen). To carry out the test, we need to have a nutrient medium that allows the bacteria to </em><em>grow, and a means of detecting acid and gas production. To detect gas production, the culture </em><em>tube contains a much smaller inverted tube – the Durham vial – which collects the gas, which </em><em>we can see as a bubble at the top of the tube. To detect the production of acid, the medium </em><em>contains a so-called acidity (pH) indictor, in our case bromocresol purple, which at normal pH </em><em>is purple, but at acid pH turns yellow. Thus, if fecal coliforms are present in the sample we take, </em><em>they will grow in the medium, change its color to yellow, and produce a gas bubble at the top of </em><em>the Durham vial.</em></p> <p> </p> |
| title | Bromocresol Purple Lactose Broth |
| url | https://doi.org/10.5281/zenodo.14727469 |