MIBIREM Deliverable 2.3 Metagenome and transcriptome of the six most promising microbiomes
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2024
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| _version_ | 1866901870195245056 |
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| author | French National Center for Scientific Research (head office) |
| author_facet | French National Center for Scientific Research (head office) |
| contents | <p>This deliverable describes the sequencing of metagenomes and metatranscriptomes of the most promising<br>microbiomes with efficient degrading activities for the three use cases. These data (currently under<br>acquisition) will be analysed to: i) reconstruct the genomes of each individual bacteria from the consortia<br>(MAGs), ii) predict their biochemical pathways and potential role in the consortia, and iii) identify the genes<br>and pathways that are over-expressed or repressed when the consortia are grown on contaminants and<br>during degradation. The metagenome and transcriptome were planned on the six most promising<br>microbiomes, but were actually performed on ten.<br>This information will be used to select the best microbiomes for the next steps, and mainly for directed<br>evolution. In addition, these data will be further used to target functional genes in bioaugmentation and<br>bioremediation assays during pilot and field testing of our microbiomes.<br>As a complement, ongoing actions will result in SIP-metabarcoding and metagenomic datasets that will be<br>included in an updated version of the deliverable prior to M36.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_14864574 |
| institution | Zenodo |
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| publishDate | 2024 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | MIBIREM Deliverable 2.3 Metagenome and transcriptome of the six most promising microbiomes French National Center for Scientific Research (head office) <p>This deliverable describes the sequencing of metagenomes and metatranscriptomes of the most promising<br>microbiomes with efficient degrading activities for the three use cases. These data (currently under<br>acquisition) will be analysed to: i) reconstruct the genomes of each individual bacteria from the consortia<br>(MAGs), ii) predict their biochemical pathways and potential role in the consortia, and iii) identify the genes<br>and pathways that are over-expressed or repressed when the consortia are grown on contaminants and<br>during degradation. The metagenome and transcriptome were planned on the six most promising<br>microbiomes, but were actually performed on ten.<br>This information will be used to select the best microbiomes for the next steps, and mainly for directed<br>evolution. In addition, these data will be further used to target functional genes in bioaugmentation and<br>bioremediation assays during pilot and field testing of our microbiomes.<br>As a complement, ongoing actions will result in SIP-metabarcoding and metagenomic datasets that will be<br>included in an updated version of the deliverable prior to M36.</p> |
| title | MIBIREM Deliverable 2.3 Metagenome and transcriptome of the six most promising microbiomes |
| url | https://doi.org/10.5281/zenodo.14864574 |