Cytotoxic Test Extract Ethanol Black Sea Cucumber ( Holothuria atra ) Against Line Cells HSC -3 in General In Vitro
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Zenodo
2025
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| _version_ | 1866901903776940032 |
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| author | Alma Tasia Utmi Arma Abu Bakar Yenita Alamsyah Dina Auliya Amly |
| author_facet | Alma Tasia Utmi Arma Abu Bakar Yenita Alamsyah Dina Auliya Amly |
| contents | <p>Oral Squamous Cell Carcinoma (OSCC) is one of the most aggressive malignant tumors due to its tendency to spread and high recurrence rate. Sea cucumbers have compounds that show anti- microbial, anti- inflammatory, and anticancer effects. The aim of this study was to determine the anticancer activity extract ethanol black sea cucumber ( Holothuria) atra ) against HSC-3 cell line in vitro. Extract black sea cucumber ( Holothuria) atra ) is done with the type of extraction maceration, then continued with u test Cytotoxicity by MTT Assay method using extract ethanol black sea cucumber ( Holothuria) atra ) at concentrations of 240 μg / ml, 120 μg / ml, 100 μg / ml, 80 μg / ml, 40 μg / ml, 20 μg / ml, 10 μg / ml, 5 μg / ml. At a concentration of 240 μg / ml has a cell viability of 35.215%, doxorubicin has a cell viability of 66.518%. The conclusion of this study is that there is cytotoxicity of ethanol extract of black sea cucumber ( Holothuria atra ) against HSC-3 cell line with IC50 value obtained of 41,371 μ g/ml using ethanol extract of Holothuria atra with concentration of 240 μ g/ml, 120 μ g/ml, 100 μ g/ml, 80 μ g/ml, 40 μ g/ml, 20 μ g/ml, 10 μ g/ml, 5 μ g/ml. At a concentration of 240 μg /ml there is a cell viability of 35,215%, this value is in the moderate toxicity category compared to the positive control of doxorubicin 0.1 μg /ml with a cell viability of 66,518% so that the extract ethanol black sea cucumber (Holothuria) atra ) has the potential to be an alternative to anticancer drugs.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_14881333 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Cytotoxic Test Extract Ethanol Black Sea Cucumber ( Holothuria atra ) Against Line Cells HSC -3 in General In Vitro Alma Tasia Utmi Arma Abu Bakar Yenita Alamsyah Dina Auliya Amly <p>Oral Squamous Cell Carcinoma (OSCC) is one of the most aggressive malignant tumors due to its tendency to spread and high recurrence rate. Sea cucumbers have compounds that show anti- microbial, anti- inflammatory, and anticancer effects. The aim of this study was to determine the anticancer activity extract ethanol black sea cucumber ( Holothuria) atra ) against HSC-3 cell line in vitro. Extract black sea cucumber ( Holothuria) atra ) is done with the type of extraction maceration, then continued with u test Cytotoxicity by MTT Assay method using extract ethanol black sea cucumber ( Holothuria) atra ) at concentrations of 240 μg / ml, 120 μg / ml, 100 μg / ml, 80 μg / ml, 40 μg / ml, 20 μg / ml, 10 μg / ml, 5 μg / ml. At a concentration of 240 μg / ml has a cell viability of 35.215%, doxorubicin has a cell viability of 66.518%. The conclusion of this study is that there is cytotoxicity of ethanol extract of black sea cucumber ( Holothuria atra ) against HSC-3 cell line with IC50 value obtained of 41,371 μ g/ml using ethanol extract of Holothuria atra with concentration of 240 μ g/ml, 120 μ g/ml, 100 μ g/ml, 80 μ g/ml, 40 μ g/ml, 20 μ g/ml, 10 μ g/ml, 5 μ g/ml. At a concentration of 240 μg /ml there is a cell viability of 35,215%, this value is in the moderate toxicity category compared to the positive control of doxorubicin 0.1 μg /ml with a cell viability of 66,518% so that the extract ethanol black sea cucumber (Holothuria) atra ) has the potential to be an alternative to anticancer drugs.</p> |
| title | Cytotoxic Test Extract Ethanol Black Sea Cucumber ( Holothuria atra ) Against Line Cells HSC -3 in General In Vitro |
| url | https://doi.org/10.5281/zenodo.14881333 |