Pichia ureolytica L. C. Guo, Y. J. Shang, F. Y. Bai & P. J. Han 2025, sp. nov.
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2025
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| author | Guo, Liang-Chen Hu, Shuang Zhu, Hai-Yan Shang, Yu-Jie Qiu, Yan-Jie Wen, Zhang Chen, Shen-Xi Bai, Feng-Yan Han, Pei-Jie |
| author_facet | Guo, Liang-Chen Hu, Shuang Zhu, Hai-Yan Shang, Yu-Jie Qiu, Yan-Jie Wen, Zhang Chen, Shen-Xi Bai, Feng-Yan Han, Pei-Jie |
| contents | <p><i>Pichia ureolytica</i> L. C. Guo, Y. J. Shang, F. Y. Bai & P. J. Han sp. nov.</p><p><b>Etymology.</b></p><p>The specific epithet <i>ureolytica</i> (u. re. o. ly’ti. ca. L. fem. adj.) derived from “ urea ” (the compound that can be broken down) and “ lyticus, ” which indicates the ability to break down or decompose urea.</p><p><b>Type.</b></p><p>The holotype CGMCC 2.6825 (original number = 3 L- 19-1) was isolated from bark of <i>Quercus wutaishansea</i> collected from Mentougou district, Beijing city, China (39.866 ° N; 115.598 ° E; warm temperate semi-humid and semi-arid monsoon climate) by Y. J. Shang in August 2021 and had been deposited in a metabolically inactive state in the China General Microbiological Culture Collection Centre (CGMCC), Beijing, China. The ex-type culture had been deposited in the Japan Collection of Microorganisms (JCM), Koyadai, Japan, as JCM 36370. GenBank accessions: ITS - PQ 586091 and LSU - PQ 586297.</p><p><b>Culture characteristics.</b></p><p>After growth on YPD agar for 3 days at 25 ° C, colonies are white, circular, butyrous and smooth with entire margins (Fig. 4 A). Cells are ovoid (3.5–5.0 × 3.8–5.2 μm) and occur singly or in pairs. Budding is multilateral and pseudohyphae are not formed (Fig. 4 B). Asci (4.7–5.4 × 5.5–6.2 μm) are persistent, typically forming four spherical spores within a diamond-shaped or subrounded ascus. (Fig. 4 C). The sexual structures were observed on YCB agar after 30 days at 25 ° C.</p><p><b>Physiological and biochemical characteristics.</b></p><p>Glucose fermentation is weak. Glucose, ethanol, succinic acid (slow / weak) and DL-lactic acid are assimilated as sole carbon sources. Galactose, L-sorbose, D-xylose, D-glucosamine, glycerol, glucitol, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, N-acetyl-D-glucosamine, L-arabinose, D-arabinose, xylitol, D-ribose, L-rhamnose, methanol, erythritol, ribitol, galactitol, D-mannitol, α-methyl-D-glucoside, salicin, D-glucuronic acid, sodium citrate dihydrate, inositol and hexadecane are not assimilated as sole carbon sources. Ethylamine hydrochloride, cadaverine dihydrochloride, L-lysine and ammonium sulfate are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated as sole nitrogen sources. Growth in vitaminfree medium and on 50 % and 60 % (w / v) glucose are positive, while growth in 10 % NaCl plus 5 % glucose medium is negative. Urease activity is positive (Suppl. material 4). Diazonium blue B reaction and production of extracellular starchlike compounds are negative. Growth on YPD agar at 30 ° C is positive, but negative at 37 ° C.</p><p><b>Notes.</b></p><p><i>Pichia ureolytica</i> is physiologically differentiated from its closely related species <i>Pichia paraexigua</i> and <i>Pichia scutulata</i> in terms of urease activity; from its closely related species <i>Pichia bovicola</i> and <i>Pichia phayaonensis</i> by growth on 60 % (w / v) glucose; from its closely related species <i>Pichia exigua</i> and <i>Pichia occidentalis</i> by the fact that <i>Pichia exigua</i> and <i>Pichia occidentalis</i> are capable of growth at 37 ° C, whereas <i>Pichia ureolytica</i> does not show growth under the same temperature conditions.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_14931675 |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | Pichia ureolytica L. C. Guo, Y. J. Shang, F. Y. Bai & P. J. Han 2025, sp. nov. Guo, Liang-Chen Hu, Shuang Zhu, Hai-Yan Shang, Yu-Jie Qiu, Yan-Jie Wen, Zhang Chen, Shen-Xi Bai, Feng-Yan Han, Pei-Jie Biodiversity Taxonomy Fungi Ascomycota Saccharomycetes Pichiales Pichiaceae Pichia Pichia ureolytica <p><i>Pichia ureolytica</i> L. C. Guo, Y. J. Shang, F. Y. Bai & P. J. Han sp. nov.</p><p><b>Etymology.</b></p><p>The specific epithet <i>ureolytica</i> (u. re. o. ly’ti. ca. L. fem. adj.) derived from “ urea ” (the compound that can be broken down) and “ lyticus, ” which indicates the ability to break down or decompose urea.</p><p><b>Type.</b></p><p>The holotype CGMCC 2.6825 (original number = 3 L- 19-1) was isolated from bark of <i>Quercus wutaishansea</i> collected from Mentougou district, Beijing city, China (39.866 ° N; 115.598 ° E; warm temperate semi-humid and semi-arid monsoon climate) by Y. J. Shang in August 2021 and had been deposited in a metabolically inactive state in the China General Microbiological Culture Collection Centre (CGMCC), Beijing, China. The ex-type culture had been deposited in the Japan Collection of Microorganisms (JCM), Koyadai, Japan, as JCM 36370. GenBank accessions: ITS - PQ 586091 and LSU - PQ 586297.</p><p><b>Culture characteristics.</b></p><p>After growth on YPD agar for 3 days at 25 ° C, colonies are white, circular, butyrous and smooth with entire margins (Fig. 4 A). Cells are ovoid (3.5–5.0 × 3.8–5.2 μm) and occur singly or in pairs. Budding is multilateral and pseudohyphae are not formed (Fig. 4 B). Asci (4.7–5.4 × 5.5–6.2 μm) are persistent, typically forming four spherical spores within a diamond-shaped or subrounded ascus. (Fig. 4 C). The sexual structures were observed on YCB agar after 30 days at 25 ° C.</p><p><b>Physiological and biochemical characteristics.</b></p><p>Glucose fermentation is weak. Glucose, ethanol, succinic acid (slow / weak) and DL-lactic acid are assimilated as sole carbon sources. Galactose, L-sorbose, D-xylose, D-glucosamine, glycerol, glucitol, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, N-acetyl-D-glucosamine, L-arabinose, D-arabinose, xylitol, D-ribose, L-rhamnose, methanol, erythritol, ribitol, galactitol, D-mannitol, α-methyl-D-glucoside, salicin, D-glucuronic acid, sodium citrate dihydrate, inositol and hexadecane are not assimilated as sole carbon sources. Ethylamine hydrochloride, cadaverine dihydrochloride, L-lysine and ammonium sulfate are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated as sole nitrogen sources. Growth in vitaminfree medium and on 50 % and 60 % (w / v) glucose are positive, while growth in 10 % NaCl plus 5 % glucose medium is negative. Urease activity is positive (Suppl. material 4). Diazonium blue B reaction and production of extracellular starchlike compounds are negative. Growth on YPD agar at 30 ° C is positive, but negative at 37 ° C.</p><p><b>Notes.</b></p><p><i>Pichia ureolytica</i> is physiologically differentiated from its closely related species <i>Pichia paraexigua</i> and <i>Pichia scutulata</i> in terms of urease activity; from its closely related species <i>Pichia bovicola</i> and <i>Pichia phayaonensis</i> by growth on 60 % (w / v) glucose; from its closely related species <i>Pichia exigua</i> and <i>Pichia occidentalis</i> by the fact that <i>Pichia exigua</i> and <i>Pichia occidentalis</i> are capable of growth at 37 ° C, whereas <i>Pichia ureolytica</i> does not show growth under the same temperature conditions.</p> |
| title | Pichia ureolytica L. C. Guo, Y. J. Shang, F. Y. Bai & P. J. Han 2025, sp. nov. |
| topic | Biodiversity Taxonomy Fungi Ascomycota Saccharomycetes Pichiales Pichiaceae Pichia Pichia ureolytica |
| url | https://doi.org/10.5281/zenodo.14931675 |