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| Language: | English |
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2025
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| Online Access: | https://doi.org/10.5281/zenodo.14993435 |
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| author | Fan, wenxin Xiao, Wupeng Xu, Chao Xu, Zengchao Liu, Yao Li, Weinan Guo, Jiayu Xue, Chengwen Chen, Jixin Liu, Xin Huang, Bangqin |
| author_facet | Fan, wenxin Xiao, Wupeng Xu, Chao Xu, Zengchao Liu, Yao Li, Weinan Guo, Jiayu Xue, Chengwen Chen, Jixin Liu, Xin Huang, Bangqin |
| contents | <p>Two cruises were conducted: the first in the Western Pacific (WP) aboard the R/V Kexue from 2 December 2021 to 3 January 2022, and the second in the South China Sea (SCS) aboard the R/V Tan Kah Kee from 28 November to 23 December 2022. A total of 221 samples were collected from 26 sites, spanning latitudes from 8°N to 22°N and longitudes from 111.5°E to 132.5°E, at discrete water depths ranging from the surface (5 m) to 1000 m in the mesopelagic zone. The data includes the bottom depth, depth, temperature, salinity, nutrient concentrations (nitrate plus nitrite: NOx, phosphate: PO₄³⁻, silicate: Si(OH)₄), chlorophyll <em>a</em> (Chl-<em>a</em>), bacterial abundance, bacterial production (BP), bacterial respiration (BR), bacterial carbon demand (BCD), net primary production (NPP), and dissolved organic carbon (DOC). Seawater temperature, and salinity were measured using a conductivity‐temperature‐depth (CTD) probe (SBE 911, Sea‐Bird Electronics) attached to a rosette sampler. The nutrient concentrations analyzed using a nutrient analyzer (QUATTRO, Seal, Germany) (Du et al., 2013). The Chl-<em>a</em> was measured by high‐performance liquid chromatography (HPLC) (Xiao et al., 2018a; Xiao et al., 2018b). Bacterial abundance was later assessed in the laboratory using a flow cytometer (Accuri C6) (Jiao et al., 2014). Rates of BP were determined through the uptake of ³H-leucine following the JGOFS protocol (Knap et al., 1996). BR was estimated by measuring the reduction of the tetrazolium salt INT (2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyltetrazolium chloride) to INT-formazan (INT-F) by Electron Transport System (ETS) dehydrogenase enzymes (Martínez‐García et al., 2009). BCD was calculated as the sum of BP and BR (García-Martín et al., 2019). Weekly NPP, a metric carbon export potential from the photic layer (Emerson, 2014), was estimated using Vertically Generalized Productivity Model (VGPM) products, available from the Ocean Productivity database (http://orca.science.oregonstate.edu/index.php/). DOC was analyzed according to high-temperature catalytic oxidation using a Shimadzu TOC-VCPH (Li et al., 2018).</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_14993435 |
| institution | Zenodo |
| language | eng |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Contrasting drivers of bacterial metabolism in the euphotic and mesopelagic zones of tropical oligotrophic oceans Fan, wenxin Xiao, Wupeng Xu, Chao Xu, Zengchao Liu, Yao Li, Weinan Guo, Jiayu Xue, Chengwen Chen, Jixin Liu, Xin Huang, Bangqin Bacterial carbon demand mesopelagic in vivo INT South China Sea Western Pacific <p>Two cruises were conducted: the first in the Western Pacific (WP) aboard the R/V Kexue from 2 December 2021 to 3 January 2022, and the second in the South China Sea (SCS) aboard the R/V Tan Kah Kee from 28 November to 23 December 2022. A total of 221 samples were collected from 26 sites, spanning latitudes from 8°N to 22°N and longitudes from 111.5°E to 132.5°E, at discrete water depths ranging from the surface (5 m) to 1000 m in the mesopelagic zone. The data includes the bottom depth, depth, temperature, salinity, nutrient concentrations (nitrate plus nitrite: NOx, phosphate: PO₄³⁻, silicate: Si(OH)₄), chlorophyll <em>a</em> (Chl-<em>a</em>), bacterial abundance, bacterial production (BP), bacterial respiration (BR), bacterial carbon demand (BCD), net primary production (NPP), and dissolved organic carbon (DOC). Seawater temperature, and salinity were measured using a conductivity‐temperature‐depth (CTD) probe (SBE 911, Sea‐Bird Electronics) attached to a rosette sampler. The nutrient concentrations analyzed using a nutrient analyzer (QUATTRO, Seal, Germany) (Du et al., 2013). The Chl-<em>a</em> was measured by high‐performance liquid chromatography (HPLC) (Xiao et al., 2018a; Xiao et al., 2018b). Bacterial abundance was later assessed in the laboratory using a flow cytometer (Accuri C6) (Jiao et al., 2014). Rates of BP were determined through the uptake of ³H-leucine following the JGOFS protocol (Knap et al., 1996). BR was estimated by measuring the reduction of the tetrazolium salt INT (2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyltetrazolium chloride) to INT-formazan (INT-F) by Electron Transport System (ETS) dehydrogenase enzymes (Martínez‐García et al., 2009). BCD was calculated as the sum of BP and BR (García-Martín et al., 2019). Weekly NPP, a metric carbon export potential from the photic layer (Emerson, 2014), was estimated using Vertically Generalized Productivity Model (VGPM) products, available from the Ocean Productivity database (http://orca.science.oregonstate.edu/index.php/). DOC was analyzed according to high-temperature catalytic oxidation using a Shimadzu TOC-VCPH (Li et al., 2018).</p> |
| title | Contrasting drivers of bacterial metabolism in the euphotic and mesopelagic zones of tropical oligotrophic oceans |
| topic | Bacterial carbon demand mesopelagic in vivo INT South China Sea Western Pacific |
| url | https://doi.org/10.5281/zenodo.14993435 |