Dataset related to article "Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells"
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| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Recurso digital |
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Zenodo
2025
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| _version_ | 1866901519463350272 |
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| author | Carnevale, Silvia Ponzetta, Andrea Rigatelli, Anna Carriero, Roberta Puccio, Simone Supino, Domenico Grieco, Giovanna Molisso, Piera Di Ceglie, Irene Scavello, Francesco Perucchini, Chiara Pasqualini, Fabio Recordati, Camilla Tripodo, Claudio Belmonte, Beatrice Mariancini, Andrea Kunderfranco, Paolo Sciumè, Giuseppe Lugli, Enrico Bonavita, Eduardo Magrini, Elena Garlanda, Cecilia Mantovani, Alberto Jaillon, Sebastien |
| author_facet | Carnevale, Silvia Ponzetta, Andrea Rigatelli, Anna Carriero, Roberta Puccio, Simone Supino, Domenico Grieco, Giovanna Molisso, Piera Di Ceglie, Irene Scavello, Francesco Perucchini, Chiara Pasqualini, Fabio Recordati, Camilla Tripodo, Claudio Belmonte, Beatrice Mariancini, Andrea Kunderfranco, Paolo Sciumè, Giuseppe Lugli, Enrico Bonavita, Eduardo Magrini, Elena Garlanda, Cecilia Mantovani, Alberto Jaillon, Sebastien |
| contents | <p>Neutrophils are the most abundant leukocytes in human blood and play a primary role in resistance against invading microorganisms and in the acute inflammatory response. However, their role in colitis and colitis-associated colorectal cancer is still under debate. This study aims to dissect the role of neutrophils in these pathologic contexts by using a rigorous genetic approach. Neutrophil-deficient mice (Csf3r-/- mice) were used in classic models of colitis and colitis-associated colorectal cancer and the role of neutrophils was assessed by histologic, cellular, and molecular analyses coupled with adoptive cell transfer. We also performed correlative analyses using human datasets. Csf3r-/- mice showed increased susceptibility to colitis and colitis-associated colorectal cancer compared with control Csf3r+/+ mice and adoptive transfer of neutrophils in Csf3r-/- mice reverted the phenotype. In colitis, Csf3r-/- mice showed increased bacterial invasion and a reduced number of healing ulcers in the colon, indicating a compromised regenerative capacity of epithelial cells. Neutrophils were essential for γδ T-cell polarization and IL22 production. In patients with ulcerative colitis, expression of CSF3R was positively correlated with IL22 and IL23 expression. Moreover, gene signatures associated with epithelial-cell development, proliferation, and antimicrobial response were enriched in CSF3Rhigh patients. Our data support a model where neutrophils mediate protection against intestinal inflammation and colitis-associated colorectal cancer by controlling the intestinal microbiota and driving the activation of an IL22-dependent tissue repair pathway. </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_15013654 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Dataset related to article "Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells" Carnevale, Silvia Ponzetta, Andrea Rigatelli, Anna Carriero, Roberta Puccio, Simone Supino, Domenico Grieco, Giovanna Molisso, Piera Di Ceglie, Irene Scavello, Francesco Perucchini, Chiara Pasqualini, Fabio Recordati, Camilla Tripodo, Claudio Belmonte, Beatrice Mariancini, Andrea Kunderfranco, Paolo Sciumè, Giuseppe Lugli, Enrico Bonavita, Eduardo Magrini, Elena Garlanda, Cecilia Mantovani, Alberto Jaillon, Sebastien <p>Neutrophils are the most abundant leukocytes in human blood and play a primary role in resistance against invading microorganisms and in the acute inflammatory response. However, their role in colitis and colitis-associated colorectal cancer is still under debate. This study aims to dissect the role of neutrophils in these pathologic contexts by using a rigorous genetic approach. Neutrophil-deficient mice (Csf3r-/- mice) were used in classic models of colitis and colitis-associated colorectal cancer and the role of neutrophils was assessed by histologic, cellular, and molecular analyses coupled with adoptive cell transfer. We also performed correlative analyses using human datasets. Csf3r-/- mice showed increased susceptibility to colitis and colitis-associated colorectal cancer compared with control Csf3r+/+ mice and adoptive transfer of neutrophils in Csf3r-/- mice reverted the phenotype. In colitis, Csf3r-/- mice showed increased bacterial invasion and a reduced number of healing ulcers in the colon, indicating a compromised regenerative capacity of epithelial cells. Neutrophils were essential for γδ T-cell polarization and IL22 production. In patients with ulcerative colitis, expression of CSF3R was positively correlated with IL22 and IL23 expression. Moreover, gene signatures associated with epithelial-cell development, proliferation, and antimicrobial response were enriched in CSF3Rhigh patients. Our data support a model where neutrophils mediate protection against intestinal inflammation and colitis-associated colorectal cancer by controlling the intestinal microbiota and driving the activation of an IL22-dependent tissue repair pathway. </p> |
| title | Dataset related to article "Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells" |
| url | https://doi.org/10.5281/zenodo.15013654 |