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2025
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| Online Access: | https://doi.org/10.5281/zenodo.15054613 |
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| _version_ | 1866902042911440896 |
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| author | Ghaly, Timothy |
| author_facet | Ghaly, Timothy |
| contents | <p>Metagenome-assembled genome (MAG) sequences from the global soil microbiome were obtained from the <a href="https://doi.org/10.1038/s41467-023-43000-z" target="_blank" rel="noopener">Soil Microbial Dark Matter MAG</a> and <a href="https://doi.org/10.1101/2024.12.19.629313" target="_blank" rel="noopener">Microflora Danica long-read MAG</a> catalogues, and filtered to retain only high-quality (HQ) MAGs based on <a href="https://doi.org/10.1038/nbt.3893" target="_blank" rel="noopener">MIMAG</a> standards. That is, genomes that had >90% CheckM2 completeness and <5% CheckM2 contamination, and had the 23S, 16S, and 5S rRNA genes, and at least 18 different tRNA genes. MAGs were dereplicated at approximately species-level, using clustering thresholds of 95% average nucleotide identity (ANI) and 50% aligned fraction (AF).<br><br>MAG sequences are within the compressed directory, 'MAGs.tar.gz'.<br>Pooled predicted protein sequences are in 'HQ_MAGs.prots.faa.gz'.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_15054613 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | 8,163 High-Quality metagenome-assembled genomes from the global soil microbiome Ghaly, Timothy <p>Metagenome-assembled genome (MAG) sequences from the global soil microbiome were obtained from the <a href="https://doi.org/10.1038/s41467-023-43000-z" target="_blank" rel="noopener">Soil Microbial Dark Matter MAG</a> and <a href="https://doi.org/10.1101/2024.12.19.629313" target="_blank" rel="noopener">Microflora Danica long-read MAG</a> catalogues, and filtered to retain only high-quality (HQ) MAGs based on <a href="https://doi.org/10.1038/nbt.3893" target="_blank" rel="noopener">MIMAG</a> standards. That is, genomes that had >90% CheckM2 completeness and <5% CheckM2 contamination, and had the 23S, 16S, and 5S rRNA genes, and at least 18 different tRNA genes. MAGs were dereplicated at approximately species-level, using clustering thresholds of 95% average nucleotide identity (ANI) and 50% aligned fraction (AF).<br><br>MAG sequences are within the compressed directory, 'MAGs.tar.gz'.<br>Pooled predicted protein sequences are in 'HQ_MAGs.prots.faa.gz'.</p> |
| title | 8,163 High-Quality metagenome-assembled genomes from the global soil microbiome |
| url | https://doi.org/10.5281/zenodo.15054613 |