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| Main Authors: | , |
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| Format: | Recurso digital |
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Zenodo
2021
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| Online Access: | https://doi.org/10.5281/zenodo.15138965 |
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Table of Contents:
- <p><b>Table 1.</b> Primers and protocols of polymerase chain reactions of every molecular marker.</p><table><thead><tr><th colspan="2" rowspan="1">Marker</th><th colspan="1" rowspan="1">Primer</th><th colspan="1" rowspan="1">Sequences (5 ‘-3‘)</th><th colspan="1" rowspan="1">PCR protocol</th><th colspan="1" rowspan="1">Reference</th></tr></thead><tbody><tr><td colspan="2" rowspan="2">12S</td><td colspan="1" rowspan="1">12S1a</td><td colspan="1" rowspan="1">TAAGTGCCAGCAGACGCGGT</td><td colspan="1" rowspan="2">(95 °C for 4 min) + 4 × [(94°C for 30 sec) → (50°C for 1 min) → (72°C for 2 min)]+30 × [(94°C for 30 sec) → (55°C for 1 min) → (72°C for 2 min)] + (72°C for 4 min)</td><td colspan="1" rowspan="2">Sinniger et al. (2005)</td></tr><tr><td colspan="1" rowspan="1">12S3r</td><td colspan="1" rowspan="1">ACGGGCAATTTGTACTAACA</td></tr><tr><td colspan="2" rowspan="4">16S</td><td colspan="1" rowspan="1">ANEM16SA</td><td colspan="1" rowspan="1">CACTGACCGTGATAATGTAGCGT</td><td colspan="1" rowspan="2">(95°C for 4 min) + 30 × [(95°C for 30 sec) → (46°C for 45 sec) → (72°C for 1 min)] + (72°C for 5 min)</td><td colspan="1" rowspan="2">Geller and Walton (2001)</td></tr><tr><td colspan="1" rowspan="1">ANEM16SB</td><td colspan="1" rowspan="1">CCCCATGGTAGCTTTTATTCG</td></tr><tr><td colspan="1" rowspan="1">16Sant1a</td><td colspan="1" rowspan="1">GCCATGAGTATAGACGCACA</td><td colspan="1" rowspan="2">(95°C for 4 min) + 30 × [(95°C for 30 sec) → (46°C for 45 sec) → (72°C for 1 min)] + (72°C for 5 min)</td><td colspan="1" rowspan="2">Sinniger et al. (2005)</td></tr><tr><td colspan="1" rowspan="1">16SbmoH</td><td colspan="1" rowspan="1">CGAACAGCCAACCCTTGG</td></tr><tr><td colspan="1" rowspan="6">18S</td><td colspan="1" rowspan="2">PCR</td><td colspan="1" rowspan="1">18SA</td><td colspan="1" rowspan="1">AACCTGGTTGATCCTGCCAGT</td><td colspan="1" rowspan="2">(94°C for 4 min) + 35 × [(94°C for 20 sec) → (57°C for 20 sec) → (72°C for 1 min 45 sec)] + (72°C for 7 min)</td><td colspan="1" rowspan="6">Medlin et al. (1988) Apakupakul et al. (1999)</td></tr><tr><td colspan="1" rowspan="1">18SB</td><td colspan="1" rowspan="1">TGATCCTTCCGCAGGTTCACCT</td></tr><tr><td colspan="1" rowspan="4">Only sequence</td><td colspan="1" rowspan="1">18SL</td><td colspan="1" rowspan="1">CCAACTACGAGCTTTTTAACTG</td><td colspan="1" rowspan="4"></td></tr><tr><td colspan="1" rowspan="1">18SC</td><td colspan="1" rowspan="1">CGGTAATTCCAGCTCCAATAG</td></tr><tr><td colspan="1" rowspan="1">18SY</td><td colspan="1" rowspan="1">CAGACAAATCGCTCCACCAAC</td></tr><tr><td colspan="1" rowspan="1">18SO</td><td colspan="1" rowspan="1">AAGGGCACCACCAGGAGTGGAG</td></tr></tbody></table>