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. PCR primers used for both amplification and sequencing. The Dir column identifies forward (F) and reverse (R) primers and indicates external (ex) and internal (in) primers used for nested or hemi-nested PCR. The Ref column lists the reference for the original description of the primer: (1) Maddison 2008; (2) Moore and Robertson 2014; (3) Moulton and Wiegmann 2004; (4) Ober 2002; (5) Simon et al. 1994; (6) Van der Auwera et al. 1994; (7) Vogler and DeSalle 1993; (8) Ward and Downie 2005; (9) Wild and Maddison 2008. in Phylogeny of the supertribe Nebriitae (Coleoptera, Carabidae) based on analyses of DNA sequence data

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Hauptverfasser:	Kavanaugh, David H., Maddison, David R., Simison, W. Brian, Schoville, Sean D., Schmidt, Joachim, Faille, Arnaud, Moore, Wendy, Pflug, James M., Archambeault, Sophie L., Hoang, Tinya, Chen, Jei-Ying
Format:	Recurso digital
Veröffentlicht:	Zenodo 2021
Schlagworte:	Biodiversity Taxonomy
Online-Zugang:	Acceder al recurso
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_version_	1866901652361969664
author	Kavanaugh, David H. Maddison, David R. Simison, W. Brian Schoville, Sean D. Schmidt, Joachim Faille, Arnaud Moore, Wendy Pflug, James M. Archambeault, Sophie L. Hoang, Tinya Chen, Jei-Ying
author_facet	Kavanaugh, David H. Maddison, David R. Simison, W. Brian Schoville, Sean D. Schmidt, Joachim Faille, Arnaud Moore, Wendy Pflug, James M. Archambeault, Sophie L. Hoang, Tinya Chen, Jei-Ying
contents	<p><b>.</b> PCR primers used for both amplification and sequencing. The Dir column identifies forward (F) and reverse (R) primers and indicates external (ex) and internal (in) primers used for nested or hemi-nested PCR. The Ref column lists the reference for the original description of the primer: (1) Maddison 2008; (2) Moore and Robertson 2014; (3) Moulton and Wiegmann 2004; (4) Ober 2002; (5) Simon et al. 1994; (6) Van der Auwera et al. 1994; (7) Vogler and DeSalle 1993; (8) Ward and Downie 2005; (9) Wild and Maddison 2008.</p><table><thead><tr><th colspan="1" rowspan="1">Gene</th><th colspan="1" rowspan="1">Primer</th><th colspan="1" rowspan="1">Dir</th><th colspan="1" rowspan="1">Sequence</th><th colspan="1" rowspan="1">Ref</th></tr></thead><tbody><tr><td colspan="1" rowspan="4">28S</td><td colspan="1" rowspan="1">LS58</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GGGAGGAAAAGAAACTAAC</td><td colspan="1" rowspan="1">4</td></tr><tr><td colspan="1" rowspan="1">NLF184/21</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">ACCCGCTGAAYTTAAGCATAT</td><td colspan="1" rowspan="1">6</td></tr><tr><td colspan="1" rowspan="1">LS998</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GCATAGTTCACCATCTTTC</td><td colspan="1" rowspan="1">4</td></tr><tr><td colspan="1" rowspan="1">LS1041</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">TACGGACRTCCATCAGGGTTTCCCCTGACTTC</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="2">16S-ND1</td><td colspan="1" rowspan="1">16SAR</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">CGCCTGTTTAACAAAAACAT</td><td colspan="1" rowspan="1">7</td></tr><tr><td colspan="1" rowspan="1">ND1A</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GGTCCCTTACGAATTTGAATATATCCT</td><td colspan="1" rowspan="1">5</td></tr><tr><td colspan="1" rowspan="2">CO1 PJ</td><td colspan="1" rowspan="1">Jer</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">CAACATTTATTTTGATTTTTTGG</td><td colspan="1" rowspan="1">5</td></tr><tr><td colspan="1" rowspan="1">Pat</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">TCCAATGCACTAATCTGCCATATTA</td><td colspan="1" rowspan="1">5</td></tr><tr><td colspan="1" rowspan="2">CO1 BC</td><td colspan="1" rowspan="1">LCO1490</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GGTCAACAAATCATAAAGATATTGG</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="1">HCO2198</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">TAAACTTCAGGGTGACCAAAAAATCA</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="4"><i>wg</i></td><td colspan="1" rowspan="1"><i>wg</i> 550F</td><td colspan="1" rowspan="1">Fex</td><td colspan="1" rowspan="1">ATGCGTCAGGARTGYAARTGYCAYGGYATGTC</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1"><i>wg</i> 578F</td><td colspan="1" rowspan="1">Fin</td><td colspan="1" rowspan="1">TGCACNGTGAARACYTGCTGGATG</td><td colspan="1" rowspan="1">8</td></tr><tr><td colspan="1" rowspan="1"><i>wg</i> AbR</td><td colspan="1" rowspan="1">Rin</td><td colspan="1" rowspan="1">YTCGCAGCACCARTGGAA</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1"><i>wg</i> AbRZ</td><td colspan="1" rowspan="1">Rex</td><td colspan="1" rowspan="1">CACTTNACYTCRCARCACCARTG</td><td colspan="1" rowspan="1">8</td></tr><tr><td colspan="1" rowspan="3">CAD2</td><td colspan="1" rowspan="1">CD398F</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GARCAYACAGCNGGNCCNCAAGA</td><td colspan="1" rowspan="1">3</td></tr><tr><td colspan="1" rowspan="1">CD581F4</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GGWGGWCAAACTGCWYTMAAYTGYGG</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="1">CD696R</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">AANGGRTCNACRTTTTCCATATT</td><td colspan="1" rowspan="1">2</td></tr><tr><td colspan="1" rowspan="3">PEPCK</td><td colspan="1" rowspan="1">PK282</td><td colspan="1" rowspan="1">Fex</td><td colspan="1" rowspan="1">GAAGGATGGCTBGCNGARCAYATG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">PK330F</td><td colspan="1" rowspan="1">Fin</td><td colspan="1" rowspan="1">GAGGACCAAGCNGAYACNGTDGGTTGTTG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">PK485R</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GCAGCVGTNGCYTCRCTYCTCAT</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="3">Topo</td><td colspan="1" rowspan="1">TP643F</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GACGATTGGAARTCNAARGARATG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">TP675F</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GAGGACCAAGCNGAYACNGTDGGTTGTTG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">TP932R</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GGWCCDGCATCDATDGCCCA</td><td colspan="1" rowspan="1">9</td></tr></tbody></table>
format	Recurso digital
id	zenodo_https___doi_org_10_5281_zenodo_15139416
institution	Zenodo
language
publishDate	2021
publisher	Zenodo
record_format	zenodo
spellingShingle	. PCR primers used for both amplification and sequencing. The Dir column identifies forward (F) and reverse (R) primers and indicates external (ex) and internal (in) primers used for nested or hemi-nested PCR. The Ref column lists the reference for the original description of the primer: (1) Maddison 2008; (2) Moore and Robertson 2014; (3) Moulton and Wiegmann 2004; (4) Ober 2002; (5) Simon et al. 1994; (6) Van der Auwera et al. 1994; (7) Vogler and DeSalle 1993; (8) Ward and Downie 2005; (9) Wild and Maddison 2008. in Phylogeny of the supertribe Nebriitae (Coleoptera, Carabidae) based on analyses of DNA sequence data Kavanaugh, David H. Maddison, David R. Simison, W. Brian Schoville, Sean D. Schmidt, Joachim Faille, Arnaud Moore, Wendy Pflug, James M. Archambeault, Sophie L. Hoang, Tinya Chen, Jei-Ying Biodiversity Taxonomy <p><b>.</b> PCR primers used for both amplification and sequencing. The Dir column identifies forward (F) and reverse (R) primers and indicates external (ex) and internal (in) primers used for nested or hemi-nested PCR. The Ref column lists the reference for the original description of the primer: (1) Maddison 2008; (2) Moore and Robertson 2014; (3) Moulton and Wiegmann 2004; (4) Ober 2002; (5) Simon et al. 1994; (6) Van der Auwera et al. 1994; (7) Vogler and DeSalle 1993; (8) Ward and Downie 2005; (9) Wild and Maddison 2008.</p><table><thead><tr><th colspan="1" rowspan="1">Gene</th><th colspan="1" rowspan="1">Primer</th><th colspan="1" rowspan="1">Dir</th><th colspan="1" rowspan="1">Sequence</th><th colspan="1" rowspan="1">Ref</th></tr></thead><tbody><tr><td colspan="1" rowspan="4">28S</td><td colspan="1" rowspan="1">LS58</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GGGAGGAAAAGAAACTAAC</td><td colspan="1" rowspan="1">4</td></tr><tr><td colspan="1" rowspan="1">NLF184/21</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">ACCCGCTGAAYTTAAGCATAT</td><td colspan="1" rowspan="1">6</td></tr><tr><td colspan="1" rowspan="1">LS998</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GCATAGTTCACCATCTTTC</td><td colspan="1" rowspan="1">4</td></tr><tr><td colspan="1" rowspan="1">LS1041</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">TACGGACRTCCATCAGGGTTTCCCCTGACTTC</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="2">16S-ND1</td><td colspan="1" rowspan="1">16SAR</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">CGCCTGTTTAACAAAAACAT</td><td colspan="1" rowspan="1">7</td></tr><tr><td colspan="1" rowspan="1">ND1A</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GGTCCCTTACGAATTTGAATATATCCT</td><td colspan="1" rowspan="1">5</td></tr><tr><td colspan="1" rowspan="2">CO1 PJ</td><td colspan="1" rowspan="1">Jer</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">CAACATTTATTTTGATTTTTTGG</td><td colspan="1" rowspan="1">5</td></tr><tr><td colspan="1" rowspan="1">Pat</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">TCCAATGCACTAATCTGCCATATTA</td><td colspan="1" rowspan="1">5</td></tr><tr><td colspan="1" rowspan="2">CO1 BC</td><td colspan="1" rowspan="1">LCO1490</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GGTCAACAAATCATAAAGATATTGG</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="1">HCO2198</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">TAAACTTCAGGGTGACCAAAAAATCA</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="4"><i>wg</i></td><td colspan="1" rowspan="1"><i>wg</i> 550F</td><td colspan="1" rowspan="1">Fex</td><td colspan="1" rowspan="1">ATGCGTCAGGARTGYAARTGYCAYGGYATGTC</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1"><i>wg</i> 578F</td><td colspan="1" rowspan="1">Fin</td><td colspan="1" rowspan="1">TGCACNGTGAARACYTGCTGGATG</td><td colspan="1" rowspan="1">8</td></tr><tr><td colspan="1" rowspan="1"><i>wg</i> AbR</td><td colspan="1" rowspan="1">Rin</td><td colspan="1" rowspan="1">YTCGCAGCACCARTGGAA</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1"><i>wg</i> AbRZ</td><td colspan="1" rowspan="1">Rex</td><td colspan="1" rowspan="1">CACTTNACYTCRCARCACCARTG</td><td colspan="1" rowspan="1">8</td></tr><tr><td colspan="1" rowspan="3">CAD2</td><td colspan="1" rowspan="1">CD398F</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GARCAYACAGCNGGNCCNCAAGA</td><td colspan="1" rowspan="1">3</td></tr><tr><td colspan="1" rowspan="1">CD581F4</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GGWGGWCAAACTGCWYTMAAYTGYGG</td><td colspan="1" rowspan="1">1</td></tr><tr><td colspan="1" rowspan="1">CD696R</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">AANGGRTCNACRTTTTCCATATT</td><td colspan="1" rowspan="1">2</td></tr><tr><td colspan="1" rowspan="3">PEPCK</td><td colspan="1" rowspan="1">PK282</td><td colspan="1" rowspan="1">Fex</td><td colspan="1" rowspan="1">GAAGGATGGCTBGCNGARCAYATG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">PK330F</td><td colspan="1" rowspan="1">Fin</td><td colspan="1" rowspan="1">GAGGACCAAGCNGAYACNGTDGGTTGTTG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">PK485R</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GCAGCVGTNGCYTCRCTYCTCAT</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="3">Topo</td><td colspan="1" rowspan="1">TP643F</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GACGATTGGAARTCNAARGARATG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">TP675F</td><td colspan="1" rowspan="1">F</td><td colspan="1" rowspan="1">GAGGACCAAGCNGAYACNGTDGGTTGTTG</td><td colspan="1" rowspan="1">9</td></tr><tr><td colspan="1" rowspan="1">TP932R</td><td colspan="1" rowspan="1">R</td><td colspan="1" rowspan="1">GGWCCDGCATCDATDGCCCA</td><td colspan="1" rowspan="1">9</td></tr></tbody></table>
title	. PCR primers used for both amplification and sequencing. The Dir column identifies forward (F) and reverse (R) primers and indicates external (ex) and internal (in) primers used for nested or hemi-nested PCR. The Ref column lists the reference for the original description of the primer: (1) Maddison 2008; (2) Moore and Robertson 2014; (3) Moulton and Wiegmann 2004; (4) Ober 2002; (5) Simon et al. 1994; (6) Van der Auwera et al. 1994; (7) Vogler and DeSalle 1993; (8) Ward and Downie 2005; (9) Wild and Maddison 2008. in Phylogeny of the supertribe Nebriitae (Coleoptera, Carabidae) based on analyses of DNA sequence data
topic	Biodiversity Taxonomy
url	https://doi.org/10.5281/zenodo.15139416