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Table 2 h AChE, h in Amaryllidaceae alkaloids from Narcissus pseudonarcissus L. cv. Dutch Master as potential drugs in treatment of Alzheimer's disease

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Main Authors:	Hulcová, Daniela, Maříková, Jana, Korábečný, Jan, Hošťálková, Anna, Jun, Daniel, Kuneš, Jiří, Chlebek, Jakub, Opletal, Lubomír, Simone, Angela De, Nováková, Lucie, Andrisano, Vincenza, Růžička, Aleš, Cahlíková, Lucie
Format:	Recurso digital
Published:	Zenodo 2019
Subjects:	Biodiversity Taxonomy
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_version_	1866901910933471232
author	Hulcová, Daniela Maříková, Jana Korábečný, Jan Hošťálková, Anna Jun, Daniel Kuneš, Jiří Chlebek, Jakub Opletal, Lubomír Simone, Angela De Nováková, Lucie Andrisano, Vincenza Růžička, Aleš Cahlíková, Lucie
author_facet	Hulcová, Daniela Maříková, Jana Korábečný, Jan Hošťálková, Anna Jun, Daniel Kuneš, Jiří Chlebek, Jakub Opletal, Lubomír Simone, Angela De Nováková, Lucie Andrisano, Vincenza Růžička, Aleš Cahlíková, Lucie
contents	<p><b>Table 2</b> <i>h</i> AChE, <i>h</i> BuChE, POP and GSK-3β inhibitory activities of the tested Amaryllidaceae alkaloids isolated from <i>Narcissus pseudonarcissus</i> cv. Dutch Master expressed as IC50.</p><table><thead><tr><th>Compound</th><th><i>h</i> AChE IC (μM) a 50</th><th><i>h</i> BuChE IC (μM) a 50</th><th>SI for <i>h</i> AChE b</th><th>POP IC a (μM) 50</th><th>GSK-3β (% inhib.) c</th><th>GSK-3β IC a (μM) 50</th></tr></thead><tbody><tr><th>Homolycorine (1)</th><td>64 ± 4</td><td>151 ± 20</td><td>2.4</td><td>173 ± 41</td><td>54 ± 1</td><td>n.d.</td></tr><tr><th>Masonine (2)</th><td>305 ± 34</td><td>229 ± 24</td><td>0.8</td><td>314 ± 34</td><td>66 ± 4</td><td>27.9 ± 0.8</td></tr><tr><th>Seco-isopowellaminone (3)</th><td>294 ± 33</td><td>> 500</td><td>> 1.7</td><td>> 500</td><td>38 ± 1</td><td>n.d.</td></tr><tr><th><i>O</i> -Ethyllycorenine (4)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>58 ± 3</td><td>n.d.</td></tr><tr><th><i>N</i> -Demethylmasonine (5)</th><td>> 500</td><td>474 ± 13</td><td><0.9</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Narcimatuline (6)</th><td>489 ± 60</td><td>5.9 ± 0.2</td><td>0.01</td><td>29.2 ± 1.0</td><td>67 ± 3</td><td>20.7 ± 2.4</td></tr><tr><th><i>O</i> -Acetylpluviine (7)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Tazettine (8)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>49 ± 0</td><td>n.d.</td></tr><tr><th>Galanthine (9)</th><td>606 ± 60</td><td>> 500</td><td>> 1.7</td><td>> 500</td><td>26 ± 7</td><td>n.d.</td></tr><tr><th>Galanthamine (10)</th><td>1.7 ± 0.1</td><td>42.3 ± 1.3</td><td>24.9</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Narwedine (11)</th><td>281 ± 34</td><td>911 ± 69</td><td>3.2</td><td>907 ± 87</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Caranine (12)</th><td>321 ± 42</td><td>487 ± 55</td><td>1.5</td><td>> 1000</td><td>58 ± 9</td><td>30.8 ± 0.3</td></tr><tr><th>Lycoraminone (13)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>n.d.</td><td>39 ± 1</td><td>n.d.</td></tr><tr><th>Lycoramine (14)</th><td>456 ± 57</td><td>> 500</td><td>> 1.1</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th><i>O</i> -Methyllycorenine (15)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Lycorenine (16)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>48 ± 3</td><td>n.d.</td></tr><tr><th>Oduline (17)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>252 ± 27</td><td>58 ± 4</td><td>n.d.</td></tr><tr><th>Haemanthamine (18)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>52 ± 0</td><td>n.d.</td></tr><tr><th>Tetrahydromasonine (19)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>22 ± 0</td><td>n.d.</td></tr><tr><th>Hippeastrine (20)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>11 ± 2</td><td>n.d.</td></tr><tr><th>Crinine (21)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>40 ± 5</td><td>n.d.</td></tr><tr><th>Epimaritidine (22)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Huperzine A c</th><td>0.033 ± 0.001</td><td>> 500</td><td>> 15.151</td><td>n.d.</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Berberine c</th><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>142 ± 21</td><td>n.d.</td><td>n.d.</td></tr><tr><th><i>Z</i> -Pro-prolinal c</th><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>2.75 × 10−3</td><td>n.d.</td><td>n.d.</td></tr><tr><th>SB-415286 c</th><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>70 nM</td></tr></tbody></table><p><sup>a</sup> Compound concentration required to decrease enzyme activity by 50%; the values are the mean ± SEM of three independent measurements, each performed in triplicate; <sup>b</sup> Selectivity index (SI) for <i>h</i> AChE is determined as ratio <i>h</i> BuChE IC / <i>h</i> AChE IC; <sup>c</sup> tested at 10 μM compound concentration; n.d. stands for not determined.</p>
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spellingShingle	Table 2 h AChE, h in Amaryllidaceae alkaloids from Narcissus pseudonarcissus L. cv. Dutch Master as potential drugs in treatment of Alzheimer's disease Hulcová, Daniela Maříková, Jana Korábečný, Jan Hošťálková, Anna Jun, Daniel Kuneš, Jiří Chlebek, Jakub Opletal, Lubomír Simone, Angela De Nováková, Lucie Andrisano, Vincenza Růžička, Aleš Cahlíková, Lucie Biodiversity Taxonomy <p><b>Table 2</b> <i>h</i> AChE, <i>h</i> BuChE, POP and GSK-3β inhibitory activities of the tested Amaryllidaceae alkaloids isolated from <i>Narcissus pseudonarcissus</i> cv. Dutch Master expressed as IC50.</p><table><thead><tr><th>Compound</th><th><i>h</i> AChE IC (μM) a 50</th><th><i>h</i> BuChE IC (μM) a 50</th><th>SI for <i>h</i> AChE b</th><th>POP IC a (μM) 50</th><th>GSK-3β (% inhib.) c</th><th>GSK-3β IC a (μM) 50</th></tr></thead><tbody><tr><th>Homolycorine (1)</th><td>64 ± 4</td><td>151 ± 20</td><td>2.4</td><td>173 ± 41</td><td>54 ± 1</td><td>n.d.</td></tr><tr><th>Masonine (2)</th><td>305 ± 34</td><td>229 ± 24</td><td>0.8</td><td>314 ± 34</td><td>66 ± 4</td><td>27.9 ± 0.8</td></tr><tr><th>Seco-isopowellaminone (3)</th><td>294 ± 33</td><td>> 500</td><td>> 1.7</td><td>> 500</td><td>38 ± 1</td><td>n.d.</td></tr><tr><th><i>O</i> -Ethyllycorenine (4)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>58 ± 3</td><td>n.d.</td></tr><tr><th><i>N</i> -Demethylmasonine (5)</th><td>> 500</td><td>474 ± 13</td><td><0.9</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Narcimatuline (6)</th><td>489 ± 60</td><td>5.9 ± 0.2</td><td>0.01</td><td>29.2 ± 1.0</td><td>67 ± 3</td><td>20.7 ± 2.4</td></tr><tr><th><i>O</i> -Acetylpluviine (7)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Tazettine (8)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>49 ± 0</td><td>n.d.</td></tr><tr><th>Galanthine (9)</th><td>606 ± 60</td><td>> 500</td><td>> 1.7</td><td>> 500</td><td>26 ± 7</td><td>n.d.</td></tr><tr><th>Galanthamine (10)</th><td>1.7 ± 0.1</td><td>42.3 ± 1.3</td><td>24.9</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Narwedine (11)</th><td>281 ± 34</td><td>911 ± 69</td><td>3.2</td><td>907 ± 87</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Caranine (12)</th><td>321 ± 42</td><td>487 ± 55</td><td>1.5</td><td>> 1000</td><td>58 ± 9</td><td>30.8 ± 0.3</td></tr><tr><th>Lycoraminone (13)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>n.d.</td><td>39 ± 1</td><td>n.d.</td></tr><tr><th>Lycoramine (14)</th><td>456 ± 57</td><td>> 500</td><td>> 1.1</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th><i>O</i> -Methyllycorenine (15)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Lycorenine (16)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>48 ± 3</td><td>n.d.</td></tr><tr><th>Oduline (17)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>252 ± 27</td><td>58 ± 4</td><td>n.d.</td></tr><tr><th>Haemanthamine (18)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>52 ± 0</td><td>n.d.</td></tr><tr><th>Tetrahydromasonine (19)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>22 ± 0</td><td>n.d.</td></tr><tr><th>Hippeastrine (20)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>11 ± 2</td><td>n.d.</td></tr><tr><th>Crinine (21)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>> 500</td><td>40 ± 5</td><td>n.d.</td></tr><tr><th>Epimaritidine (22)</th><td>> 500</td><td>> 500</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Huperzine A c</th><td>0.033 ± 0.001</td><td>> 500</td><td>> 15.151</td><td>n.d.</td><td>n.d.</td><td>n.d.</td></tr><tr><th>Berberine c</th><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>142 ± 21</td><td>n.d.</td><td>n.d.</td></tr><tr><th><i>Z</i> -Pro-prolinal c</th><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>2.75 × 10−3</td><td>n.d.</td><td>n.d.</td></tr><tr><th>SB-415286 c</th><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>n.d.</td><td>70 nM</td></tr></tbody></table><p><sup>a</sup> Compound concentration required to decrease enzyme activity by 50%; the values are the mean ± SEM of three independent measurements, each performed in triplicate; <sup>b</sup> Selectivity index (SI) for <i>h</i> AChE is determined as ratio <i>h</i> BuChE IC / <i>h</i> AChE IC; <sup>c</sup> tested at 10 μM compound concentration; n.d. stands for not determined.</p>
title	Table 2 h AChE, h in Amaryllidaceae alkaloids from Narcissus pseudonarcissus L. cv. Dutch Master as potential drugs in treatment of Alzheimer's disease
topic	Biodiversity Taxonomy
url	https://doi.org/10.5281/zenodo.15158696