MotilA: A pipeline for microglial fine process motility analysis

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Autores principales: Musacchio, Fabrizio, Crux, Sophie, Nebeling, Felix, Gockel, Nala, Fuhrmann, Falko, Fuhrmann, Martin
Formato: Recurso digital
Publicado: Zenodo 2025
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author Musacchio, Fabrizio
Crux, Sophie
Nebeling, Felix
Gockel, Nala
Fuhrmann, Falko
Fuhrmann, Martin
author_facet Musacchio, Fabrizio
Crux, Sophie
Nebeling, Felix
Gockel, Nala
Fuhrmann, Falko
Fuhrmann, Martin
contents <p><a href="https://github.com/FabrizioMusacchio/MotilA" target="_blank" rel="noopener"><strong>MotilA</strong></a> is an open-source Python pipeline for the automated analysis of microglial fine process motility from 5D (t, z, channel, y, x) two-photon time-lapse imaging data. It supports both single-stack and batch analysis modes and integrates seamlessly for standardized data management.</p> <p>MotilA extracts motility metrics from segmented microglial stacks and includes preprocessing steps such as image registration and channel separation. A comprehensive example dataset is provided via <a href="https://zenodo.org/records/15061566">Zenodo</a>, along with <a href="https://github.com/FabrizioMusacchio/MotilA#example-data-set-and-tutorials" target="_blank" rel="noopener">tutorial notebooks and scripts</a> to guide new users through the analysis workflow.</p> <p><strong>Citation notice:</strong></p> <p>A peer-reviewed publication describing the MotilA pipeline in detail is currently in preparation. Until the paper is published and a DOI becomes available, please cite this Zenodo record as a reference for the software. Once the publication is available, we kindly ask users to cite the article DOI instead.</p>
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institution Zenodo
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publisher Zenodo
record_format zenodo
spellingShingle MotilA: A pipeline for microglial fine process motility analysis
Musacchio, Fabrizio
Crux, Sophie
Nebeling, Felix
Gockel, Nala
Fuhrmann, Falko
Fuhrmann, Martin
image analysis
microglia
motility
two-photon imaging
neuroscience
Python
Python pipeline
image segmentation
image processing
image registration
image analysis pipeline
<p><a href="https://github.com/FabrizioMusacchio/MotilA" target="_blank" rel="noopener"><strong>MotilA</strong></a> is an open-source Python pipeline for the automated analysis of microglial fine process motility from 5D (t, z, channel, y, x) two-photon time-lapse imaging data. It supports both single-stack and batch analysis modes and integrates seamlessly for standardized data management.</p> <p>MotilA extracts motility metrics from segmented microglial stacks and includes preprocessing steps such as image registration and channel separation. A comprehensive example dataset is provided via <a href="https://zenodo.org/records/15061566">Zenodo</a>, along with <a href="https://github.com/FabrizioMusacchio/MotilA#example-data-set-and-tutorials" target="_blank" rel="noopener">tutorial notebooks and scripts</a> to guide new users through the analysis workflow.</p> <p><strong>Citation notice:</strong></p> <p>A peer-reviewed publication describing the MotilA pipeline in detail is currently in preparation. Until the paper is published and a DOI becomes available, please cite this Zenodo record as a reference for the software. Once the publication is available, we kindly ask users to cite the article DOI instead.</p>
title MotilA: A pipeline for microglial fine process motility analysis
topic image analysis
microglia
motility
two-photon imaging
neuroscience
Python
Python pipeline
image segmentation
image processing
image registration
image analysis pipeline
url https://doi.org/10.5281/zenodo.15175054