Phytosomal Formulation of Clerodendrum Infortunatum for Anti- Tubercular Activity and Development of a Therapeutic Suspension
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2025
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| author | Archana S.*, Dr. Sapna Shrikumar |
| author_facet | Archana S.*, Dr. Sapna Shrikumar |
| contents | <p><span>Background: Tuberculosis (TB) continues to pose a significant global health challenge, necessitating the development of novel therapeutic approaches. Clerodendrum infortunatum, a medicinal plant with traditional therapeutic uses, contains bioactive constituents like flavonoids and terpenoids with known anti-tubercular properties. Phytosome technology offers a promising platform to enhance the bioavailability and efficacy of plant-derived compounds. Aim: This study aimed to formulate and evaluate phytosomes prepared from the ethanolic extract of Clerodendrum infortunatum leaves for anti-tubercular activity. Furthermore, the study focused on the development of an anti-TB suspension using the optimized phytosomal formulation. Method: The ethanolic extract of Clerodendrum infortunatum was prepared using Soxhlet extraction. The flavonoidal bio-fraction was isolated via column chromatography and used to formulate phytosomes in a 1:2 ratio (extract: soya lecithin). Phytosomes were characterized using TLC and evaluated for in vitro anti-tubercular activity using the alamarBlue assay, with isoniazid and streptomycin as standards. Anti-TB suspensions (F1, F2, F3) with varying concentrations of phytosomes were developed and assessed for sedimentation volume, viscosity, pH, and stability. Results: The phytosomes exhibited enhanced anti-TB activity compared to the crude extract, with the flavonoidal bio-fraction showing significant efficacy at 1.6 µg/mL. Among the suspensions, F2 (6.35 mg sample) demonstrated optimal parameters, including improved stability and bioactivity. Conclusion: The study highlights the potential of Clerodendrum infortunatum phytosomes as an effective anti-tubercular agent. The developed anti-TB suspension offers a promising formulation for enhanced therapeutic delivery. Further investigations are warranted to explore its clinical application.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_15183026 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | Phytosomal Formulation of Clerodendrum Infortunatum for Anti- Tubercular Activity and Development of a Therapeutic Suspension Archana S.*, Dr. Sapna Shrikumar Clerodendrum Infortunatum, Phytosomes, Tuberculosis, Anti-TB Suspension, Alamarblue Assay, Flavonoids. <p><span>Background: Tuberculosis (TB) continues to pose a significant global health challenge, necessitating the development of novel therapeutic approaches. Clerodendrum infortunatum, a medicinal plant with traditional therapeutic uses, contains bioactive constituents like flavonoids and terpenoids with known anti-tubercular properties. Phytosome technology offers a promising platform to enhance the bioavailability and efficacy of plant-derived compounds. Aim: This study aimed to formulate and evaluate phytosomes prepared from the ethanolic extract of Clerodendrum infortunatum leaves for anti-tubercular activity. Furthermore, the study focused on the development of an anti-TB suspension using the optimized phytosomal formulation. Method: The ethanolic extract of Clerodendrum infortunatum was prepared using Soxhlet extraction. The flavonoidal bio-fraction was isolated via column chromatography and used to formulate phytosomes in a 1:2 ratio (extract: soya lecithin). Phytosomes were characterized using TLC and evaluated for in vitro anti-tubercular activity using the alamarBlue assay, with isoniazid and streptomycin as standards. Anti-TB suspensions (F1, F2, F3) with varying concentrations of phytosomes were developed and assessed for sedimentation volume, viscosity, pH, and stability. Results: The phytosomes exhibited enhanced anti-TB activity compared to the crude extract, with the flavonoidal bio-fraction showing significant efficacy at 1.6 µg/mL. Among the suspensions, F2 (6.35 mg sample) demonstrated optimal parameters, including improved stability and bioactivity. Conclusion: The study highlights the potential of Clerodendrum infortunatum phytosomes as an effective anti-tubercular agent. The developed anti-TB suspension offers a promising formulation for enhanced therapeutic delivery. Further investigations are warranted to explore its clinical application.</span></p> |
| title | Phytosomal Formulation of Clerodendrum Infortunatum for Anti- Tubercular Activity and Development of a Therapeutic Suspension |
| topic | Clerodendrum Infortunatum, Phytosomes, Tuberculosis, Anti-TB Suspension, Alamarblue Assay, Flavonoids. |
| url | https://doi.org/10.5281/zenodo.15183026 |