Larvicidal and Pupicidal Activity of Green Synthesized Silver Nanoparticles Using Lawsonia Inermis against Culex Quinquefasciatus

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Main Author: Samidoss Christina Mary, Geethanandhini Selvaraj, Priyamol Varghese Sabu
Format: Recurso digital
Published: Zenodo 2025
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author Samidoss Christina Mary, Geethanandhini Selvaraj, Priyamol Varghese Sabu
author_facet Samidoss Christina Mary, Geethanandhini Selvaraj, Priyamol Varghese Sabu
contents <p>The use of chemical insecticides for mosquito control often leads to issues such as resistance<br>development, highlighting the need for alternative control methods using plant-based products. In this<br>context, the present study investigates the green synthesis of silver nanoparticles (AgNPs) using Lawsonia<br>inermis leaf extract and evaluates their larvicidal effects on the II, III, IV instars, and pupa of Culex<br>quinquefasciatus. The synthesized AgNPs were characterized using UV-Vis spectroscopy and Fourier<br>Transform Infrared Spectroscopy (FTIR). Larvae from the II, III, IV instar stages and pupa were exposed to<br>varying concentrations of silver nanoparticles (ranging from 5 ppm to 50 ppm) for 24 hours. The LC50<br>values recorded after 24 hours were 13.14 ppm for II instar, 16.52 ppm for III instar, 33.40 ppm for IV instar,<br>and 43.21 ppm for the pupa. The biosynthesis of silver nanoparticles using Lawsonia inermis leaf extract<br>offers a promising and eco-friendly approach for controlling Culex quinquefasciatus larvae.<br>Keywords — Larvicidal activity, pupicidal activity, Lawsonia inermis, Culex quinquefasciatus, green<br>synthesized silver nanoparticles</p>
format Recurso digital
id zenodo_https___doi_org_10_5281_zenodo_15213727
institution Zenodo
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publishDate 2025
publisher Zenodo
record_format zenodo
spellingShingle Larvicidal and Pupicidal Activity of Green Synthesized Silver Nanoparticles Using Lawsonia Inermis against Culex Quinquefasciatus
Samidoss Christina Mary, Geethanandhini Selvaraj, Priyamol Varghese Sabu
<p>The use of chemical insecticides for mosquito control often leads to issues such as resistance<br>development, highlighting the need for alternative control methods using plant-based products. In this<br>context, the present study investigates the green synthesis of silver nanoparticles (AgNPs) using Lawsonia<br>inermis leaf extract and evaluates their larvicidal effects on the II, III, IV instars, and pupa of Culex<br>quinquefasciatus. The synthesized AgNPs were characterized using UV-Vis spectroscopy and Fourier<br>Transform Infrared Spectroscopy (FTIR). Larvae from the II, III, IV instar stages and pupa were exposed to<br>varying concentrations of silver nanoparticles (ranging from 5 ppm to 50 ppm) for 24 hours. The LC50<br>values recorded after 24 hours were 13.14 ppm for II instar, 16.52 ppm for III instar, 33.40 ppm for IV instar,<br>and 43.21 ppm for the pupa. The biosynthesis of silver nanoparticles using Lawsonia inermis leaf extract<br>offers a promising and eco-friendly approach for controlling Culex quinquefasciatus larvae.<br>Keywords — Larvicidal activity, pupicidal activity, Lawsonia inermis, Culex quinquefasciatus, green<br>synthesized silver nanoparticles</p>
title Larvicidal and Pupicidal Activity of Green Synthesized Silver Nanoparticles Using Lawsonia Inermis against Culex Quinquefasciatus
url https://doi.org/10.5281/zenodo.15213727