Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Recurso digital |
| Language: | English |
| Published: |
Zenodo
2025
|
| Online Access: | https://doi.org/10.5281/zenodo.15370378 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- <h3><span lang="EN-US">INTRODUCTION</span></h3> <p><span lang="EN-US">This dataset provides the protocol for <em>Eragrostis nindensis</em> (<em>E. nindensis</em>) plant growth conditions, drought stress, rehydration and sampling strategies is based on previous protocols developed in Jill Farrant’s lab at the University of Cape Town</span><a title="" href="#_ftn1" name="_ftnref1"><span lang="ES"><span lang="ES">[1]</span></span></a><sup><span lang="ES">,</span></sup><sup><span lang="ES"><a title="" href="#_ftn2" name="_ftnref2"><span lang="ES">[2]</span></a></span></sup><span lang="EN-US">.</span></p> <p><span lang="EN-US">The protocol describing the preparation of nuclei followed by digestion with micrococcal nuclease I (MNAse I) and the preparation of libraries refers to the protocol originally developed for maize<a title="" href="#_ftn3" name="_ftnref3"><span lang="EN-US">[3]</span></a> and its subsequent optimization in Dr. Thomas Hartwig’s lab, Independent Research Groups, Max Planck Institute for Plant Breeding Research, Cologne, and Institute for Molecular Physiology, Cluster of Excellence on Plant Sciences, Heinrich-Heine University, Düsseldorf, Germany.</span></p> <p><span lang="FR">The figures showing nuclei digestions results are derived from the experiments, conducted by Giuseppe Trotta, University of Dusseldorf, Germany and Bojana Banovic Deri, CREA-CI, Italy, in collaboration with Sohini Mukherjee and Sergius Weize,l University of Dusseldorf, in Thomas Hartwig’s lab, </span><span lang="EN-US">Max Planck Institute for Plant Breeding Research, Cologne, Germany</span><span lang="FR">. </span></p> <p><span lang="EN-US">The bioinformatic analysis of the <em>E. nindensis</em> data has been optimized starting from the methodology initially developed for maize, as for the wet-lab protocol. The pipeline ranges from the quality control of raw reads to peak calling and differential peak calling. </span></p> <div><br> <div> <p><a title="" href="#_ftnref1" name="_ftn1"><span lang="ES"><span lang="ES">[1]</span></span></a> <span lang="ES"><a href="https://www.mdpi.com/2223-7747/14/4/531">https://www.mdpi.com/2223-7747/14/4/531</a> </span></p> </div> <div> <p><a title="" href="#_ftnref2" name="_ftn2"><span lang="ES"><span lang="ES">[2]</span></span></a><span lang="ES"><a href="https://www.researchgate.net/publication/225279941_Physiological_response_of_selected_Eragrostis_species_to_water-deficit_stress">https://www.researchgate.net/publication/225279941_Physiological_response_of_selected_Eragrostis_species_to_water-deficit_stress</a> </span></p> </div> <div> <p><a title="" href="#_ftnref3" name="_ftn3"><span lang="ES"><span lang="ES">[3]</span></span></a> <span lang="ES"><a href="https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1009689">https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1009689</a> </span><span lang="ES"> </span></p> </div> </div>