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| Autores principales: | , , , |
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| Formato: | Recurso digital |
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Zenodo
2025
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| Acceso en línea: | https://doi.org/10.5281/zenodo.15384259 |
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- <p>Translating ribosomes must wait after each elongation step for a new ternary complex EF-Tu*aa-tRNA*GTP to arrive, facilitating rapid codon recognition testing. We recently showed that this wait-time rate-limits elongation in <em>Escherichia coli</em> due to competitive combinatoric searching through crowded cytoplasm by thousands of <em>E. coli</em>'s 42 unique ternary complexes. Here, we investigate whether ribosomal L12 subunits pool translation molecules to reduce this wait time. We mimic transport and reactions underlying elongation in a physiologically accurate, physically-resolved model of crowded cytoplasm. We find that L12 pre-loading as much as doubles translation rate by reducing diffusive search time. But more L12 is not always better: faster-growing bacteria tend to have fewer L12. We resolve this apparent contradiction by demonstrating tradeoffs between binding and novel sampling as a function of copy number in <em>E. coli</em>. Variable L12 copy numbers may thus have evolved for fast or slow bacterial growth as complementary survival strategies. </p>