FIGURE 4 in HotSHOT Extraction Is a Fast, Economical, and Efficient Method for Obtaining Tardigrade gDNA
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2025
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| author | Amezcua-Martínez, Cuauhtemoc F. Jiménez-Marín, Andrea R. Dueñas-Cedillo, Alba R. Flores-Martínez, José J. Gomez-Lunar, Zulema Ruiz, Enrico A. Armendáriz-Toledano, Francisco |
| author_facet | Amezcua-Martínez, Cuauhtemoc F. Jiménez-Marín, Andrea R. Dueñas-Cedillo, Alba R. Flores-Martínez, José J. Gomez-Lunar, Zulema Ruiz, Enrico A. Armendáriz-Toledano, Francisco |
| contents | FIGURE 4: Cytochrome c oxidase I (COI) amplicons from gDNA purifed with both beads and spin columns obtained with three diferent gDNA extraction methods. R, 100-1500 bp DNA ladder; wells 1, 2, 11, and 12 correspond to Mesobiotus sp.; wells 3, 4, 13, and 14 correspond to Hypsibius cf. pallidus; wells 5, 6, 7, 15, 16, and 17 correspond to Minibiotus sidereus; wells 8, 9, 10, 18, 19, and 20 correspond to Minibiotus citlalium; "−", negative control. (a) and (b), gDNA obtained using the Qiagen kit; (c) and (d) gDNA obtained by chelating resin; (e) and (f), gDNA obtained by HotSHOT. |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_15399422 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | FIGURE 4 in HotSHOT Extraction Is a Fast, Economical, and Efficient Method for Obtaining Tardigrade gDNA Amezcua-Martínez, Cuauhtemoc F. Jiménez-Marín, Andrea R. Dueñas-Cedillo, Alba R. Flores-Martínez, José J. Gomez-Lunar, Zulema Ruiz, Enrico A. Armendáriz-Toledano, Francisco Biodiversity Taxonomy FIGURE 4: Cytochrome c oxidase I (COI) amplicons from gDNA purifed with both beads and spin columns obtained with three diferent gDNA extraction methods. R, 100-1500 bp DNA ladder; wells 1, 2, 11, and 12 correspond to Mesobiotus sp.; wells 3, 4, 13, and 14 correspond to Hypsibius cf. pallidus; wells 5, 6, 7, 15, 16, and 17 correspond to Minibiotus sidereus; wells 8, 9, 10, 18, 19, and 20 correspond to Minibiotus citlalium; "−", negative control. (a) and (b), gDNA obtained using the Qiagen kit; (c) and (d) gDNA obtained by chelating resin; (e) and (f), gDNA obtained by HotSHOT. |
| title | FIGURE 4 in HotSHOT Extraction Is a Fast, Economical, and Efficient Method for Obtaining Tardigrade gDNA |
| topic | Biodiversity Taxonomy |
| url | https://doi.org/10.5281/zenodo.15399422 |