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TABLE 2 in Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures

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Autores principales:	Bell, Karen L., Petit, Robert A., Cutler, Anya, Dobbs, Emily K., Macpherson, J. Michael, Read, Timothy D., Burgess, Kevin S., Brosi, Berry J.
Formato:	Recurso digital
Publicado:	Zenodo 2021
Materias:	Biodiversity Taxonomy
Acceso en línea:	Acceder al recurso
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author	Bell, Karen L. Petit, Robert A. Cutler, Anya Dobbs, Emily K. Macpherson, J. Michael Read, Timothy D. Burgess, Kevin S. Brosi, Berry J.
author_facet	Bell, Karen L. Petit, Robert A. Cutler, Anya Dobbs, Emily K. Macpherson, J. Michael Read, Timothy D. Burgess, Kevin S. Brosi, Berry J.
contents	<p><b>TABLE 2</b> Composition of artificial pollen mixtures used in this study</p><table><thead><tr><th><b>Mixture type</b></th><th><b>Mixture number</b></th><th><b>Species</b></th><th><b>Relative proportions in mixture (based on number of pollen grains)</b></th><th><b>Relative proportions in mixture (based on estimates of DNA quantity)</b></th><th><b>Sequencing facility and platform</b></th></tr></thead><tbody><tr><th rowspan="4">Increasing species richness (2–9)</th><td>1</td><td><i>Broussonetia papyrifera, Artemisia tridentata, Zea mays</i></td><td>0.323 5, 0.450 4, 0.226</td><td>0.084 3, 0.685 9, 0.229 8</td><td>EIGC HiSeq</td></tr><tr><td>2</td><td><i>Broussonetia papyrifera, Bassia scoparia, Artemisia tridentata, Poa pratensis, Zea mays</i></td><td>0.445 2, 0.089 7, 0.231 5, 0.173 7, 0.06</td><td>0.138 0, 0.044 5, 0.419 1, 0.326 0, 0.072 4</td><td>EIGC HiSeq</td></tr><tr><td>3</td><td><i>Populus tremuloides, Broussonetia papyrifera, Carya illinoinensis, Bassia scoparia, Artemisia tridentata, Poa pratensis, Zea mays</i></td><td>0.136 5, 0.244 8, 0.137 3, 0.071 5, 0.194 1, 0.137 5, 0.078</td><td>0.033 7, 0.084 7, 0.056 3, 0.039 6, 0.392 2, 0.288 0, 0.105 6</td><td>GGC MiSeq</td></tr><tr><td>4</td><td><i>Populus tremuloides, Populus deltoides, Broussonetia papyrifera, Carya illinoinensis, Bassia scoparia, Ambrosia artemisiifolia, Artemisia tridentata, Poa pratensis, Zea mays</i></td><td>0.178 2, 0.178 2, 0.157 1, 0.069 5, 0.028, 0.13, 0.232 7, 0.080 2, 0.046</td><td>0.045 8, 0.045 8, 0.056 5, 0.029 6, 0.016 1, 0.077 5, 0.489 2, 0.174 8, 0.064 7</td><td>GGC MiSeq</td></tr><tr><th rowspan="4">Decreasing taxonomic relatedness (congeneric-different orders)</th><td>5</td><td><i>Populus deltoides, Populus tremuloides</i></td><td>Unknown</td><td></td><td>GGC MiSeq</td></tr><tr><td>6</td><td>Poa pratensis, Zea mays</td><td>0.491 9, 0.508</td><td>0.600 6, 0.399 4</td><td>GGC MiSeq</td></tr><tr><td>7</td><td><i>Broussonetia papyrifera, Artemisia tridentata</i></td><td>0.411, 0.589</td><td>0.106 7, 0.893 3</td><td>GGC MiSeq</td></tr><tr><td>8</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.538 8, 0.461 2</td><td>0.161 7, 0.838 3</td><td>GGC MiSeq</td></tr><tr><th rowspan="4">Increasing rarity of <i>Broussonetia</i> <i>papyrifera</i> (0.5388–0.089 7 of pollen grains; 0.1617–0.016 0 of DNA in pollen grains)</th><td>8</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.538 8, 0.461 2</td><td>0.161 7, 0.838 3</td><td>GGC MiSeq</td></tr><tr><td>9</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.151 4, 0.848 6</td><td>0.028 6, 0.971 4</td><td>GGC MiSeq</td></tr><tr><td>10</td><td>Broussonetia papyrifera, Poa pratensis</td><td>0.089 7, 0.910 3</td><td>0.016 0, 0.984 0</td><td>GGC MiSeq</td></tr><tr><td>11</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.094 5, 0.905 5</td><td>0.016 9, 0.983 1</td><td>GGC MiSeq</td></tr></tbody></table>
format	Recurso digital
id	zenodo_https___doi_org_10_5281_zenodo_15856122
institution	Zenodo
language
publishDate	2021
publisher	Zenodo
record_format	zenodo
spellingShingle	TABLE 2 in Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures Bell, Karen L. Petit, Robert A. Cutler, Anya Dobbs, Emily K. Macpherson, J. Michael Read, Timothy D. Burgess, Kevin S. Brosi, Berry J. Biodiversity Taxonomy <p><b>TABLE 2</b> Composition of artificial pollen mixtures used in this study</p><table><thead><tr><th><b>Mixture type</b></th><th><b>Mixture number</b></th><th><b>Species</b></th><th><b>Relative proportions in mixture (based on number of pollen grains)</b></th><th><b>Relative proportions in mixture (based on estimates of DNA quantity)</b></th><th><b>Sequencing facility and platform</b></th></tr></thead><tbody><tr><th rowspan="4">Increasing species richness (2–9)</th><td>1</td><td><i>Broussonetia papyrifera, Artemisia tridentata, Zea mays</i></td><td>0.323 5, 0.450 4, 0.226</td><td>0.084 3, 0.685 9, 0.229 8</td><td>EIGC HiSeq</td></tr><tr><td>2</td><td><i>Broussonetia papyrifera, Bassia scoparia, Artemisia tridentata, Poa pratensis, Zea mays</i></td><td>0.445 2, 0.089 7, 0.231 5, 0.173 7, 0.06</td><td>0.138 0, 0.044 5, 0.419 1, 0.326 0, 0.072 4</td><td>EIGC HiSeq</td></tr><tr><td>3</td><td><i>Populus tremuloides, Broussonetia papyrifera, Carya illinoinensis, Bassia scoparia, Artemisia tridentata, Poa pratensis, Zea mays</i></td><td>0.136 5, 0.244 8, 0.137 3, 0.071 5, 0.194 1, 0.137 5, 0.078</td><td>0.033 7, 0.084 7, 0.056 3, 0.039 6, 0.392 2, 0.288 0, 0.105 6</td><td>GGC MiSeq</td></tr><tr><td>4</td><td><i>Populus tremuloides, Populus deltoides, Broussonetia papyrifera, Carya illinoinensis, Bassia scoparia, Ambrosia artemisiifolia, Artemisia tridentata, Poa pratensis, Zea mays</i></td><td>0.178 2, 0.178 2, 0.157 1, 0.069 5, 0.028, 0.13, 0.232 7, 0.080 2, 0.046</td><td>0.045 8, 0.045 8, 0.056 5, 0.029 6, 0.016 1, 0.077 5, 0.489 2, 0.174 8, 0.064 7</td><td>GGC MiSeq</td></tr><tr><th rowspan="4">Decreasing taxonomic relatedness (congeneric-different orders)</th><td>5</td><td><i>Populus deltoides, Populus tremuloides</i></td><td>Unknown</td><td></td><td>GGC MiSeq</td></tr><tr><td>6</td><td>Poa pratensis, Zea mays</td><td>0.491 9, 0.508</td><td>0.600 6, 0.399 4</td><td>GGC MiSeq</td></tr><tr><td>7</td><td><i>Broussonetia papyrifera, Artemisia tridentata</i></td><td>0.411, 0.589</td><td>0.106 7, 0.893 3</td><td>GGC MiSeq</td></tr><tr><td>8</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.538 8, 0.461 2</td><td>0.161 7, 0.838 3</td><td>GGC MiSeq</td></tr><tr><th rowspan="4">Increasing rarity of <i>Broussonetia</i> <i>papyrifera</i> (0.5388–0.089 7 of pollen grains; 0.1617–0.016 0 of DNA in pollen grains)</th><td>8</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.538 8, 0.461 2</td><td>0.161 7, 0.838 3</td><td>GGC MiSeq</td></tr><tr><td>9</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.151 4, 0.848 6</td><td>0.028 6, 0.971 4</td><td>GGC MiSeq</td></tr><tr><td>10</td><td>Broussonetia papyrifera, Poa pratensis</td><td>0.089 7, 0.910 3</td><td>0.016 0, 0.984 0</td><td>GGC MiSeq</td></tr><tr><td>11</td><td><i>Broussonetia papyrifera, Poa pratensis</i></td><td>0.094 5, 0.905 5</td><td>0.016 9, 0.983 1</td><td>GGC MiSeq</td></tr></tbody></table>
title	TABLE 2 in Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures
topic	Biodiversity Taxonomy
url	https://doi.org/10.5281/zenodo.15856122