5` 10x Genomics single cell RNA sequencing of CD4+ T cells from PI3K GOF and WT mice
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| Natura: | Recurso digital |
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2025
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| author | Deenick, Elissa Garvan Institute of Medical Research |
| author_facet | Deenick, Elissa Garvan Institute of Medical Research |
| contents | <p>Single cell RNA (scRNA-seq) gene expression (GEX) data for n=2 mice with PI3K GOF mutations and n=2 WT mice. scRNA-seq GEX data was generated using 5` 10x Genomics platform on flow sorted CD4+ T cells. Cells from each mouse were tagged with hashing antibodies; C301ACCCACCAGTAAGAC (WT1), C302GGTCGAGAGCATTCA (WT2), C303CTTGCCGCATGTCAT (PI3KGOF1) and C304AAGCATTCTTCACG (PI3KGOF2).</p> <p>GEX count data was generated using Cell Ranger (version 6.0.2) against mm10-3.0.0 reference. Hashtag counts were generated with CITE-seq-count (version 1.4.5) with the following parameters: --start-trim 10 --cbf 1 --cbl 16 --umif 17 --umil 28. Hashtags were demultiplexed with Seurat's HTODemux function (version 4.2.0).</p> <p> </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_15902053 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | 5` 10x Genomics single cell RNA sequencing of CD4+ T cells from PI3K GOF and WT mice Deenick, Elissa Garvan Institute of Medical Research <p>Single cell RNA (scRNA-seq) gene expression (GEX) data for n=2 mice with PI3K GOF mutations and n=2 WT mice. scRNA-seq GEX data was generated using 5` 10x Genomics platform on flow sorted CD4+ T cells. Cells from each mouse were tagged with hashing antibodies; C301ACCCACCAGTAAGAC (WT1), C302GGTCGAGAGCATTCA (WT2), C303CTTGCCGCATGTCAT (PI3KGOF1) and C304AAGCATTCTTCACG (PI3KGOF2).</p> <p>GEX count data was generated using Cell Ranger (version 6.0.2) against mm10-3.0.0 reference. Hashtag counts were generated with CITE-seq-count (version 1.4.5) with the following parameters: --start-trim 10 --cbf 1 --cbl 16 --umif 17 --umil 28. Hashtags were demultiplexed with Seurat's HTODemux function (version 4.2.0).</p> <p> </p> |
| title | 5` 10x Genomics single cell RNA sequencing of CD4+ T cells from PI3K GOF and WT mice |
| url | https://doi.org/10.5281/zenodo.15902053 |