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| Main Authors: | , |
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| Format: | Recurso digital |
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| Published: |
Zenodo
2025
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| Online Access: | https://doi.org/10.5281/zenodo.15902053 |
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Table of Contents:
- <p>Single cell RNA (scRNA-seq) gene expression (GEX) data for n=2 mice with PI3K GOF mutations and n=2 WT mice. scRNA-seq GEX data was generated using 5` 10x Genomics platform on flow sorted CD4+ T cells. Cells from each mouse were tagged with hashing antibodies; C301ACCCACCAGTAAGAC (WT1), C302GGTCGAGAGCATTCA (WT2), C303CTTGCCGCATGTCAT (PI3KGOF1) and C304AAGCATTCTTCACG (PI3KGOF2).</p> <p>GEX count data was generated using Cell Ranger (version 6.0.2) against mm10-3.0.0 reference. Hashtag counts were generated with CITE-seq-count (version 1.4.5) with the following parameters: --start-trim 10 --cbf 1 --cbl 16 --umif 17 --umil 28. Hashtags were demultiplexed with Seurat's HTODemux function (version 4.2.0).</p> <p> </p>