Comparison of dsRNA and rRNA depleted RNA methods for virome studies in complex plant communities
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| Format: | Recurso digital |
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2024
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| _version_ | 1866902282920001536 |
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| author | Niedzicka, Marta Mc Namara, Louise Byrne, Stephen |
| author_facet | Niedzicka, Marta Mc Namara, Louise Byrne, Stephen |
| contents | <p>Despite potential roles of natural plant communities as reservoirs of crop pathogens, their viromes are not well understood. The<br>choice of robust viral enrichment strategies in complex plant communities is a prerequisite if complex datasets of unknown viral<br>composition are to be understood. We use plant communities from arable field margins to compare two methods of viral nucleic<br>acid enrichment and compare three frequently used metagenomics assemblers. Both methods are well established across plant<br>virome studies; however, they have never been compared in the context of non-synthetic and complex plant communities.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_16026702 |
| institution | Zenodo |
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| publishDate | 2024 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Comparison of dsRNA and rRNA depleted RNA methods for virome studies in complex plant communities Niedzicka, Marta Mc Namara, Louise Byrne, Stephen Plant Viruses <p>Despite potential roles of natural plant communities as reservoirs of crop pathogens, their viromes are not well understood. The<br>choice of robust viral enrichment strategies in complex plant communities is a prerequisite if complex datasets of unknown viral<br>composition are to be understood. We use plant communities from arable field margins to compare two methods of viral nucleic<br>acid enrichment and compare three frequently used metagenomics assemblers. Both methods are well established across plant<br>virome studies; however, they have never been compared in the context of non-synthetic and complex plant communities.</p> |
| title | Comparison of dsRNA and rRNA depleted RNA methods for virome studies in complex plant communities |
| topic | Plant Viruses |
| url | https://doi.org/10.5281/zenodo.16026702 |