Saved in:
| Main Author: | |
|---|---|
| Format: | Recurso digital |
| Language: | |
| Published: |
Zenodo
2025
|
| Online Access: | https://doi.org/10.5281/zenodo.16312269 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- <p>Raw images (.tif), ROI files (.roi), and morphological analysis outputs (.jpg) of day 18 input organoids tested for their morphological features against reference dataset. + .csv file with the results of the morphological analysis of reference+input organoids with and without organoid size descriptors (.csv). + pca data of reference+input organoids (.xlsx)<br>Input organoids we tested on the reference pool are generated according to the following protocols: CondCO CondSWbCOEFC, CondSWbCO, CondCOnoMG, CondbCOC.</p> <p>CondCO – cerebral organoids made according to an optimized protocol with a proprietary media but lacking patterning molecules; CondCOnoMG – CO without exposure to Matrigel; CondSWbCO – guided cerebral organoid treated with SMAD WNT (TGFß and WNT) inhibitors; CondSWbCOEFC – guided cerebral organoid treated with SMAD WNT (TGFß and WNT) inhibitors plus treatment with epidermal growth factor (EGF), fibroblast growth factor (FGF), and GSK3 Inhibitor/WNT Activator (CHIR-99021) during neuroepithelial expansion; CondbCOC – unguided cerebral organoid in basal medium plus treatment with GSK3 Inhibitor/WNT Activator (CHIR-99021) during neuroepithelial expansion.<br>B=batch number</p> <p>For further details on the protocol steps refer to Chiaradia, Imaz-Rosshandler et al., 2023<sup>2</sup>.</p>