RP-HPLC Method Development and Validation for Simultaneous Estimation of Norfloxacin and Tinidazole in Combined Tablet Dosage Form
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2025
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| _version_ | 1866901740336447488 |
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| author | Mule Munjaji*, Panchbhai Vivek, Thaware Pratibha |
| author_facet | Mule Munjaji*, Panchbhai Vivek, Thaware Pratibha |
| contents | <p><span lang="EN">A simple, Accurate, precise method was developed for the simultaneous estimation of the Norfloxacin and Tinidazole in Tablet dosage form. Chromatogram was run through SunFire C18 Column3.5 µm, 4.6 mm X 150 mm. Mobile phase containing Phosphate Buffer and Acetonitrile and Methanol in the ratio of 60:30:10 was pumped through column at a flow rate of 0.8 ml/min. Temperature was maintained at 26°C. Optimized wavelength for Norfloxacin and Tinidazole was 290 nm. Retention time of Norfloxacin and Tinidazole were found to be 2.290 min and 2.848min. %RSD of the Norfloxacin and Tinidazole were and found to be 0.2 and 0.3 respectively. %Recovery was Obtained as 100.30% and 99.97% for Norfloxacin and Tinidazole. LOD, LOQ values were obtained from regression equations of Norfloxacin and Tinidazole were 0.03ppm, 0.10ppm and 0.06ppm, 0.19ppm respectively. Regression equation of Tinidazole is = 117845x + 57896, and of Norfloxacin is y = 99753x + 36144. Retention times are decreased and that run time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_16448068 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | RP-HPLC Method Development and Validation for Simultaneous Estimation of Norfloxacin and Tinidazole in Combined Tablet Dosage Form Mule Munjaji*, Panchbhai Vivek, Thaware Pratibha Norfloxacin, Tinidazole, RP-HPLC, etc <p><span lang="EN">A simple, Accurate, precise method was developed for the simultaneous estimation of the Norfloxacin and Tinidazole in Tablet dosage form. Chromatogram was run through SunFire C18 Column3.5 µm, 4.6 mm X 150 mm. Mobile phase containing Phosphate Buffer and Acetonitrile and Methanol in the ratio of 60:30:10 was pumped through column at a flow rate of 0.8 ml/min. Temperature was maintained at 26°C. Optimized wavelength for Norfloxacin and Tinidazole was 290 nm. Retention time of Norfloxacin and Tinidazole were found to be 2.290 min and 2.848min. %RSD of the Norfloxacin and Tinidazole were and found to be 0.2 and 0.3 respectively. %Recovery was Obtained as 100.30% and 99.97% for Norfloxacin and Tinidazole. LOD, LOQ values were obtained from regression equations of Norfloxacin and Tinidazole were 0.03ppm, 0.10ppm and 0.06ppm, 0.19ppm respectively. Regression equation of Tinidazole is = 117845x + 57896, and of Norfloxacin is y = 99753x + 36144. Retention times are decreased and that run time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.</span></p> |
| title | RP-HPLC Method Development and Validation for Simultaneous Estimation of Norfloxacin and Tinidazole in Combined Tablet Dosage Form |
| topic | Norfloxacin, Tinidazole, RP-HPLC, etc |
| url | https://doi.org/10.5281/zenodo.16448068 |