Sperm Motility of Cryopreserved Buffalo Semen After Thawing at Different Temperatures and Duration
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2025
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| author | Naurah Nazifah, Sri Wahjuningsih, Nurul Isnaini |
| author_facet | Naurah Nazifah, Sri Wahjuningsih, Nurul Isnaini |
| contents | <p>This study investigates the effect of different thawing temperatures and durations on the motility of<br>spermatozoa in cryopreserved buffalo semen. A total of 40 buffalo semen straws from the Lembang<br>Artificial Insemination Center (batch code AW 1205) were used. The experimental design was a<br>completely randomized design with 10 replications and 4 treatment groups: P0 (37°C for 30 seconds), P1<br>(28°C for 30 seconds), P2 (28°C for 45 seconds), and P3 (28°C for 60 seconds). Sperm motility was<br>evaluated using a microscope at 400x magnification, and motility was based on the percentage of sperm<br>moving progressively. Statistical analysis was performed using ANOVA followed by Duncan's Multiple<br>Range Test. The results showed that the highest motility (43.50%) was observed in P3 (28°C for 60<br>seconds), followed by P0 (43%), P2 (35.50%), and P1 (31.59%). These findings suggest that thawing<br>buffalo semen at 28°C for 60 seconds provides the highest sperm motility, which could improve the<br>efficiency of artificial insemination programs. </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_16788712 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Sperm Motility of Cryopreserved Buffalo Semen After Thawing at Different Temperatures and Duration Naurah Nazifah, Sri Wahjuningsih, Nurul Isnaini <p>This study investigates the effect of different thawing temperatures and durations on the motility of<br>spermatozoa in cryopreserved buffalo semen. A total of 40 buffalo semen straws from the Lembang<br>Artificial Insemination Center (batch code AW 1205) were used. The experimental design was a<br>completely randomized design with 10 replications and 4 treatment groups: P0 (37°C for 30 seconds), P1<br>(28°C for 30 seconds), P2 (28°C for 45 seconds), and P3 (28°C for 60 seconds). Sperm motility was<br>evaluated using a microscope at 400x magnification, and motility was based on the percentage of sperm<br>moving progressively. Statistical analysis was performed using ANOVA followed by Duncan's Multiple<br>Range Test. The results showed that the highest motility (43.50%) was observed in P3 (28°C for 60<br>seconds), followed by P0 (43%), P2 (35.50%), and P1 (31.59%). These findings suggest that thawing<br>buffalo semen at 28°C for 60 seconds provides the highest sperm motility, which could improve the<br>efficiency of artificial insemination programs. </p> |
| title | Sperm Motility of Cryopreserved Buffalo Semen After Thawing at Different Temperatures and Duration |
| url | https://doi.org/10.5281/zenodo.16788712 |