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| Main Authors: | , , , , , , , , |
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| Format: | Recurso digital |
| Language: | |
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Zenodo
2025
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| Online Access: | https://doi.org/10.5281/zenodo.16948753 |
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Table of Contents:
- <p>Background Micro- and nano-plastic particles (MNPs) are found in water, soil, air, food, and also in humans. There is an urgent need to understand the risks of MNPs in allergic diseases, since they represent a perfect carrier for contaminants and allergens. Here we investigated how different food allergens bind to pristine polyethylene terephthalate nanoplastic particles (nPET), their fate through the first responders, epithelial barrier and antigen-presenting cells, and whether they can augment allergic responses Method Shrimp tropomyosin (TMP), and bovine lactoferrin (LF), unlabeled or with AF647 dye and pristine nPET, produced by precipitation method, were used for the formation of nPET-allergen hard and soft corona. The protein amount bonded to nPET was determined by BCA assay. The effect of nPET on the transport of AF647 TPM and AF647 LF through the epithelial barrier was investigated by Caco-2 monolayer formation in the presence of NPs. The effect on uptake of nPET-<span>allergen corona by monocyte-derived dendritic cells (mdDCs) from healthy donors was followed by flow cytometry and SDS-PAGE. In addition, allergenicity assessment of nPET-LF hard and soft corona was performed in a basophil activation test (BAT) with three mammalian meat allergic patients and one healthy control. Results The amount of allergens that bind to nPET in the hard corona was determined to be 0.08 μg TPM/ μg nPET, and 1 μg LF /μg nPET. Furthermore, the transport of TPM and LF through the Caco-2 monolayer was slightly affected by nPET, where Caco-2 differentiated with nPET transported 1.25 times more TPM and LF than in the control Caco-2 cells. In addition, we found that nPET with and without allergen hard corona were taken up by mdDCs, and that uptake of allergen in the corona was slightly increased in comparison to allergen only, as determined by fluorescence detection in flow cytometry and SDS-PAGE from lysed cells. Importantly, in BAT nPET-LF showed higher allergenic activity than LF itself in the allergic patients, while no basophil activation was observed with nPET alone or in the healthy control. Conclusion Here we show that nPET nanoplastic can bind food allergens and augment the allergic response by affecting the transport of allergen through the epithelial barrier, the uptake and allergen presentation by mdDCs, and increasing the allergenic activity of effector cells.</span></p>